Genetic and molecular characterization of multicomponent resistance of Pseudomonas against allicin

A pseudomonad isolated from garlic has multiple copies of genes on three genomic islands that confer resistance to allicin, a secondary plant product and defense substance that causes disulfide stress.

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Sincerely, Andrea Leibfried, PhD Executive Editor Life Science Alliance Meyerhofstr. 1 69117 Heidelberg, Germany t +49 6221 8891 502 e a.leibfried@life-science-alliance.org www.life-science-alliance.org This manuscript provides genetic and molecular characterization of the allicin resistance mechanism of the garlic-adapted bacterium Pseudomonas fluorescens. There is a high degree of novelty in this well-written, experimentally solid manuscript, which with minor changes merits publication as is.
Minor errors: 1) mis-use of semicolons where commas are needed (line 50 after close-parenthesis and line 490) 2) The statement on lines 450-453 is incorrect "Although onion does not produce allicin, it has many sulfur containing redox-active compounds which may be involved in defense (Imai et al. 2002), for example diallyl tetrasulfane, which was recently shown to S-thioallylate a similar spectrum of proteins to allicin in Bacillus subtilis (Chi et al. 2019)." While onion does contain various sulfur compounds, there are no allyl compounds present so "diallyl tetrasulfane" cannot be correct.
3) The referencing style is inconsistent with some titles containing all first letters capitalized and some not. Shouldn't all botanical terms in titles be italicized as is done in the manuscript text?
Apart from these minor errors, this is an excellent manuscript meriting publication without change.
Reviewer #2 (Comments to the Authors (Required)): The manuscript by Borlinghaus et al. describes a comprehensive study of a recently discovered pseudomonad strain resistant to the highly aggressive redox modulator allicin. The authors demonstrate that this resistance is most likely acquired by horizontal gene transfer and results from the interplay of several proteins and enzymes, of which about half are redox related. Overall this is a very interesting manuscript, nicely written and presented, and of considerably significance for the field of redox biology.
Some parts, such as the section on pages 10 and 11, are very exhaustive. Then again, I am not an expert in gene transfer and such details may be of interest to colleagues active in this field. Thank you for submitting your manuscript entitled "Genetic and molecular characterization of a multi-component resistance mechanism of a pseudomonad against allicin". Your work has now been peer-reviewed by two experts and I attach their reports below.
As you will see, the reviewers appreciate your data and only have minor suggestions for improvement. We would thus like to invite you to submit a revised version of your manuscript to us. While responding to the specific concerns of the two reviewer, please also pay attention to the following:

Reviewer #1 (Comments to the Authors (Required)):
This manuscript provides genetic and molecular characterization of the allicin resistance mechanism of the garlic-adapted bacterium Pseudomonas fluorescens. There is a high degree of novelty in this well-written, experimentally solid manuscript, which with minor changes merits publication as is. 2) The statement on lines 450-453 is incorrect "Although onion does not produce allicin, it has many sulfur containing redox-active compounds which may be involved in defense (Imai et al. 2002), for example diallyl tetrasulfane, which was recently shown to S-thioallylate a similar spectrum of proteins to allicin in Bacillus subtilis (Chi et al. 2019)." While onion does contain various sulfur compounds, there are no allyl compounds present so "diallyl tetrasulfane" cannot be correct.

An embarrassing error, thank you for spotting it. We have revised this statement.
3) The referencing style is inconsistent with some titles containing all first letters capitalized and some not. Shouldn't all botanical terms in titles be italicized as is done in the manuscript text?

Latin binomials have been italicized throughout, but the '1 st letter capitals vs. lower case first letters' reflects the different citation formats in the original Journal citations and we do not feel at liberty to change that.
Apart from these minor errors, this is an excellent manuscript meriting publication without change.
Reviewer #2 (Comments to the Authors (Required)): The manuscript by Borlinghaus et al. describes a comprehensive study of a recently discovered pseudomonad strain resistant to the highly aggressive redox modulator allicin. The authors demonstrate that this resistance is most likely acquired by horizontal gene transfer and results from the interplay of several proteins and enzymes, of which about half are redox related. Overall this is a very interesting manuscript, nicely written and presented, and of considerably significance for the field of redox biology.
Some parts, such as the section on pages 10 and 11, are very exhaustive. Then again, I am not an expert in gene transfer and such details may be of interest to colleagues active in this field. Thank you for submitting your Research Article entitled "Genetic and molecular characterization of multi-component resistance of Pseudomonas against allicin". I appreciate the introduced changes and it is a pleasure to let you know that your manuscript is now accepted for publication in Life Science Alliance. Congratulations on this interesting work.
The final published version of your manuscript will be deposited by us to PubMed Central upon online publication.
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You can contact the journal office with any questions, contact@life-science-alliance.org Again, congratulations on a very nice paper. I hope you found the review process to be constructive and are pleased with how the manuscript was handled editorially. We look forward to future exciting submissions from your lab.