Newest Articles
- Passive diffusion accounts for the majority of intracellular nanovesicle transport
In contrast to large vesicles and organelles that are moved by motors inside cells, a detailed analysis of intracellular vesicle mobility reveals that passive diffusion is the main mode of small vesicle transport.
- The coenzyme A precursor pantethine enhances antitumor immunity in sarcoma
VitB5 level becomes limiting in sarcomas. It is regulated by the pantetheinase activity of VNN1. VNN1 expression in sarcomas is associated with better prognosis and immune infiltration. In mouse, complementation with pantethine a vitB5 precursor boosts antitumor immunity.
- Structural insights into the complex of oncogenic KRas4BG12V and Rgl2, a RalA/B activator
The work reports the KRas4BG12V oncogenic mutation alters the binding kinetics with Rgl2, a RalA/B activator, and the V12 resides at the KRas4B(G12V)•Rgl2 complex interface in the crystal structure.
- Assessing MAPPs assay as a tool to predict the immunogenicity potential of protein therapeutics
This work highlights the use of streptavidin magnetic beads as a valuable approach for the immunoaffinity purification of mass spectrometry MHCII-eluted ligands (MAPPs) and their practical application in assessing the drug-induced immunogenicity risk of novel biotherapeutics.
- High-resolution analysis of bound Ca2+ in neurons and synapses
The amount of bound Ca2+ in neurons correlates significantly to the intensity of markers for GABAergic pre-synapse and glutamatergic post-synapses, and negatively to pre-synaptic activity.
- SBDSR126T rescues survival of sbds−/− zebrafish in a dose-dependent manner independently of Tp53
We created a transgenic line expressing the disease-associated mutation SBDSR126T in the sbds KO zebrafish background. We demonstrated that amount of SBDSR126T is important for development and tp53 pathway activation. Tp53M214K did not rescue neutropenia or survival in the sbds-null zebrafish.
- Identification of biomarkers, pathways, and therapeutic targets for EGFR–TKI resistance in NSCLC
The exploration of the hub genes related to EGFR–TKI resistance in NSCLC through bioinformatics technologies can improve the understanding of the mechanisms of EGFR–TKI resistance and provide novel insights into therapeutics.
- The highly improved genome of Ixodes scapularis with X and Y pseudochromosomes
Ixodes scapularis Gulia-Nuss (IscGN) genome assembly and gene set were obtained using DNA from eggs and male and female adult ticks. By combining Hi-C, PacBio HiFi, and Illumina sequencing technologies, we assembled 13 pseudoautosomes and sex chromosomes: X and Y.
- Synergistic effect of antagonists to KRas4B/PDE6 molecular complex in pancreatic cancer
C14 and P8 combo reveals potent synergy in PDAC models, promising safer, effective therapy than gemcitabine.
- Fn14 promotes myoblast fusion during regenerative myogenesis
Genetic and molecular approaches demonstrate that Fn14 receptor–mediated signaling improves myoblast fusion during skeletal muscle regeneration in a cell-autonomous manner.