RT Journal Article
SR Electronic
T1 Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900433
DO 10.26508/lsa.201900433
VO 2
IS 5
A1 Ait-Saada, Anissia
A1 Khorosjutina, Olga
A1 Chen, Jiang
A1 Kramarz, Karol
A1 Maksimov, Vladimir
A1 Svensson, J Peter
A1 Lambert, Sarah
A1 Ekwall, Karl
YR 2019
UL https://www.life-science-alliance.org/content/2/5/e201900433.abstract
AB Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for single-strand annealing of an induced double-strand break. Furthermore, we use an inducible replication fork barrier system to show that Fft3 has two distinct roles at blocked DNA replication forks. First, Fft3 is needed for the resection of nascent strands, and second, it is required to restart the blocked forks. The latter function is independent of its ATPase activity.