Microglia show differential transcriptomic response to Aβ peptide aggregates ex vivo and in vivo

Exogenous amyloid beta peptides cause distinct transcriptomic responses in microglia cultures that are not fully replicated in an amyloid mouse model or identified microglial subpopulations in vivo.

Thank you for submitting your revised manuscript entitled "Microglia show differential transcriptomic response to Aβ peptide aggregates ex vivo and in vivo". We would be happy to publish your paper in Life Science Alliance (LSA) pending minor text-based revisions mentioned below and final revisions necessary to meet our formatting guidelines.
For a brief overview, the manuscript was previously reviewed at a LSA partner journal, and the authors transferred the manuscript, along with the referee reports to LSA. At LSA, the manuscript and reviewer reports were assessed by both in-house editors and academic experts, who agreed that the dataset provided in the study was high quality and would be a valuable resource for the research community. The authors responses to the 2 points of concerns raised by the LSA editors (sent with the decision letter from the partner journal) were also sufficiently addressed by the authors. Thus, we would like to invite you to submit a final revision of this manuscript that includes the following minor edits: + We understand the argument that the authors have made in response to the concern from Rev 3 pt 2 about the 'cleanliness' of the oligomeric vs fibrillar preparations. We encourage the authors to discuss the caveats about this experiment in the manuscript, similar to what they included in the response + the minor revisions requested by the reviewers should be addressed in the revised manuscript Along with the points listed above, we also encourage the authors to edit the following to meet the journal's formatting guidelines: -please add a Summary Blurb/Alternate Abstract in our system -please add Keywords and a Category for your manuscript in our system -please add ORCID ID for secondary corresponding author-they should have received instructions on how to do so -please consult our manuscript preparation guidelines https://www.life-sciencealliance.org/manuscript-prep and make sure your manuscript sections are in the correct order -please add your main, supplementary figure, and table legends to the main manuscript text after the references section -please make sure the manuscript sections are aligned in accordance with LSA's formatting guidelines: please separate the Figure legends and Supplemental Figure legends into separate sections -please add an Author Contributions section to your main manuscript text -please add a conflict of interest statement to your main manuscript text -we encourage you to revise the figure legends for figures 7 and S1 such that the figure panels are introduced in an alphabetical order -there is a callout for Figure S3A and B although there is no legend for it nor the actual figure has been provided -please add callouts for Figures 7B, C, and 8F-H to your main manuscript text -please provide higher resolution higher quality images for the blots shown in Figure 1 If you are planning a press release on your work, please inform us immediately to allow informing our production team and scheduling a release date.
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Thank you for this interesting contribution, we look forward to publishing your paper in Life Science Alliance.