PT  - JOURNAL ARTICLE
AU  - Shaked Afik
AU  - Gabriel Raulet
AU  - Nir Yosef
TI  - Reconstructing B-cell receptor sequences from short-read single-cell RNA sequencing with BRAPeS
AID  - 10.26508/lsa.201900371
DP  - 2019 Aug 01
TA  - Life Science Alliance
PG  - e201900371
VI  - 2
IP  - 4
4099  - https://www.life-science-alliance.org/content/2/4/e201900371.short
4100  - https://www.life-science-alliance.org/content/2/4/e201900371.full
SO  - Life Sci. Alliance2019 Aug 01; 2
AB  - RNA sequencing of single B cells provides simultaneous measurements of the cell state and its antigen specificity as determined by the B-cell receptor (BCR). However, to uncover the latter, further reconstruction of the BCR sequence is needed. We present BRAPeS (“BCR Reconstruction Algorithm for Paired-end Single cells” ), an algorithm for reconstructing BCRs from short-read paired-end single-cell RNA sequencing. BRAPeS is accurate and achieves a high success rate even at very short (25 bp) read length, which can decrease the cost and increase the number of cells that can be analyzed compared with long reads. BRAPeS is publicly available at the following link: https://github.com/YosefLab/BRAPeS.