The different activities of RNA G-quadruplex structures are controlled by flanking sequences

This study demonstrates the dynamic and multifunctional aspects of RNA G4 structures.


Introduction
RNA-protein interactions are key regulators of the selective processing of RNAs which includes RNA splicing, localisation, translation, and stability, and are therefore involved in various cellular processes (Lewis et al, 2017;Duss et al, 2019;Rodgers & Woodson, 2019) including embryonic development (Beaudoin et al, 2018), neuronal activity (Lin et al, 2020), and oncogenesis (Ceci et al, 2021), to mention some. RNA secondary structures serve as binding platforms for the RNA-binding proteins, determine the assembly of ribonucleoprotein complexes and consequently affect gene expression (Lewis et al, 2017;Beltran et al, 2019;Duss et al, 2019;Rodgers & Woodson, 2019;Sanchez de Groot et al, 2019). This is well illustrated by riboswitches and internal ribosome entry sites (IRESs) that control prokaryotic and eukaryotic/viral gene expression, respectively (Jackson et al, 2010;Serganov & Nudler, 2013). These regulatory elements are usually located in the 59 UTRs but RNA structures within the coding sequences can also mediate translation. However, little is yet known about the dynamics and regulation of RNA structures in vivo.
G-quadruplexes (G4) are secondary structures found in both RNA and DNA that are formed by the stacking of at least two G-quartets, which are planar arrangements of four guanines connected by Hoogsten hydrogen bonds (Fay et al, 2017;Reznichenko et al, 2019). G4 conformation diversity depends on directionality (parallel, antiparallel, and hybrid), on the number of stacked G-quartets and on the length and sequence of the loops connecting the different strands, affecting stability and binding capacity to specific factors (Harris & Merrick, 2015;Tosoni et al, 2015;Fay et al, 2017). RNA G4 structures are linked to translation suppression when present in 59 UTR of the mRNA (Beaudoin & Perreault, 2010;Endoh & Sugimoto, 2016;Herdy et al, 2018) and to ribosome stalling when placed in the ORF Sugimoto, 2013, 2016;Endoh et al, 2013aEndoh et al, , 2013b. Despite being associated with important functions, the actual formation, stability, and activity of RNA G4s in vivo is still poorly understood and controversial and most of the predicted G4-forming sequences in RNAs do not form stable G4 structures in eukaryotes (Guo & Bartel, 2016). On the other hand, G4 structures in vitro using shorter RNA sequences show high thermostability (Arora & Maiti, 2009). RNA G4s are implied across various virus families, such as Flaviviridae (Fleming et al, 2016;Wang et al, 2016a;Jaubert et al, 2018), Herpesviridae (Murat et al, 2014), Filoviridae (Wang et al, 2016b), Paramyxoviridae (Majee et al, 2020), Retroviridae (Ruggiero et al, 2019), and Coronaviridae (Bezzi et al, 2021;Ji et al, 2021;Lavigne et al, 2021;Zhao et al, 2021), making them potential targets for drug development. In HIV-1, RNA G4s play an important role in the packaging of the virions (Lyonnais et al, 2003) and are part of the dimerisation process of the two copies of the HIV-1 RNA genome, facilitating recombination during reverse transcription (Marquet et al, 1991(Marquet et al, , 1994Piekna-Przybylska et al, 2013;Métifiot et al, 2014). In HCV, RNA G4s are implicated in the translation and replication of the genomic RNA (Wang et al, 2016a;Jaubert et al, 2018). EBNA1 and LANA1 are essential genome maintenance proteins of the EBV and the Kaposi's Sarcoma-associated herpesvirus (KSHV), respectively (Lan et al, 2004;Münz, 2015). Both are highly antigenic and to allow the viruses to escape immune surveillance the translation of their respective messenger RNAs is kept at the minimum to prevent the production of antigenic peptide substrates for the MHC-I pathway (Yin, 2003;Kwun et al, 2007). The synthesis of antigenic peptides for the MHC-I pathway is via non-canonical translation that is distinct from the canonical translation which generates full-length proteins, in line with the notion that fulllength proteins are a poor substrate for antigenic peptides (Cardinaud et al, 2004;Starck et al, 2008;Apcher et al, 2011;Wei et al, 2019;Yewdell et al, 2019). A glycine-alanine repeat (GAr) domain in EBNA1 is encoded by a guanine-rich sequence that forms G4 structures and binds nucleolin (NCL), inhibiting canonical