More articles from Methods
Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generationThis article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
Spatial metabolomics for symbiotic marine invertebratesMass spectrometry imaging revealed in situ spatial metabolite distribution patterns at 50 μm resolution for sea anemones and scleractinian corals and their microalgal endosymbionts.
Evaluation of genetic demultiplexing of single-cell sequencing data from model speciesThe authors demonstrate that scRNA-seq sample pooling followed by genetics-based separation of individuals is an effective means to identify individual samples in a variety of commonly studied species.
BiFCo: visualizing cohesin assembly/disassembly cycle in living cellsA novel strategy developed in fission yeast to visualise only-assembled cohesin complex loading/unloading cycles in eukaryotic chromosomes in living cells.
Rapid and precise genotyping of transgene zygosity in mice using an allele-specific methodA novel, precise, rapid, and generalizable method to determine transgene zygosity in mice by allele-specific restriction enzyme digestion of PCR products from transgenes or homologous endogenous genes.
A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenatesThis novel method can be used in clinical samples to determine CV function in diseases, with the added benefit of being able to use frozen samples in a high-throughput manner and to explore ATP hydrolysis as a drug target for disease treatment.
A protocol for single nucleus RNA-seq from frozen skeletal muscleThe authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples.
Efficient knock-in method enabling lineage tracing in zebrafishThis study introduces a knock-in method in zebrafish that is cloning-free, preserves the endogenous gene, achieves high germline transmission, and useful for both cell labelling and lineage tracing.
New algorithms for accurate and efficient de novo genome assembly from long DNA sequencing readsInnovative algorithmic approaches were used to perform assembly of complex genomes across the tree of life, from long DNA sequencing data.
Plasma proteome dynamics of COVID-19 severity learnt by a graph convolutional network of multi-scale topologyWe used a graph convolutional network to model the plasma proteome of 384 COVID-19 patients. The model incorporates clinical data and is able to predict severity. We identified the proteins that shifted most in expression depending on severity.