TY - JOUR T1 - Dietary restriction induces posttranscriptional regulation of longevity genes JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201800281 VL - 2 IS - 4 SP - e201800281 AU - Jarod A Rollins AU - Dan Shaffer AU - Santina S Snow AU - Pankaj Kapahi AU - Aric N Rogers Y1 - 2019/08/01 UR - https://www.life-science-alliance.org/content/2/4/e201800281.abstract N2 - Dietary restriction (DR) increases life span through adaptive changes in gene expression. To understand more about these changes, we analyzed the transcriptome and translatome of Caenorhabditis elegans subjected to DR. Transcription of muscle regulatory and structural genes increased, whereas increased expression of amino acid metabolism and neuropeptide signaling genes was controlled at the level of translation. Evaluation of posttranscriptional regulation identified putative roles for RNA-binding proteins, RNA editing, miRNA, alternative splicing, and nonsense-mediated decay in response to nutrient limitation. Using RNA interference, we discovered several differentially expressed genes that regulate life span. We also found a compensatory role for translational regulation, which offsets dampened expression of a large subset of transcriptionally down-regulated genes. Furthermore, 3′ UTR editing and intron retention increase under DR and correlate with diminished translation, whereas trans-spliced genes are refractory to reduced translation efficiency compared with messages with the native 5′ UTR. Finally, we find that smg-6 and smg-7, which are genes governing selection and turnover of nonsense-mediated decay targets, are required for increased life span under DR. ER -