More articles from Research Articles
Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency markerExpression of TET proteins in pluripotent cell types is visualised by epitope tagging of endogenous alleles.
Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forksFft3, a member of the SNF2 family of ATPase-dependent enzymes, plays a dual role at DNA replication barriers in fission yeast by promoting both DNA resection and restart of blocked DNA replication forks.
Sufficiency analysis of estrogen responsive enhancers using synthetic activatorsEnhancers bound by synthetic activators can recreate a transcriptional response to estrogen, drive different levels of gene expression, and work independently to regulate transcription.
HELZ directly interacts with CCR4–NOT and causes decay of bound mRNAsThe putative UPF1-like SF1 helicase HELZ directly interacts with the CCR4–NOT deadenylase complex to induce translational repression and 5′-to-3′ decay of bound mRNAs.
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modificationsThe glycine cleavage system (GCS) is highly activated to promote stem cell pluripotency. The GCS catabolizes glycine to prevent methylglyoxal accumulation and to fuel H3K4me3 modification, promoting the expression of pluripotency genes.
Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachineThe article shows successful engineering of the syringe-like injection apparatus of Photorhabdus luminescens as standalone nanomachine for delivery of protein toxins and enzymes from different species into mammalian cells.
Mechanistic insights into the protective roles of polyphosphate against amyloid cytotoxicityThis study provides novel insights into the mechanisms by which presence of polyP alters the formation, structural properties, and cytotoxic effects of α-synuclein fibers.
The huntingtin inclusion is a dynamic phase-separated compartmentWhen expressed in yeast, mutant Htt-GFP forms an inclusion that is mobile, gel-like, and releases material; evidence suggests that it grows through collision and coalescence with small aggregates.
Pleiotropic requirements for human TDP-43 in the regulation of cell and organelle homeostasisComprehensive analysis of human TDP-43 knockout cells revealed that TDP-43 regulates hundreds of transcripts and is critical for maintaining the homeostasis of multiple subcellular organelles.
Structural and functional characterization of the mitochondrial complex IV assembly factor Coa6The structures of the mitochondrial complex IV assembly factor Coa6 and a pathogenic mutant variant (W59CCoa6) are reported, providing molecular mechanisms for its mode of action and the loss-of-function mutation.