Newest Articles
Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editorA chimeric fusion of T7 RNAP and deaminase edits the DNA under the T7 promoter in plant cells. It directs the continuous synthetic evolution of OsALS to produce variants with herbicide resistance.
Timapiprant, a prostaglandin D2 receptor antagonist, ameliorates pathology in a rat Alzheimer’s modelPGD2 is a major brain prostaglandin and we report that timapiprant that is an antagonist for the PGD2 receptor DP2 reduces pathology and cognitive deficits in a transgenic rat model of Alzheimer’s.
Building in vitro models of the brain to understand the role of APOE in Alzheimer’s diseaseHuman induced pluripotent stem cell (hiPSC)–based models of the brain will be key to unraveling the role of APOE ɛ4 in the interconnected cellular changes underlying Alzheimer’s disease.
miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neuronsIn rat hippocampal neurons, miRNA-dependent regulation of the synaptic Prr7 protein is required for the homeostatic synaptic depression of excitatory synapses upstream of the CDK5-SPAR pathway.
Regulation of Liprin-α phase separation by CASK is disrupted by a mutation in its CaM kinase domainMutations in the human CASK gene cause a neurodevelopmental disorder; we show that CASK regulates condensate formation of Liprin-alpha 2 and that patient mutations in the CaM kinase domain interfere with Liprin binding and regulation of condensate formation.
Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar systemElectron tomography reveals four classes of cytopathic vesicles-II (CPV-II) stemming from the host Golgi apparatus after Venezuelan equine encephalitis virus infection.
A critique of the hypothesis that CA repeats are primary targets of neuronal MeCP2Using relevant neuronal datasets and in vitro experiments, we show that MeCP2 function depends on methylated CAC (mCAC) sites regardless of their occurrence in CA repeats or elsewhere in the genome.
ALS-linked loss of Cyclin-F function affects HSP90Analysis of ALS patient cell lines and cyclin-F overexpression and knockout cells identified HSP90AB1 as novel SCFcyclin-F substrate pointing to a loss-of-function mechanism for ALS CCNF mutations.
Ataxin-2, Twenty-four, and Dicer-2 are components of a noncanonical cytoplasmic polyadenylation complexIdentification of components of a noncanonical cytoplasmic polyadenylation machinery in Drosophila expands the diversity of RNA-binding proteins involved in poly(A) tail length control.
RSC and GRFs confer promoter directionality by restricting divergent noncoding transcriptionWe show that the chromatin remodelling complex RSC and general regulatory factors (GRFs) limit transcription in the noncoding divergent direction, thereby increasing the directionality of promoters.
