PT  - JOURNAL ARTICLE
AU  - Korntner-Vetter, Melanie
AU  - Lefèvre, Stéphane
AU  - Hu, Xiao-Wen
AU  - George, Roger
AU  - Singleton, Martin R
TI  - Subunit interactions and arrangements in the fission yeast Mis16–Mis18–Mis19 complex
AID  - 10.26508/lsa.201900408
DP  - 2019 Aug 01
TA  - Life Science Alliance
PG  - e201900408
VI  - 2
IP  - 4
4099  - https://www.life-science-alliance.org/content/2/4/e201900408.short
4100  - https://www.life-science-alliance.org/content/2/4/e201900408.full
SO  - Life Sci. Alliance2019 Aug 01; 2
AB  - Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1CENP-A. Cell cycle–specific deposition of Cnp1 requires the Mis16–Mis18–Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18–Mis19 interface.