Genomics & Functional Genomics
CRISPR-induced double-strand breaks trigger recombination between homologous chromosome armsCRISPR–Cas9 enables recombination between homologous chromosome arms at predefined sites and also underscores the need for caution when applying CRISPR technologies in translational medicine.
Rate of brain aging and APOE ε4 are synergistic risk factors for Alzheimer’s diseaseThis study describes a gauge for brain aging predictive of cognitive decline and Alzheimer's disease synergistic with the APOE ε4 allele.
A network of human functional gene interactions from knockout fitness screens in cancer cellsThe function of human genes can be strongly inferred from their knockout fitness profiles across hundreds of CRISPR screens, illuminating the modular organization of the cell.
Intra-individual methylomics detects the impact of early-life adversityThis study shows that methylation profile changes across time in the same individual distinguish a stressful experience from typical infancy, providing a potential predictive marker of vulnerability to disease.
Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elementsThis study shows that addition of a single transcription factor binding site can be sufficient to convert a gene into a glucocorticoid receptor target.
A junction coverage compatibility score to quantify the reliability of transcript abundance estimates and annotation catalogsComparison of observed exon–exon junction counts to those predicted from estimated transcript abundances can identify genes with misannotated or misquantified transcripts.
Evolution of gene expression levels in the male reproductive organs of Anopheles mosquitoesExpression patterns of transcripts in reproductive tissues reveal the phylogeny of the An. gambiae complex and are indicative of molecular function, transcription regulatory networks, and protein–protein interaction networks.
Optimized ChIP-seq method facilitates transcription factor profiling in human tumorsThis study presents an optimized ChIP-seq protocol to enhance transcription factor profiling in human tumours, enabling the analysis of highly challenging samples, including core needle biopsies.
Re-annotation of eight Drosophila genomesUsing a well-annotated genome to inform the annotation of genomes in a phylogeny.
A carnivorous plant genetic map: pitcher/insect-capture QTL on a genetic linkage map of SarraceniaThis study presents the first genetic map for a carnivorous plant, mapping 64 QTLs in Sarracenia to provide the genetic basis for differences between the pitfall and lobster-trap insect-capture strategies.