and non-canonical translation initiation in cis to support viral immune evasion but how G4 structures support these different activities is not known (Yin, 2003;Apcher et al, 2009;Lista et al, 2017b;Martins et al, 2019). More recently, it was shown that the mRNA translation stress caused by the GAr domain activates the E2F1 oncogene and stimulates c-myc and ribosomal biogenesis (Gnanasundram et al, 2017). Hence, by suppressing its own synthesis, EBNA1 evades the immune system and promotes cell proliferation. The LANA1 of the KSHV also uses a cis-acting mechanism to evade immune surveillance (Murat et al, 2014) and as for EBNA1, both RNA G4 structures and repeat domains of LANA1 plays an important role in translation regulation for both full-length proteins and antigenic peptides production (Kwun et al, 2007;Apcher et al, 2009;Murat et al, 2014;Dabral et al, 2019). However, two main differences are worth being noticed when comparing these two functionally similar mRNAs and proteins, whereas the G4s in EBNA1 are located in the GArencoding domain, the G4s of LANA1 are spread throughout the message (Murat et al, 2014;Dabral et al, 2019). In addition, the peptide repeats of LANA1 and EBNA1 are different, although both are encoded by mRNA sequences predicted to form G4s.
We have used the properties of the EBNA1 and the LANA1 mRNAs to study the multifunctional aspects of G4 RNA structures. Each message has different G4 structures encoding different peptide sequences but share functional similarities in suppressing mRNA translation in cis, interacting with NCL and preventing mRNA export. The functions of their respective G4s can be separated by alterations in the flanking sequences and together with the observation that RNA G4s can reform in cells, these results illustrate how multifunctional and dynamic G4 structures act together to support viral immune evasion strategies. This sheds new light on some of the controversies surrounding the stability of G4 RNA structures and illustrates the importance of the context in which they are placed.

Results
G4 ligands PhenDC3 and PhenDH2 bind LANA1 mRNA G4 structures in vitro Using the QGRS mapper tool, and according to the work of Dabral et al (2019), the LANA1 message can form G4 structure at multiple points on the mRNA, but mostly in the region encoding the Central Repeat Domain of LANA1 ( Fig 1A). To confirm the binding of the G4 ligands we selected, namely, PhenDC3 and PhenDH2, on LANA1 RNA G4 structures, two quadruplex-prone G-rich short RNA sequences (LANA13 and LANA16 with QGRS scores of 21 and 18, respectively, Fig  1B) from the LANA1 Central Repeat (CR) domains, which have been already identified for their potential to form multiple quadruplex structures (Dabral et al, 2019), have been selected. First, to experimentally verify the RNA capacity to fold into quadruplex, their circular dichroism (CD) spectra were recorded (Fig 1B). In both cases, a positive peak at 265 nm together with a broad shoulder around 300 nm was observed. The CD spectra indicated the coexistence of different conformations in equilibrium, where the parallel conformation was the predominant. The RNA sequences exhibited a negative transition at 295 nm in the UV melting experiments which is characteristic of G4 unfolding ( Fig 1C) and allowed determining Tm values of 65-68°C and 45-48°C for LANA13 and LANA16, respectively. In presence of PhenDC3 or PhenDH2, the CD intensity is slightly decreased indicating that interaction is occurring between the G4 conformation and the ligands ( Fig 1B). Interestingly, when PhenDC3 was added before the annealing step, the shoulder at 300 nm was not observed (Fig S1), thereby suggesting that the ligand shifts the equilibrium towards the parallel conformation as has been recently reported for other G4 sequences (Aznauryan et al, 2021). Finally, we monitored the effects of PhenDH2 and PhenDC3 on the RNA sequences by CD melting. PhenDH2 and PhenDC3 induced on LANA16 a very strong stabilization (ΔTm = 40°C for PhenDH2 and >47°C with incomplete melting Tm > 95°C for PhenDC3) (Fig 1D). No complete melting of the G4 of LANA13 could be achieved in the presence of any ligand, indicating a very strong stabilization effect. Taken together, these data support the formation of G4 structures within the LANA1 CR domains and the possibility of targeting them with high affinity ligands such as PhenDC3 and PhenDH2.
