Genomics & Functional Genomics
A genome-wide CRISPR functional survey of the human phagocytosis molecular machineryThe validation and interpretation of a genome-wide CRISRPko FACS screen via protein–protein interaction data yield a broad view of the molecular network-regulating phagocytosis in human cells.
Identifying novel regulators of placental development using time-series transcriptome dataNetwork analysis of RNA-seq data generated from mouse placenta at embryonic days 7.5, 8.5, and 9.5 identifies novel regulators of placental development.
Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identityLeptospira strains show very large antigenic variation (serovars). Here, the rfb gene cluster is shown to be transferred horizontally within/among Leptospira species, discriminating serovar identity.
Pandemic Vibrio cholerae acquired competitive traits from an environmental Vibrio speciesPandemic-associated Vibrio cholerae interbacterial defense genes were likely acquired through competition with and subsequent gene transfer from the fish pathogen Vibrio anguillarum.
Synergistic activation of RARβ and RARγ nuclear receptors restores cell specialization during stem cell differentiation by hijacking RARα-controlled programsSynergistic activation of RARβ and RARγ nuclear receptors in mouse stem cells restores cell specialization during neuronal differentiation by hijacking RARα-controlled programs.
Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUNWe present a detailed step-wise protocol for reliable genome-wide profiling of histone modifications using CUT&RUN that requires only low numbers of Plasmodium falciparum blood stages.
Loss of autism-candidate CHD8 perturbs neural crest development and intestinal homeostatic balanceA combination of zebrafish and transcriptomic analyses revealed the role of the autism-candidate chd8 in the development of the enteric nervous system, serotonin metabolism, and intestinal integrity.
C-to-U RNA deamination is the driving force accelerating SARS-CoV-2 evolutionThe authors propose that the host-mediated C-to-U RNA editing event, which is also affected by the RNA structure, is the driving force that accelerates SARS-CoV-2 mutation and evolution.
Myeloma immunoglobulin rearrangement and translocation detection through targeted capture sequencingTargeted capture sequencing (CapIG-seq) is a novel, efficient, and effective approach to examine multiple genomic alterations in ctDNA and bone marrow aspirates of patients with multiple myeloma.
CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tauUsing a genome-wide CRISPRi screen, we identified ANKLE2, BANF1, NUSAP1, EIF1AD, and VPS18 as novel regulators of tau pathology induced by both exosome-like extracellular vesicles and free tau seeds.