More articles from Research Articles
In vivo CRISPR/Cas9 knockout screen: TCEAL1 silencing enhances docetaxel efficacy in prostate cancerA whole genome in vivo CRISPR/Cas9 screen identifies TCEAL1 as a potential target to sensitise prostate cancer cells to docetaxel to improve the efficacy of chemotherapy.
Neurturin regulates the lung-resident macrophage inflammatory response to viral infectionThe neurotrophic factor RET is induced in lung macrophages by viral triggers and activation via its ligand neurturin regulates matrix proteins and cytokines that shape the inflammatory response.
Gene selection for optimal prediction of cell position in tissues from single-cell transcriptomics dataWe describe and provide an array of diverse methods to predict cellular positions in tissue from RNA-seq data selecting mapping genes according to their spatial/statistical properties and their effect on improving the cell positioning.
Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeastThe canonical LINC complex is composed of two different transmembrane proteins; this work reveals the heterotrimeric composition of the telomere-associated LINC complex in budding yeast.
Fungal mycelia and bacterial thiamine establish a mutualistic growth mechanismBacterial cells travel along fungal highway and pay thiamine as a toll to the fungus. Simultaneous spatial and metabolic interactions in communicating bacterial and fungal species establish a mutualism that facilitates them to obtain an environmental niche and nutrient.
Predicting gene regulatory networks from cell atlasesIntegrated single-cell gene regulatory network from three mouse cell atlases captures global and cell type–specific regulatory modules and crosstalk, important for cellular identity.
Human G-MDSCs are neutrophils at distinct maturation stages promoting tumor growth in breast cancerThis study shows that immunosuppressive primary breast cancer patient–derived G-MDSCs (PMN-MDSCs) are neutrophils at a range of maturations stages, and provides in vivo evidence for that human G-MDSCs also promote tumor growth and myeloid immune cell exclusion.
Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-FCrystal and single-particle cryo-EM structures reveal how the tetraspanin CD9 interacts with its prototypical partner EWI-F and provide a new concatenation model for the assembly of tetraspanin-enriched microdomains.
Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4The work determines the relative and absolute stoichiometry of a 5-chain protein complex involved in histone chaperoning and acetylation. Using sedimentation velocity and light scattering, it uncovers a dynamic equilibrium of complex self-association.
multicrispr: gRNA design for prime editing and parallel targeting of thousands of targetsA bioinformatics R package for a fast and generic guideRNA design, optimized for large-scale perturbation assays.