G4 ligands affect LANA1 mRNA nuclear export, translation, and nucleolin binding The glycine-alanine repeat (GAr) domain of the EBV-encoded EBNA1 mediates a unique cis-acting suppression of its own synthesis via G4 RNA structures to minimise the production of EBNA1derived antigenic peptides for the MHC-1 pathway (Yin, 2003;Lista et al, 2017b). Similarly, the LANA1 of the KSHV also uses a cis-acting mechanism to evade the immune system via its CR domains (Kwun et al, 2007). Whereas the GAr-encoding message consists of GC-rich sequences encoding single alanine residues separated by two or three glycines, the CR domains of LANA1 are also GC rich, but each repeat region encodes for a different peptide motif (Kwun et al, 2007).
To know if LANA1 and EBNA1 are using similar molecular mechanisms involving RNA G4 structures to control mRNA translation, we first treated KSHV-carrying lymphoma B cells (BCP-1) and the EBV-carrying B-cell line B95.8 with 2 μM of the G4 ligands PhenDC3 or PhenDH2 for 24 h (Reznichenko et al, 2019). This resulted in an increase in the expression of respective proteins (Fig  2A). For EBNA1, this is linked to prevention of NCL binding to the RNA G4s by the G4 ligands (Lista et al, 2017a(Lista et al, , 2017b. Thus, this result shows that G4 structures in the LANA1 message, like in the EBNA1 message, are involved in translation suppression. We next expressed a FLAG-tagged LANA1 construct ( Fig 1A) in human H1299 carcinoma-derived cells and we observed a similar increase in protein levels after PhenDC3 and PhenDH2 treatment (Fig 2B), showing that the G4-mediated translation control is not dependent on cell line or on the presence of the whole viral genome, as for EBNA1 (Lista et al, 2017b). Unlike PhenDC3 and PhenDH2, another G4 ligand, the pyridostatin (PDS), is not able to interfere with the inhibition of LANA1 expression (Fig S2A), similarly to what was shown with EBNA1 (Lista et al, 2017b). We also inserted an eight amino acid-MHC-I antigenic peptide sequence (SL8) derived from the chicken ovalbumin gene (Ova) in the C-terminus of LANA1. The SL8 peptide is specifically recognised by CD8 + T cells (OT-1) when presented on the murine MHC-I (Kb) . After transient expression of LANA1 together with Kb in H1299 cells, we observed a twofold activation of CD8 + T cells after G4 ligand treatment, meaning that more SL8 antigenic peptide substrates were synthesised (Fig 2C), whereas the PDS is not able to interfere with the production of antigenic peptides for MCH-I pathway ( Fig S2B). As PhenDH2 is a more potent G4 ligand when it comes to EBNA1 mRNA compared with PhenDC3 (Reznichenko et al, 2019), we focused our investigation on PhenDH2 treatment. By using RNA FiSH, we observed that the LANA1 mRNA is located in the nucleus and that treatment with PhenDH2 promoted a cytoplasmic location ( Fig 2D). Furthermore, using the proximity ligation assay (PLA) we showed that PhenDH2 interferes with the LANA1 mRNAnucleolin (NCL) interaction in situ ( Fig 2E).
These results show that the EBV and KSHV have evolved a common strategy to exploit cis-acting G4 mRNA structures in the EBNA1 and LANA1 messages to prevent the production of antigenic peptide substrates for the MHC-1 pathway.
Deleting the LANA1 CR domain overcomes nuclear retention and stimulates synthesis of antigenic peptide substrates but does not affect the synthesis of full-length proteins or the interaction with nucleolin It has been suggested that the CR domains of the LANA1 account for mRNA translation control (Kwun et al, 2007) and we wanted to know if G4 activity in this region mediates the same functions as the EBNA1-encoded GAr sequence. When we deleted the CR domains (LANA1ΔCR), we observed no, or little, change in LANA1 protein expression, whereas we observed an average 60% increase in antigen presentation, indicating that different G4-forming domains in LANA1 control translation of antigenic peptide substrates and full-length proteins (Fig 3A-C). RNA FISH analysis detected most LANA1ΔCR messages in the cytoplasm ( Fig 3D). The PLA showed NCL-LANA1ΔCR mRNA interactions in the nucleus, even though the number of interactions were reduced when compared with fulllength LANA1 (Fig 3E). It should be pointed out that NCL is nuclear and preventing nuclear retention of the mRNAs could be sufficient to explain the differences in number of interactions.
