TY - JOUR T1 - Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201800195 VL - 2 IS - 2 SP - e201800195 AU - Masashi Matsuda AU - Rintaro Ono AU - Tomonori Iyoda AU - Takaho Endo AU - Makoto Iwasaki AU - Mariko Tomizawa-Murasawa AU - Yoriko Saito AU - Akiko Kaneko AU - Kanako Shimizu AU - Daisuke Yamada AU - Narumi Ogonuki AU - Takashi Watanabe AU - Manabu Nakayama AU - Yoko Koseki AU - Fuyuko Kezuka-Shiotani AU - Takanori Hasegawa AU - Hiromasa Yabe AU - Shunichi Kato AU - Atsuo Ogura AU - Leonard D Shultz AU - Osamu Ohara AU - Masaru Taniguchi AU - Haruhiko Koseki AU - Shin-ichiro Fujii AU - Fumihiko Ishikawa Y1 - 2019/04/01 UR - https://www.life-science-alliance.org/content/2/2/e201800195.abstract N2 - The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells. ER -