%0 Journal Article %A Junaid Akhtar %A Piyush More %A Steffen Albrecht %A Federico Marini %A Waldemar Kaiser %A Apurva Kulkarni %A Leszek Wojnowski %A Jean-Fred Fontaine %A Miguel A Andrade-Navarro %A Marion Silies %A Christian Berger %T TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay %D 2019 %R 10.26508/lsa.201900318 %J Life Science Alliance %P e201900318 %V 2 %N 4 %X Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach. %U https://www.life-science-alliance.org/content/lsa/2/4/e201900318.full.pdf