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LATS1 and LATS2 suppress breast cancer progression by maintaining cell identity and metabolic stateIn luminal B tumors LATS2 depletion results in metabolic rewiring whereas LATS1 depletion promotes the expression of basal-like features.
Phosphorylation of Arp2 is not essential for Arp2/3 complex activity in fission yeastThis work employed genomic substitutions blocking or mimicking phosphorylation at three proposed phosphorylation sites on Arp2 to demonstrate that they do not regulate Arp2/3 complex activity in fission yeast.
Topological in vitro loading of the budding yeast cohesin ring onto DNAThe biochemical reconstitution of topological DNA binding by budding yeast cohesin yields surprises and opens opportunities to exploit experimental approaches developed in this model organism.
Disruption of stromal hedgehog signaling initiates RNF5-mediated proteasomal degradation of PTEN and accelerates pancreatic tumor growthDisrupting paracrine Hedgehog signaling in pancreatic cancer stroma through genetic deletion of fibroblast Smoothened leads to proteasomal degradation of fibroblast PTEN and accelerates tumor growth.
The crystal structure of Staufen1 in complex with a physiological RNA sheds light on substrate selectivityCombination of in vitro and in vivo data show that RNA sequence influences Staufen target recognition and that protein–RNA base contacts are required for Staufen function in Drosophila.
Specificity for deubiquitination of monoubiquitinated FANCD2 is driven by the N-terminus of USP1Deubiquitination of FANCD2, FANCI, and PCNA by USP1 is essential for DNA repair signalling. Reconstitution of the system reveals that USP1-mediated specificity towards K561 of FANCD2 is directed by a unique sequence at USP1's N-terminus.
Loss of HDAC11 ameliorates clinical symptoms in a multiple sclerosis mouse modelIn an animal model for multiple sclerosis, the absence of HDAC11 reduces clinical severity, spinal cord demyelination, and immune cell infiltration, suggesting that HDAC11 is a promising target for MS treatment.
Secretory granule protein chromogranin B (CHGB) forms an anion channel in membranesThe CHGB subfamily of secretory granule proteins forms a new family of anion-selective channels by interacting with membranes via two amphipathic α-helices. The channel exhibits higher anion selectivity, larger conductance, higher DIDS-binding affinity, and higher Cl− sensitivity than other known anion channels.
Interaction modulation through arrays of clustered methyl-arginine protein modificationsExtensively modifiable arrays of clustered arginines in a large set of human proteins function as regulatory protein interaction platforms. Quantitative immunoprecipitation assays defined two distinct cumulative binding mechanisms operating across an array of 18 methyl-arginine motifs in SYNCRIP.
S-phase transcriptional buffering quantified on two different promotersTranscriptional buffering enforced during DNA replication shows that histone acetylation governs the homeostasis process and can also restrict promoters from reaching maximum transcriptional potential