TY - JOUR T1 - Essential functions of Runx/Cbfβ in gut conventional dendritic cells for priming Rorγt<sup>+</sup> T cells JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201900441 VL - 3 IS - 1 SP - e201900441 AU - Mari Tenno AU - Alicia Yoke Wei Wong AU - Mika Ikegaya AU - Eiji Miyauchi AU - Wooseok Seo AU - Peter See AU - Tamotsu Kato AU - Takashi Taida AU - Michiko Ohno-Oishi AU - Hiroshi Ohno AU - Hideyuki Yoshida AU - Florent Ginhoux AU - Ichiro Taniuchi Y1 - 2020/01/01 UR - https://www.life-science-alliance.org/content/3/1/e201900441.abstract N2 - Acquired immune responses are initiated by activation of CD4+ helper T (Th) cells via recognition of antigens presented by conventional dendritic cells (cDCs). DCs instruct Th-cell polarization program into specific effector Th subset, which will dictate the type of immune responses. Hence, it is important to unravel how differentiation and/or activation of DC are linked with Th-cell–intrinsic mechanism that directs differentiation toward a specific effector Th subset. Here, we show that loss of Runx/Cbfβ transcription factors complexes during DC development leads to loss of CD103+CD11b+ cDC2s and alters characteristics of CD103−CD11b+ cDCs in the intestine, which was accompanied with impaired differentiation of Rorγt+ Th17 cells and type 3 Rorγt+ regulatory T cells. We also show that a Runx-binding enhancer in the Rorc gene is essential for T cells to integrate cDC-derived signals to induce Rorγt expression. These findings reveal that Runx/Cbfβ complexes play crucial and complementary roles in cDCs and Th cells to shape converging type 3 immune responses. ER -