More articles from Research Articles
multicrispr: gRNA design for prime editing and parallel targeting of thousands of targetsA bioinformatics R package for a fast and generic guideRNA design, optimized for large-scale perturbation assays.
MicroRNA regulation of murine trophoblast stem cell self-renewal and differentiationPost-transcriptional regulation of CDX2 and cell cycle genes by miR-290 and miR-322 clusters along with transactivation of miR-290 cluster and cyclin D1 by CDX2 equipoise trophoblast stem cell self-renewal and differentiation.
Multiplexed chemogenetics in astrocytes and motoneurons restore blood–spinal cord barrier in ALSChemogenetic motoneuron excitation and astrocyte GPCR-Gi signaling restore blood–spinal cord barrier, disrupted in four ALS mouse models, revealing its role in disease progression but not initiation.
An atlas of human proximal epididymis reveals cell-specific functions and distinct roles for CFTRA coordinated process of maturation in the lumen of the epididymis is required for sperm to fertilize an egg. A single cell atlas of the human proximal epididymis reveals key cell-specific functions, regional specialization, and two roles for CFTR.
A dichotomy of gene regulatory associations during the activated B-cell to plasmablast transitionAs human B-cells differentiate, IRF4 switches occupancy and acquires CTCF colocalisation and IRF4 regulatory output diverts from cell activation to plasma cell state genes in conjunction with XBP1, whereas BLIMP1 represses activation.
Plasma membrane phosphatidylinositol (4,5)-bisphosphate promotes Weibel–Palade body exocytosisPhosphatidylinositol (4,5)-bisphosphate transiently accumulates at sites of Weibel–Palade body–plasma membrane fusion and promotes agonist-evoked exocytosis of endothelial von-Willebrand factor.
Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assemblyThe AAA+ protein Pch2 forms a biochemical complex with Orc1/ORC to suppress DNA break formation in the meiotic G2/prophase.
PGE2 accounts for bidirectional changes in alveolar macrophage self-renewal with aging and smokingDysregulation of self-renewal contributes to alterations in alveolar macrophages (AMs). In this study, Penke et al identify PGE2 as a suppressor of AM proliferation, whose divergent bioavailability in aged and cigarette smoke-exposed mice could explain opposite effects on AM numbers.
The RECQL helicase prevents replication fork collapse during replication stressThis work shows that RECQL is an essential player in the protection of stalled replication forks and possibly the restart of broken replication forks, thereby representing a target for cancer therapy.
DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesisIn intestinal epithelial cells (IECs), DDX5 promotes the expression of immune response genes and oncogenes posttranscriptionally and is a novel therapeutic target for treating colitis and intestinal cancers.