TY - JOUR T1 - Neuron-specific inactivation of <em>Wt1</em> alters locomotion in mice and changes interneuron composition in the spinal cord JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.201800106 VL - 1 IS - 4 SP - e201800106 AU - Danny Schnerwitzki AU - Sharn Perry AU - Anna Ivanova AU - Fabio V Caixeta AU - Paul Cramer AU - Sven Günther AU - Kathrin Weber AU - Atieh Tafreshiha AU - Lore Becker AU - Ingrid L Vargas Panesso AU - Thomas Klopstock AU - Martin Hrabe de Angelis AU - Manuela Schmidt AU - Klas Kullander AU - Christoph Englert Y1 - 2018/08/01 UR - https://www.life-science-alliance.org/content/1/4/e201800106.abstract N2 - Locomotion is coordinated by neuronal circuits of the spinal cord. Recently, dI6 neurons were shown to participate in the control of locomotion. A subpopulation of dI6 neurons expresses the Wilms tumor suppressor gene Wt1. However, the function of Wt1 in these cells is not understood. Here, we aimed to identify behavioral changes and cellular alterations in the spinal cord associated with Wt1 deletion. Locomotion analyses of mice with neuron-specific Wt1 deletion revealed a slower walk with a decreased stride frequency and an increased stride length. These mice showed changes in their fore-/hindlimb coordination, which were accompanied by a loss of contralateral projections in the spinal cord. Neonates with Wt1 deletion displayed an increase in uncoordinated hindlimb movements and their motor neuron output was arrhythmic with a decreased frequency. The population size of dI6, V0, and V2a neurons in the developing spinal cord of conditional Wt1 mutants was significantly altered. These results show that the development of particular dI6 neurons depends on Wt1 expression and that loss of Wt1 is associated with alterations in locomotion. ER -