Development
PARP-1 is a transcriptional rheostat of metabolic and bivalent genes during developmentPARP-1 fine-tunes gene expression to control energy balance and development by selectively repressing and activating critical genes for developmental progression.
Transgenerational epigenetic effects imposed by neonicotinoid thiacloprid exposureWe exposed pregnant female mice to neonicotinoid thiacloprid. We observed reproductive defects together with DNA methylation and histone H3K9me3 changes in the third-generation males.
Spt5 interacts genetically with Myc and is limiting for brain tumor growth in DrosophilaKnockdown of Spt5 in Drosophila affects various Myc-dependent phenotypes and delays the growth of a Myc-dependent brain tumor, thereby significantly extending the longevity of tumorous animals.
Characterization of Na+ currents regulating intrinsic excitability of optic tectal neuronsThis study examines how developing optic tectal neurons adapt their intrinsic excitability. Changes in Nav 1.1 and 1.6 expressions correlate with changes in intrinsic excitability, whereas dynamic regulation of Nav1.6 is central for intrinsic plasticity and normal sensory circuit development.
Automated staging of zebrafish embryos with deep learningIntroducing KimmelNet: A deep learning model that accurately stages populations of zebrafish embryos from 2D brightfield images, offering a rapid and objective alternative to manual observations.
MICA: a multi-omics method to predict gene regulatory networks in early human embryosOur comparative analysis of gene regulatory network predictions defines a pipeline to infer transcription factor–target gene regulatory interactions in early human embryos. Mutual information refined by chromatin accessibility allowed us to construct the first network during early human development.
Role of ciliopathy protein TMEM107 in eye development: insights from a mouse model and retinal organoidTMEM107 plays a crucial role in early vertebrate eye development and ciliogenesis in the differentiating retina, and its absence leads to eye defects and impaired retinal organoid formation.
Assessing MAPPs assay as a tool to predict the immunogenicity potential of protein therapeuticsThis work highlights the use of streptavidin magnetic beads as a valuable approach for the immunoaffinity purification of mass spectrometry MHCII-eluted ligands (MAPPs) and their practical application in assessing the drug-induced immunogenicity risk of novel biotherapeutics.
SBDSR126T rescues survival of sbds−/− zebrafish in a dose-dependent manner independently of Tp53We created a transgenic line expressing the disease-associated mutation SBDSR126T in the sbds KO zebrafish background. We demonstrated that amount of SBDSR126T is important for development and tp53 pathway activation. Tp53M214K did not rescue neutropenia or survival in the sbds-null zebrafish.
Fn14 promotes myoblast fusion during regenerative myogenesisGenetic and molecular approaches demonstrate that Fn14 receptor–mediated signaling improves myoblast fusion during skeletal muscle regeneration in a cell-autonomous manner.