More articles from Research Articles
Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m5C at GCU motifsPublished predictions of 5-methylcytosine at GCU sites inferred from direct RNA sequencing and the Tombo Alternative Model should be reconsidered unless further validated with orthogonal methods.
Analysis of the indispensable RAD51 cofactor BRCA2 in Naganishia liquefaciens, a Basidiomycota yeastIdentification of the BRCA2 homolog in the Basidiomycota yeast Naganishia liquefaciens reveals the general attributes of fungal BRCA2 and its essential role in Rad51-mediated homologous recombination.
PARP-1 is a transcriptional rheostat of metabolic and bivalent genes during developmentPARP-1 fine-tunes gene expression to control energy balance and development by selectively repressing and activating critical genes for developmental progression.
2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibitionThis study investigates the genome-wide effects of 2-HG accumulation on chromatin and gene expression and identifies Rph1-dependent and H3K36me3-associated gene repression at silenced loci in yeast.
Endothelial cells adopt a pro-reparative immune responsive signature during cardiac injuryscRNA-seq uncovers an endothelial cell (EC) subpopulation with immune responsive signatures in cardiac injury. Profiling of human heart failure samples reveals EC-specific immune responsive signatures in adult and pediatric heart failure. ECs can adopt immune responsive signatures during cardiac stress.
A dual-targeted drug inhibits cardiac ryanodine receptor Ca2+ leak but activates SERCA2a Ca2+ uptakeOur study identified a first multi-targeted compound (GM1869) inhibiting RyR2-mediated Ca2+ leak together with activating SERCA2a function that may represent a critical breakthrough for treatment of diastolic Ca2+ leak in heart failure.
RNA helicase EIF4A1-mediated translation is essential for the GC responseConditional mouse knock-out models show that EIF4A1, but not its cofactors EIF4B or EIF4H, is essential in B cells for their development, the GC response, and increased translation after activation.
Decline of DNA damage response along with myogenic differentiationIn postmitotic myotubes, DNA repair processes such as Base Excision Repair, and Non-Homologous End Joining are weakened compared with the proliferative precursors myoblasts.
Megakaryocytes possess a STING pathway that is transferred to platelets to potentiate activationThis work reveals that megakaryocytes transfer cGAS and STING proteins to platelets during thrombopoiesis and that cGAS stimulation results in the activation and aggregation of platelets, suggesting a new role of cGAS in thromboinflammation.
Enhancer-driven 3D chromatin domain folding modulates transcription in human mammary tumor cellsThe study shows that estrogen activates transcription within pre-established chromosomal 3D architectures, strengthening enhancer interactions through local accumulation of the estrogen receptor α.