Newest Articles
Specificity for deubiquitination of monoubiquitinated FANCD2 is driven by the N-terminus of USP1Deubiquitination of FANCD2, FANCI, and PCNA by USP1 is essential for DNA repair signalling. Reconstitution of the system reveals that USP1-mediated specificity towards K561 of FANCD2 is directed by a unique sequence at USP1's N-terminus.
Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA librariesHigh-throughput inverse toeprinting identifies peptide-encoding transcripts that induce ribosome stalling and allows the systematic analysis of sequence-dependent translational events.
Loss of HDAC11 ameliorates clinical symptoms in a multiple sclerosis mouse modelIn an animal model for multiple sclerosis, the absence of HDAC11 reduces clinical severity, spinal cord demyelination, and immune cell infiltration, suggesting that HDAC11 is a promising target for MS treatment.
Secretory granule protein chromogranin B (CHGB) forms an anion channel in membranesThe CHGB subfamily of secretory granule proteins forms a new family of anion-selective channels by interacting with membranes via two amphipathic α-helices. The channel exhibits higher anion selectivity, larger conductance, higher DIDS-binding affinity, and higher Cl− sensitivity than other known anion channels.
Interaction modulation through arrays of clustered methyl-arginine protein modificationsExtensively modifiable arrays of clustered arginines in a large set of human proteins function as regulatory protein interaction platforms. Quantitative immunoprecipitation assays defined two distinct cumulative binding mechanisms operating across an array of 18 methyl-arginine motifs in SYNCRIP.
S-phase transcriptional buffering quantified on two different promotersTranscriptional buffering enforced during DNA replication shows that histone acetylation governs the homeostasis process and can also restrict promoters from reaching maximum transcriptional potential
CARM1 methylates MED12 to regulate its RNA-binding abilityCARM1 methylates MED12 at arginine 1899 to generate a TDRD3 binding site, which in turn regulates the ability of mediator to interact with activating ncRNAs and modulate gene expression.
Identification of Plasmodium GAPDH epitopes for generation of antibodies that inhibit malaria infectionThis study reports on the identification of two Plasmodium GAPDH epitope peptides that are responsible for sporozoite–Kupffer cell interaction and act as antigens against malaria infection.
Transposon silencing in the Drosophila female germline is essential for genome stability in progeny embryosSuppression of transposons by the Piwi-interacting RNA biogenesis factor Vasa in the supporting nurse cells is essential to prevent their accumulation in the developing oocyte, ensuring proper Drosophila embryonic development.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethalityMouse embryos with an ablated ability of integrins to bind laminins are still able to form basement membranes, but die just after implantation because of deficient extraembryonic development.