Thus, G4 structures of the CR domain of the LANA1 message mediate nuclear retention and control of antigenic peptide synthesis but not full-length protein synthesis and NCL interaction.
G4s outside the CR domains control LANA1 protein expression and nucleolin binding The above results indicate that different LANA1 mRNA processing activities can be mediated by individual G4 structures throughout the LANA1 message. To test if NCL binding and the control of fulllength protein synthesis of the LANA1ΔCR construct is mediated by G4s predicted outside the CR domain, we treated cells expressing LANA1ΔCR with PhenDH2. This resulted in an average 50% increase in protein levels ( Fig 4A) and antigen presentation (Fig 4B). The treatment did not significantly affect LANA1ΔCR mRNA localisation ( Fig 4C) but prevented furthermore the interaction with NCL ( Fig 4D).
This confirms that the cis-mediated regulation of LANA1 mRNA translation is more complex as compared with the EBNA1 and involves G4s from different regions of the coding sequence and not just from the CR domains.
The position of the G4s within the coding sequence controls its activity It has been suggested that the interaction between NCL and G4s of EBNA1 mRNA is sufficient for suppressing synthesis of antigenic peptides and full-length proteins (Lista et al, 2017b), and it was surprising to see that deletion of the CR differentiates these two activities in the LANA1 mRNA. Therefore, we were interested to know if the G4 activities of the EBNA1 message can also be separated. To test this, we used constructs in which the GAr was fused to the 59 or the 39 end of the chicken ovalbumin (Ova) ORF, resulting in fusion proteins with the GAr in the N terminus or the C terminus (GAr-Ova and Ova-GAr, respectively) ( Fig 5A) (Yin, 2003). Both messages retained their nuclear localisation but the expression of the GAr-Ova fusion protein was considerable less, as compared with the Ova-GAr, whereas the RNA levels followed an opposite trend (Figs 5B and C and S3). When we isolated these RNAs from transiently transfected cells and compared their binding capacity to recombinant NCL, we observed that the GAr-Ova mRNA had a higher affinity to NCL compared with the Ova-GAr mRNA ( Fig 5D).
Hence, the location of the GAr G4s within the coding sequence of the message determines NCL binding and mRNA translation inhibitory activity but not nuclear retention. Of note, these results support the notion that NCL plays no role in G4-dependent mRNA export.
Flanking UTR sequences affect LANA1 and EBNA1 G4s differently The GAr-Ova and the Ova-GAr reporter constructs showed that the localisation of the G4-containing translational inhibitory sequence within the coding sequence is important for some, but not all, G4related activities. We next wanted to know if changes in the noncoding sequences can also affect G4 functions. We introduced RNA structures in the form of the c-myc and HCV IRESs in the 59 UTR of the GAr-Ova construct. We could confirm that the c-myc-GAr-Ova construct and c-myc-Ova are equally efficiently translated (Apcher et al, 2009). The fusion of the HCV IRES (HCV-GAr-Ova) instead further suppressed expression of GAr-Ova as well as Ova alone (HCV-Ova) ( Fig 6A)  . When the two IRESs were individually fused to the LANA1 message, they both stimulated synthesis of full-length proteins ( Fig 6B). Interestingly, although only the HCV IRES suppressed synthesis of GAr-Ova and Ova proteins, the presence of either IRES in the 59 UTRs resulted in an increase in the synthesis of antigenic peptide substrates from GAr-Ova and LANA1 messages (Fig 6C and D). Hence, although the HCV IRES suppressed expression of full-length GAr-Ova and Ova, it stimulated synthesis of antigenic peptide substrates from both GAr-Ova and LANA1 messages. Both IRESs also prevented NCL binding to GAr-encoding and LANA1 mRNAs, but only the HCV IRES promoted mRNA nuclear export (Figs 6E and S4-S6). Fusing the c-myc and the HCV structures to the 39 UTR of LANA1 had little effects on G4 activities (Fig S7A and B).
