RT Journal Article
SR Electronic
T1 Reciprocal regulation of STING and TCR signaling by mTORC1 for T-cell activation and function
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800282
DO 10.26508/lsa.201800282
VO 2
IS 1
A1 Imanishi, Takayuki
A1 Unno, Midori
A1 Kobayashi, Wakana
A1 Yoneda, Natsumi
A1 Matsuda, Satoshi
A1 Ikeda, Kazutaka
A1 Hoshii, Takayuki
A1 Hirao, Atsushi
A1 Miyake, Kensuke
A1 Barber, Glen N
A1 Arita, Makoto
A1 Ishii, Ken J
A1 Akira, Shizuo
A1 Saito, Takashi
YR 2019
UL http://www.life-science-alliance.org/content/2/1/e201800282.abstract
AB Stimulator of interferon genes (STING) plays a key role in detecting cytosolic DNA and induces type I interferon (IFN-I) responses for host defense against pathogens. Although T cells highly express STING, its physiological role remains unknown. Here, we show that costimulation of T cells with the STING ligand cGAMP and TCR leads to IFN-I production and strongly inhibits T-cell growth. TCR-mediated mTORC1 activation and sustained activation of IRF3 are required for cGAMP-induced IFN-I production, and the mTORC1 activity is partially counteracted by cGAMP, thereby blocking proliferation. This mTORC1 inhibition in response to costimulation depends on IRF3 and IRF7. Effector T cells produce much higher IFN-I levels than innate cells in response to cGAMP. Finally, we demonstrated that STING stimulation in T cells is effective in inducing antitumor responses in vivo. Our studies demonstrate that the outputs of STING and TCR signaling pathways are mutually regulated through mTORC1 to modulate T-cell functions.