RT Journal Article
SR Electronic
T1 Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DC
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800093
DO 10.26508/lsa.201800093
VO 1
IS 6
A1 Schierer, Stefan
A1 Ostalecki, Christian
A1 Zinser, Elisabeth
A1 Lamprecht, Ricarda
A1 Plosnita, Bianca
A1 Stich, Lena
A1 Dörrie, Jan
A1 Lutz, Manfred B
A1 Schuler, Gerold
A1 Baur, Andreas S
YR 2018
UL http://www.life-science-alliance.org/content/1/6/e201800093.abstract
AB During inflammation, murine and human monocytes can develop into dendritic cells (DC), but this process is not entirely understood. Here, we demonstrate that extracellular vesicles (EV) secreted by mature human DC (maDC) differentiate peripheral monocytes into immature DC, expressing a unique marker pattern, including 6-sulfo LacNAc (slan), Zbtb46, CD64, and CD14. While EV from both maDC and immature DC differentiated monocytes similar to GM-CSF/IL-4 stimulation, only maDC-EV produced precursors, which upon maturation stimulus developed into T-cell–activating and IL-12p70–secreting maDC. Mechanistically, maDC-EV induced cell signaling through GM-CSF, which was abundant in EV as were IL-4 and other cytokines and chemokines. When injected into the mouse skin, murine maDC-EV attracted immune cells including monocytes that developed activation markers typical for inflammatory cells. Skin-injected EV also reached lymph nodes, causing a similar immune cell infiltration. We conclude that DC-derived EV likely serve to perpetuate an immune reaction and may contribute to chronic inflammation.