This demonstrates that the different functions of the G4s within the messages can be separated by altering the 59 UTR. These data further underline that the synthesis of full-length proteins and antigenic peptides are derived from two independent mRNA translation events.

Discussion
We have compared four different activities related to G4 structures within the coding sequences of the KSHV-and EBV-encoded LANA1 and EBNA1, respectively. These proteins play similar key roles in maintaining viral genome integrity and both are expressed in all virus-infected cells, including cancerous ones, making their respective expression interesting therapeutic targets for virusassociated diseases (Ballestas, 1999;Ballestas & Kaye, 2001;Ciufo et al, 2001;Kwun et al, 2007;Münz, 2015). Both viruses need to ensure that the production of peptide substrates for the MHC-I pathway is kept at a minimum to evade the immune system (Kwun et al, 2007;Murat et al, 2014). To achieve this, they have evolved cis-acting mechanisms to suppress translation of the LANA1 and EBNA1 messages. Even though the peptide sequences mediating this effect are not similar (Kwun et al, 2007), the concept to control mRNA processing and translation to achieve immune evasion is. This suggests that controlling their own rate of translation might be a common strategy whereby, at least latent, viral messages evade the immune system. In agreement with this hypothesis is the observation that viruses causing persistent infections in Metazoa hosts are significantly enriched with putative G-quadruplex forming sequences (PQS), whereas viruses causing acute infections are significantly depleted in PQS (Bohálová et al, 2021).
Treatment with the G4 ligand PhenDH2 resulted in an increase in protein expression, antigen presentation, nuclear export, and loss of NCL binding in the context of EBNA1 and LANA1, indicating that these four events are indeed all G4 dependent. The G4s of EBNA1 mRNA are mostly clustered within the gly-ala repeat GAr-encoding sequence (Murat et al, 2014), whereas the G4 activities of LANA1 are spread throughout the message. The G4s in the CRs of LANA1 are determinant for nuclear mRNA retention and suppression of antigenic peptide substrates, whereas G4s outside the CR domains are important for canonical translation control and nucleolin interaction. Both CR and non-CR-related G4s play roles in antigen production. The fact that the CR domains play an important role in   controlling the synthesis of antigenic peptides is in line with previous observations (Kwun et al, 2007). However, the results presented here do not support the CR domains being the sole responsible for controlling synthesis of antigenic peptides and fulllength proteins from the LANA1 mRNA. Nevertheless, we did not analyse the role of the individual LANA1 repeat domains and together with the fact that flanking sequences play a role in determining G4 activities this can help in explaining this discrepancy.
An important conclusion from this study is that different G4 activities within an mRNA can be differentiated by altering flanking sequences, illustrating the dynamic nature of G4 RNA structures. For example, moving the GAr sequence from the 59 towards the 39 end of the coding sequence reduces its translation inhibitory capacity and NCL binding without affecting nuclear retention. Introducing the structured RNAs of the HCV or the c-myc IRESs affect LANA1 and GAr mRNA translation and localisation differently. The HCV IRES further suppressed synthesis of GAr-carrying mRNAs, whereas it instead stimulated LANA1 expression and released the nuclear retention of both mRNAs. The c-myc IRES, on the other hand, does not affect RNA localisation but induces translation of both messages . Also G4-mediated regulation of canonical translation producing full-length proteins and non-canonical translation linked to antigenic peptides synthesis could be separated by introducing the HCV IRES in the 59 of the GAr.
Previous works show that RNA G4 structures can fold, unfold and refold in cellulo (Biffi et al, 2014;Chen et al, 2018;Yang et al, 2018). The EBNA1 message does not contain introns but placing the GAr in the context of a gene prevents NCL access in cellulo. However, placing the GAr in this context does not affect the G4 structure and RNAs isolated from cells interact with recombinant NCL . The interaction between the GAr derived from a spliced construct and NCL was observed in the cytoplasm when the nuclear localisation signal of NCL was removed . As the spliced message is subjected to the pioneer round of translation, the GAr G4s would have had to refold to allow NCL interaction in the cytoplasm. These observations indicate that the function of G4s in the regulation of mRNA processing is strongly influenced by events taking place during the mRNA maturation process and that they can act both in the nucleus as well as in the cytoplasm. In this context, G4s multifunctionality might be affected by any change impacting the nature of the ribonucleoparticles. In fact, several RNA binding proteins are reported to be involved in the folding and unfolding of G4s RNA for a specific regulation event (Wolfe et al, 2014;Herviou et al, 2020;Vannutelli et al, 2020).
