PT  - JOURNAL ARTICLE
AU  - Soula, Anaïs
AU  - Valere, Mélissa
AU  - López-González, María-José
AU  - Ury-Thiery, Vicky
AU  - Groppi, Alexis
AU  - Landry, Marc
AU  - Nikolski, Macha
AU  - Favereaux, Alexandre
TI  - Small RNA-Seq reveals novel miRNAs shaping the transcriptomic identity of rat brain structures
AID  - 10.26508/lsa.201800018
DP  - 2018 Oct 01
TA  - Life Science Alliance
PG  - e201800018
VI  - 1
IP  - 5
4099  - http://www.life-science-alliance.org/content/1/5/e201800018.short
4100  - http://www.life-science-alliance.org/content/1/5/e201800018.full
SO  - Life Sci. Alliance2018 Oct 01; 1
AB  - In the central nervous system (CNS), miRNAs are involved in key functions, such as neurogenesis and synaptic plasticity. Moreover, they are essential to define specific transcriptomes in tissues and cells. However, few studies were performed to determine the miRNome of the different structures of the rat CNS, although a major model in neuroscience. Here, we determined by small RNA-Seq, the miRNome of the olfactory bulb, the hippocampus, the cortex, the striatum, and the spinal cord and showed the expression of 365 known miRNAs and 90 novel miRNAs. Differential expression analysis showed that several miRNAs were specifically enriched/depleted in these CNS structures. Transcriptome analysis by mRNA-Seq and correlation based on miRNA target predictions suggest that the specifically enriched/depleted miRNAs have a strong impact on the transcriptomic identity of the CNS structures. Altogether, these results suggest the critical role played by these enriched/depleted miRNAs, in particular the novel miRNAs, in the functional identities of CNS structures.