RT Journal Article
SR Electronic
T1 Robust repression of tRNA gene transcription during stress requires protein arginine methylation
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800261
DO 10.26508/lsa.201800261
VO 2
IS 3
A1 Richoo B Davis
A1 Neah Likhite
A1 Christopher A Jackson
A1 Tao Liu
A1 Michael C Yu
YR 2019
UL https://www.life-science-alliance.org/content/2/3/e201800261.abstract
AB Protein arginine methylation is an important means by which protein function can be regulated. In the budding yeast, this modification is catalyzed by the major protein arginine methyltransferase Hmt1. Here, we provide evidence that the Hmt1-mediated methylation of Rpc31, a subunit of RNA polymerase III, plays context-dependent roles in tRNA gene transcription: under conditions optimal for growth, it positively regulates tRNA gene transcription, and in the setting of stress, it promotes robust transcriptional repression. In the context of stress, methylation of Rpc31 allows for its optimal interaction with RNA polymerase III global repressor Maf1. Interestingly, mammalian Hmt1 homologue is able to methylate one of Rpc31’s human homologue, RPC32β, but not its paralogue, RPC32α. Our data led us to propose an efficient model whereby protein arginine methylation facilitates metabolic economy and coordinates protein-synthetic capacity.