Newest Articles
- Brain IGF-I regulates LTP, spatial memory, and sexual dimorphic behavior
A lack of brain-synthesized IGF-I almost completely abrogates hippocampal long-term potentiation (LTP), alters spatial memory and sex-dependent behavior, and produces major changes in the mouse hippocampal proteome.
- Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesions
Using fluorescence polarisation microscopy and computational modeling, we find that FA molecules exhibit ECM dependent orientational co-alignment or order. This molecular organizational property in addition to nanoscale localization may explain precise and sensitive decoding of ECM cues.
- The C-terminal tail of CSNAP attenuates the CSN complex
The study demonstrates inhibition of the COP9 signalosome (CSN) complex by a peptide derived from CSNAP, the smallest CSN subunit. The peptide displaces the endogenous CSNAP subunit from the complex, leading to a CSNAP null phenotype that attenuates CSN activity.
- The pyruvate dehydrogenase complex regulates mitophagic trafficking and protein phosphorylation
Mutations in the PDC affect the phosphorylation and mitophagic trafficking of matrix proteins, through the novel regulation of associated kinases and a phosphatase. We suggest that this occurs by the direct allosteric regulation of the phosphatase and kinases by the PDC.
- Transcriptome-wide RNA binding analysis of C9orf72 poly(PR) dipeptides
C9orf72 poly(PR) binds to 558 RNAs in human cells, and the sequence GAAGA is enriched at its binding sites. Poly(PR) has nanomolar affinity for GAAGA RNA, and this RNA induces poly(PR) phase separation.
- Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
A cohort of low-abundance transmembrane proteins concentrated at the nuclear envelope has been identified, predicting diverse new functions for this membrane system.
- Lsd1 safeguards T-cell development via suppressing endogenous retroelements and interferon responses
Bulk and single-cell RNA-seq, combined with ChIP-seq analysis, reveal that the loss of Lsd1 leads to the aberrant depression of endogenous retroelements and subsequent IFN response signaling in thymocytes, which are possible causes of impaired early T cell development.
- B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses
Daratumumab is an anti-CD38–targeting antibody that depletes plasma cells. This study shows that daratumumab also disturbs humoral immune responses beyond depletion only and may be used as therapeutic in B cell–mediated autoimmune diseases.
- Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20
This work characterizes the transcriptional and epigenetic control of memory CD8 T cell differentiation by the BTB-ZF family transcription factor Zbtb20 using single cell RNA and ATAC sequencing and identifies direct genomic targets of Zbtb20 using CUT&RUN.
- Differential roles of FOXC2 in the trabecular meshwork and Schlemm’s canal in glaucomatous pathology
FOXC2 is required in the Schlemm’s canal endothelium and neural crest-derived trabecular meshwork cells for the morphogenesis and maintenance of Schlemm’s canal.