RT Journal Article
SR Electronic
T1 Subunit interactions and arrangements in the fission yeast Mis16–Mis18–Mis19 complex
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201900408
DO 10.26508/lsa.201900408
VO 2
IS 4
A1 Korntner-Vetter, Melanie
A1 Lefèvre, Stéphane
A1 Hu, Xiao-Wen
A1 George, Roger
A1 Singleton, Martin R
YR 2019
UL https://www.life-science-alliance.org/content/2/4/e201900408.abstract
AB Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1CENP-A. Cell cycle–specific deposition of Cnp1 requires the Mis16–Mis18–Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18–Mis19 interface.