Newest Articles
- Interaction hub critical for telomerase recruitment and primer-template handling for catalysis
By deleting, mutating, and adding back parts of the telomerase enzyme that extends chromosome ends, we uncover features important for its proper functioning in the cell.
- Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii
The OTU deubiquitinase family is expanded in Toxoplasma parasites, and members show preferences for Lys6-, Lys11-, Lys48-, and Lys63-linked ubiquitin chains, and additional specificity for NEDD8. AlphaFold-guided structural analysis reveals cryptic ubiquitin-binding domains with functional importance.
- Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes
Filopodia-like protrusions sent by the somatic cells to the oocyte play a role in the structural integrity of the germline–somatic complex, and regulate gene expression in the oocyte and thus its developmental potential and chances to produce a viable embryo.
- Cell death and barrier disruption by clinically used iodine concentrations
Clinically used concentrations of povidone-iodine (PVP-I) are toxic to eukaryotic cells by attacking the lipid bilayer and tight junctions with fast kinetics and urge the reappraisal of PVP-I use.
- H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra
H4K20me1 modification by SETD8 methyltransferase is typically associated with repression. In Hydra, it is associated with activation machinery locally at Wnt-responsive promoters indicating its dual role in transcriptional regulation.
- Structure-based design and characterization of Parkin-activating mutations
Parkin, an E3 ubiquitin ligase involved in Parkinson’s disease, is inactive in the basal state and is activated by PINK1 to mediate mitophagy. Here, we characterized 31 mutations and discovered three that activate Parkin and rescue loss of PINK1 phosphorylation.
- Potency assessment of IFNγ-producing SARS-CoV-2-specific T cells from COVID-19 convalescent subjects
SARS-CoV-2-specific T-cells from convalescent donors present phenotypical and functional characteristics that make them a suitable candidate for a future development of an advanced cellular therapy.
- MicroRNAs in tear fluids predict underlying molecular changes associated with Alzheimer’s disease
This study demonstrated the translational potential of deregulated tear fluid miRNAs associated with amyloid beta production and proinflammation in a transgenic AD mouse model.
- Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
The combination of thermal proteome profiling and pulsed SILAC labelling shows that mitochondrial dysfunction imprints a characteristic pattern on the abundance and thermal stability of the proteome.
- A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenates
This novel method can be used in clinical samples to determine CV function in diseases, with the added benefit of being able to use frozen samples in a high-throughput manner and to explore ATP hydrolysis as a drug target for disease treatment.