More articles from Methods
Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epitheliaThis work describes an optimized assay to determine CFTR drug responses in people with cystic fibrosis, using nasal-airway organoids that are generated from 2D differentiated epithelial monolayers.
Workflow for high-dimensional flow cytometry analysis of T cells from tumor metastasesWe describe an improved method for the high-dimensional flow cytometry analysis of conventional and rare unconventional T cells infiltrating tumor liver metastases.
Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencingThis article proposed the correctable decoding sequencing technology with conservative theoretical error rate of 0.0009%, and evaluated its robustness by simulation. This technology can provide a powerful new protocol for NGS platforms, enabling accurate identification of rare mutations in medicine.
Tracking connectivity maps in human stem cell–derived neuronal networks by holographic optogeneticsHolographic optogenetic stimulation of human iPSC–derived neuronal networks was exploited to map precise functional connectivity motifs and their long-term dynamics during network development.
A FIJI macro for quantifying pattern in extracellular matrixThis work provides a quantitative tool for the analysis of fibrillar patterns in biology. We demonstrates its utility on in vitro generated extracellular matrices, mouse tissues, and patient samples. The tool is already publicly available to the research community.
Quantification of cristae architecture reveals time-dependent characteristics of individual mitochondriaA novel, machine-learning approach to quantify cristae density and architecture in live-cell super-resolution imaging characterizes the ultrastructural parameters of individual mitochondria over time.
Robust production of uniform human cerebral organoids from pluripotent stem cellsBy leveraging advances in 3D stem cell culture techniques, the authors describe, characterize and validate a novel platform to efficiently generate morphologically consistent human forebrain cerebral organoids.
Simultaneous multiple allelic replacement in the malaria parasite enables dissection of PKG functionThis work combines the use of an inducible Cre recombinase and different lox sites to simultaneously create multiple allelic replacements. We show that an essential malarial cGMP-dependent protein kinase functions exclusively during the release of the parasite from its host cell.
MagnEdit—interacting factors that recruit DNA-editing enzymes to single base targetsThis study reports a new, non-covalent strategy—called MagnEdit—that attracts the DNA cytosine deaminase APOBEC3B to a Cas9-directed site for C-to-T editing.
In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joiningThis study provides a new method that enables the labelling of knockout cells with fluorescent protein through microhomology-mediated end joining (MMEJ)–based knock-in.