TY - JOUR T1 - The small and large ribosomal subunits depend on each other for stability and accumulation JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201800150 VL - 2 IS - 2 SP - e201800150 AU - Brian Gregory AU - Nusrat Rahman AU - Ananth Bommakanti AU - Md Shamsuzzaman AU - Mamata Thapa AU - Alana Lescure AU - Janice M Zengel AU - Lasse Lindahl Y1 - 2019/04/01 UR - https://www.life-science-alliance.org/content/2/2/e201800150.abstract N2 - The 1:1 balance between the numbers of large and small ribosomal subunits can be disturbed by mutations that inhibit the assembly of only one of the subunits. Here, we have investigated if the cell can counteract an imbalance of the number of the two subunits. We show that abrogating 60S assembly blocks 40S subunit accumulation. In contrast, cessation of the 40S pathways does not prevent 60S accumulation, but does, however, lead to fragmentation of the 25S rRNA in 60S subunits and formation of a 55S ribosomal particle derived from the 60S. We also present evidence suggesting that these events occur post assembly and discuss the possibility that the turnover of subunits is due to vulnerability of free subunits not paired with the other subunit to form 80S ribosomes. ER -