RT Journal Article SR Electronic T1 Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signalling JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e201900422 DO 10.26508/lsa.201900422 VO 2 IS 6 A1 Alessia Landi A1 Muriel Mari A1 Svenja Kleiser A1 Tobias Wolf A1 Christine Gretzmeier A1 Isabel Wilhelm A1 Dimitra Kiritsi A1 Roland Thünauer A1 Roger Geiger A1 Alexander Nyström A1 Fulvio Reggiori A1 Julie Claudinon A1 Winfried Römer YR 2019 UL https://www.life-science-alliance.org/content/2/6/e201900422.abstract AB Lectins are glycan-binding proteins with no catalytic activity and ubiquitously expressed in nature. Numerous bacteria use lectins to efficiently bind to epithelia, thus facilitating tissue colonisation. Wounded skin is one of the preferred niches for Pseudomonas aeruginosa, which has developed diverse strategies to impair tissue repair processes and promote infection. Here, we analyse the effect of the P. aeruginosa fucose-binding lectin LecB on human keratinocytes and demonstrate that it triggers events in the host, upon binding to fucosylated residues on cell membrane receptors, which extend beyond its role as an adhesion molecule. We found that LecB associates with insulin-like growth factor-1 receptor and dampens its signalling, leading to the arrest of cell cycle. In addition, we describe a novel LecB-triggered mechanism to down-regulate host cell receptors by showing that LecB leads to insulin-like growth factor-1 receptor internalisation and subsequent missorting towards intracellular endosomal compartments, without receptor activation. Overall, these data highlight that LecB is a multitask virulence factor that, through subversion of several host pathways, has a profound impact on keratinocyte proliferation and survival.