TY - JOUR T1 - Reconstructing B-cell receptor sequences from short-read single-cell RNA sequencing with BRAPeS JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201900371 VL - 2 IS - 4 SP - e201900371 AU - Shaked Afik AU - Gabriel Raulet AU - Nir Yosef Y1 - 2019/08/01 UR - https://www.life-science-alliance.org/content/2/4/e201900371.abstract N2 - RNA sequencing of single B cells provides simultaneous measurements of the cell state and its antigen specificity as determined by the B-cell receptor (BCR). However, to uncover the latter, further reconstruction of the BCR sequence is needed. We present BRAPeS (“BCR Reconstruction Algorithm for Paired-end Single cells” ), an algorithm for reconstructing BCRs from short-read paired-end single-cell RNA sequencing. BRAPeS is accurate and achieves a high success rate even at very short (25 bp) read length, which can decrease the cost and increase the number of cells that can be analyzed compared with long reads. BRAPeS is publicly available at the following link: https://github.com/YosefLab/BRAPeS. ER -