The role, and actual in vivo existence, of G4 RNA structures is controversial (Guo & Bartel, 2016;Waldron et al, 2018;Dumas et al, 2021). Our data support a model in which G4 structures form dynamic multifunctional units, showing similarity to the concept of intrinsic disordered proteins. The allosteric conformation of intrinsic disordered proteins is regulated by post-translational modifications and/or ligand binding that alter their interactomes and their functions (Habchi et al, 2014;Borkosky et al, 2017). The RNA G4s could follow a similar scheme, in which ligand binding, or RNA modifications, could affect the structure of the G4s and alter their activity. In the examples described here, the activities of the LANA1 and EBNA1 G4s can be disrupted by chemical ligands, by moving their position or by changing flanking sequences.
It is conceivable that the dynamic multifunctional roles of these herpes viral RNA G4s are not unique but reflect a more common nature of regulated RNA structures within coding sequences. In the case of the p53 mRNA, a structured region in the 59 of the coding sequence is folded during genotoxic stress so that it binds to MDM2 and stimulates p53 synthesis. The RNA folding is prevented by a single cancer-derived synonymous mutation, illustrating how single nucleotides can affect the folding of an mRNA and its interaction with regulatory proteins (Candeias et al, 2008;Naski et al, 2009). In line with this, modifications of mRNAs can result in structural changes and, consequently, affect their functions and interactions with cellular factors (Lewis et al, 2017). For example, the binding of hnRNPC to cryptic U-tract sequences can be made available via N6-methylation on adenosine residues (Liu et al, 2015).
Treatment with chemical G4 ligands PhenDC3 or PhenDH2 disrupts GAr and LANA1 G4s functions. However, the PDS G4 ligand stimulates the inhibition of EBNA1 synthesis in vitro (Murat et al, 2014) but not in vivo and it does not affect NCL binding (Lista et al, 2017b;Martins et al, 2018). Such differences are also observed for LANA1. Dabral et al (2019) report that the G4-stabilising ligand TMPyP4 reduces the translation of LANA1 mRNA, but we show here that PhenDC3 or PhenDH2 treatments increase LANA1 protein expression and the presentation of LANA1-derived antigens. It is, thus, likely that different G4 ligands affect RNA structures differently by being able to either stabilise or destabilise them and/or to interfere with their ability to represent binding platforms for various RNAbinding factors. Considering the dynamic function of the herpes viral G4s, this suggests that G4 structures and activities can be specifically targeted for therapeutic intervention and that screening of G4-targeting compounds should be carried out in an in vivo setting with an intact message to obtain a specific activity.

Materials and Methods
Annealing of RNA sequences for CD spectroscopy The G-rich RNA oligonucleotide (ON) sequences LANA13 (59-AGGAGGUGGAGGA), genomic loci 2563, G-score 21, and LANA16 (59-AGGAGCAGGAGGUGGA, genomic loci 2635, G-score 18) (Dabral et al, 2019) were purchased in Eurogentec as lyophilized solid after HPLC separation. They were reconstituted in DEPC water, and their concentration calculated by measuring the UV absorption at 260 nm on a Hitachi 2900 spectrometer. RNA ON buffered solutions (5 μM ON; Li Cacodylate 10 mM, KCl 100 mM) were annealed in a thermo-block by heating 2 min at 95°C and let it cool until reaching room temperature.

CD footprint
The CD of the previously annealed ON sequences (LANA13 and LANA16) were measured alone or in the presence of 2 M equivalents of PhenDC3 or PhenDH2 in a Jasco J-1500 CD spectrometer using a quartz cuvette of 0.5-cm path length. The parameters were four accumulations, CD scale 200 mdeg/0.1 dOD, scanning speed 100 nm/min. The spectra shown were mathematically processed using the Savitzky-Golay method with 20 points of window (del Villar-Guerra et al, 2017).

CD melting
The CD melting of the ON sequences containing (LANA13 and LANA16), alone or in the presence of ligand, were monitored using a Jasco J-1500 CD spectrometer equipped with external temperature water-circuit control. The CD was recorded at 262 and 295 nm every 0.2°C change, when heating the samples from 20°C to 95°C at a speed of 0.2°C/min.

