TY - JOUR T1 - Development of a new monoclonal antibody specific to mouse Vγ6 chain JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201900363 VL - 2 IS - 3 SP - e201900363 AU - Shinya Hatano AU - Xin Tun AU - Naoto Noguchi AU - Dan Yue AU - Hisakata Yamada AU - Xun Sun AU - Mitsuru Matsumoto AU - Yasunobu Yoshikai Y1 - 2019/06/01 UR - https://www.life-science-alliance.org/content/2/3/e201900363.abstract N2 - There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene–transfected T-cell line lacking TCR as well as of Vγ1− Vγ4− Vγ5− Vγ7− γδ T cells and the CD3high TCRδint γδ T cells in various organs. The location of Vγ6+ γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice. ER -