RT Journal Article
SR Electronic
T1 Mitochondrial ubiquinone–mediated longevity is marked by reduced cytoplasmic mRNA translation
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e201800082
DO 10.26508/lsa.201800082
VO 1
IS 5
A1 Marte Molenaars
A1 Georges E Janssens
A1 Toon Santermans
A1 Marco Lezzerini
A1 Rob Jelier
A1 Alyson W MacInnes
A1 Riekelt H Houtkooper
YR 2018
UL https://www.life-science-alliance.org/content/1/5/e201800082.abstract
AB Mutations in the clk-1 gene impair mitochondrial ubiquinone biosynthesis and extend the lifespan in Caenorhabditis elegans. We demonstrate here that this life extension is linked to the repression of cytoplasmic mRNA translation, independent of the alleged nuclear form of CLK-1. Clk-1 mutations inhibit polyribosome formation similarly to daf-2 mutations that dampen insulin signaling. Comparisons of total versus polysomal RNAs in clk-1(qm30) mutants reveal a reduction in the translational efficiencies of mRNAs coding for elements of the translation machinery and an increase in those coding for the oxidative phosphorylation and autophagy pathways. Knocking down the transcription initiation factor TATA-binding protein-associated factor 4, a protein that becomes sequestered in the cytoplasm during early embryogenesis to induce transcriptional silencing, ameliorates the clk-1 inhibition of polyribosome formation. These results underscore a prominent role for the repression of cytoplasmic protein synthesis in eukaryotic lifespan extension and suggest that mutations impairing mitochondrial function are able to exploit this repression similarly to reductions of insulin signaling. Moreover, this report reveals an unexpected role for TATA-binding protein-associated factor 4 as a repressor of polyribosome formation when ubiquinone biosynthesis is compromised.