UV melting
The UV melting of the ON sequences containing (LANA13 and LANA16) in absence of ligand were monitored using a Spectro UV Cary-300 equipped with external temperature water-circuit control. The UV was recorded at 295 nm when heating the samples from 20°C to 95°C at a speed of 0.2°C/min.
All expression plasmids were constructed using the pcDNA3 vector and amplified in the Escherichia coli DH5α strain. The constructs named Ova, GAr-Ova and Ova-GAr encode the following proteins, respectively: Ovalbumin without the first 50 amino acids, the same protein with the GAr domain from EBNA1 fused to its N terminus or C terminus (Yin, 2003).
The pA3F-LANA1 plasmid carrying the Flag-tagged ORF73 of KSHV in pcDNA3 vector, which encodes wild-type LANA1 protein, was a kind gift from Pr. Blondel (University of Brest). LANA1-SL8 construct was created by inserting the SL8 (SIINFEKL peptide from chicken ovalbumin) coding sequence after the C-terminal domain of LANA1. LANA1ΔCR-SL8 construct was generated using the QuikChange Site-Directed Mutagenesis System (Stratagene) and the construct LANA1-SL8 as template.
The plasmids c-myc_IRES_Ova, c-myc_IRES_GAr-Ova, HCV_IRES_Ova, and HCV_IRES_GAr-Ova encode Ovalbumin or a fusion of the GAr domain to the N terminus of ovalbumin, with either the c-myc IRES or the HCV IRES in their 59 UTR (Apcher et al, 2009). HCV and c-myc IRES were amplified from these constructions and fused to the 59 UTR or 39 UTR of LANA1-SL8 plasmid to produce the 59 c-myc-IRES-LANA1, 39 c-myc-IRES-LANA1, 59 HCV-IRES-LANA1, and 39 HCV-IRES-LANA1 constructs. The list of primers, cloning method, and resulting plasmids is provided in Table S1.

Transfection and drug treatment
H1299 cells were seeded in six-well plates (7 × 10 4 cells/well) and transient transfections were carried out 20-24 h later using the Genejuice reagent (Merck Bioscience) according to the manufacturer's protocol. For cell treatments with PhenDC3, PhenDH2, and PDS (Lista et al, 2017b;Reznichenko et al, 2019), cells were incubated with 2 μM of drug 24 h after transfection. Drug stock solutions were prepared in DMSO (Euromedex).
Predicted molecular weight for the full-length LANA1 is 135 kD; however, as it was previously described, the LANA1 full-length protein migrate at 234 kD in SDS-PAGE (Rainbow et al, 1997). In addition, the LANA1ΔCR product is predicted to have a molecular weight of 61 kD, but migration with SDS-PAGE resulted in an observed band of 100 kD.

RNA FiSH
H1299 cells were seeded in 24-well plates (2 × 10 4 cells/well) and transient transfections were carried out 20-24 h later using the Genejuice reagent (Merck Bioscience) according to the manufacturer's protocol. 24 h after transfection or after drug treatment, the cells were briefly washed with ice-cold PBS, fixed with 4% PFA during 20 min at room temperature, and washed again with PBS. Cells were then incubated in 70% ethanol for 4-24 h at 4°C, after intermediate dehydrating steps using 30% and 50% ethanol. For rehydration, the cells were incubated in 50% and 30% ethanol and further washed with PBS. Subsequently, cells were permeabilised with PBS 0.4% Triton 0.05% CHAPS for 5 min at room temperature. After three PBS washes, samples were pre-treated with two subsequent incubations in FiSH wash buffer (10% formamide and 2X SSC in ddH 2 O) for 10 min and then in FiSH hybridisation buffer (10% formamide, 2X SSC, 2 mg/ml BSA, 0.2 mg/ml E. coli tRNA, 0.2 mg/ml sheared salmon sperm DNA) for 30 min at room temperature. Coverslips were then incubated overnight in a wet chamber at 37°C in FiSH hybridisation buffer supplemented with 10% dextran sulphate and 100 nM of FiSH Stellaris probes targeting Ovalbumin or LANA1 mRNAs (Biosearch Technologies). Coverslips were washed twice 20 min in FiSH hybridisation buffer and 5 min in FiSH Wash buffer and subsequently stained with DAPI. Samples were mounted using Dako mounting solution (Dako) and observed with a LSM 800 (Zeiss) confocal laser microscope. Images were obtained using Zen software (Zeiss) and the Costes colocalisation factor between nuclei (DAPI channel) and targeted mRNAs (Cy3 channel), called Correlation_Costes_DAPI_Cy3, were obtained using the software CellProfiler (McQuin et al, 2018), and later used for statistical analysis.

