RT Journal Article SR Electronic T1 Reconstructing B-cell receptor sequences from short-read single-cell RNA sequencing with BRAPeS JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e201900371 DO 10.26508/lsa.201900371 VO 2 IS 4 A1 Afik, Shaked A1 Raulet, Gabriel A1 Yosef, Nir YR 2019 UL https://www.life-science-alliance.org/content/2/4/e201900371.abstract AB RNA sequencing of single B cells provides simultaneous measurements of the cell state and its antigen specificity as determined by the B-cell receptor (BCR). However, to uncover the latter, further reconstruction of the BCR sequence is needed. We present BRAPeS (“BCR Reconstruction Algorithm for Paired-end Single cells” ), an algorithm for reconstructing BCRs from short-read paired-end single-cell RNA sequencing. BRAPeS is accurate and achieves a high success rate even at very short (25 bp) read length, which can decrease the cost and increase the number of cells that can be analyzed compared with long reads. BRAPeS is publicly available at the following link: https://github.com/YosefLab/BRAPeS.