RT Journal Article SR Electronic T1 TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay JF Life Science Alliance JO Life Sci. Alliance FD Life Science Alliance LLC SP e201900318 DO 10.26508/lsa.201900318 VO 2 IS 4 A1 Junaid Akhtar A1 Piyush More A1 Steffen Albrecht A1 Federico Marini A1 Waldemar Kaiser A1 Apurva Kulkarni A1 Leszek Wojnowski A1 Jean-Fred Fontaine A1 Miguel A Andrade-Navarro A1 Marion Silies A1 Christian Berger YR 2019 UL https://www.life-science-alliance.org/content/2/4/e201900318.abstract AB Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.