Proximity ligation assay (RNA-protein)
Cells were cultured, fixed, permeabilised, and submitted to a prehybridisation step as described above. For target-mRNAs hybridisations, DNA probes were denatured for 5 min at 80°C and diluted in hybridisation buffer at a concentration of 50 ng of probes per sample. The probes 59 GCAGCAGACTACACCTCCACACTCACC-biotin 39 and 59-CTGCTTCATTGATTTCTGCATGTGCTGCATGGACAGCTTGAAAAA-digoxigenin 39 were designed to target LANA1 and Ovalbumin mRNAs, respectively. Samples were overnight incubated with the denatured DNA probes in a wet chamber at 37°C. Samples were then washed in hybridisation buffer, FiSH wash buffer, and PBS and saturated with PBS 3% BSA 0.1% saponin for 1 h at room temperature. Primary antibodies were diluted in the saturation solution, kept for 30 min at room temperature and then overnight incubated with tested samples at 4°C in a wet chamber. The following antibodies were used to perform the PLA: anti-Digoxigenin mouse monoclonal antibody (clone DI-22, D8156; Sigma-Aldrich), anti-Biotin mouse monoclonal (clone BN-34, B7653; Sigma-Aldrich), and anti-NCL rabbit polyclonal antibody (ab22758; Abcam). Afterwards, PLA was performed using Duolink PLA kit and plus/minus probes (Sigma-Aldrich) following the manufacturer's instructions. Coverslips were finally mounted using Dako mounting buffer (Dako) after nuclear staining with DAPI and observed using an LSM 800 confocal laser microscope. Images were obtained using the Zen software and analysed using Fiji/ImageJ.

Antigen presentation
Naïve OVA257-264-specific CD8 + T-cells were isolated from the peripheral and mesenteric lymph nodes of OT-I-mice using the CD8 + -isolation kit (Miltenyi Biotec). Subsequently, 4 × 10 5 CD8 + T-cells were co-cultured with 10 5 H1299 cells previously transfected with the indicated constructs and a mouse Kb expression vector (a kind gift from C Watts, University of Dundee) and treated or not with the tested drugs. Cells were cultured in RMPI 1640 (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine, 100 U/ml streptomycin and 100 U/ml penicillin (Invitrogen), 10% FBS, 0.05 mM 2-mercaptoethanol, and 5 mM Hepes (Sigma-Aldrich) for 3 d at 37°C with 5% CO 2 . Supernatants were collected from the co-cultures and IL-2 levels were measured by ELISA using ELISA MAX Standard kit (BioLegend) following the manufacturer's protocol. Signals were measured using a FLUOstar Optima (BMG Labtech) and data were analysed using the software Optima Control v2.20R2.

Statistical analysis
Data were analysed by unpaired Mann-Whitney's test or t test on GraphPad Prism 9. For Western blot, PLA dot counts, and qPCR analyses, represented data are the mean and the SD of a minimum of three independent experiments. For RNA co-IP analyses, represented data are the mean and the standard error of a minimum of three independent experiments. For FiSH analysis, data represented on graphs are the median value, 25% and 75% quartiles delimiting the interquartile range, the maximum and minimum values and the outliers. In all cases, P > 0.1234 (ns), P < 0.0332 (*), P < 0.0021(**), and P < 0.0002(***). was funded by Université de Paris and the Fondation pour la Recherche Médicale (grant number FDT202001010912). This work was partially supported by European Regional Development Fund (ENOCH, CZ.02.1.01/0.0/0.0/ 16_019/0000868), MH CZ-DRO (MMCI, 00209805), Cancerforskningsfonden Norr, Cancerfonden (160598), Vetenskapsradet, and by the International Centre for Cancer Vaccine Science within the International Research Agendas program of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund.