More articles from Methods
Cell-Int: a cell–cell interaction assay to identify native membrane protein interactionsA cell interaction assay to study membrane protein interactions in quasi-physiological settings, with multiple applications, from deciphering interaction networks to assessing therapeutic molecules.
Identification of single-cell blasts in pediatric acute myeloid leukemia using an autoencoderThe authors present a novel automated approach for the identification of blasts and their developmental stage in a longitudinal pediatric acute myeloid leukemia (AML) cohort. KMT2A-rearranged AMLs show unstable immunophenotype, with most patients presenting a more differentiated phenotype at relapse.
A scalable approach to topic modelling in single-cell data by approximate pseudobulk projectionRandom projection of pseudobulk gene expression data improves the scalability and accuracy of single-cell topic modelling.
pyRBDome: a comprehensive computational platform for enhancing RNA-binding proteome dataThis study explores enhancing protein–RNA interaction prediction using machine learning.
Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1This study reports the first nanobodies against the mitochondrial fission protein Drp1 for application in proteomics, high-resolution microscopy and live cell imaging.
Combining sophisticated fast FLIM, confocal microscopy, and STED nanoscopy for live-cell imaging of tunneling nanotubesHere, we proposed an innovative advanced light imaging to reveal the dynamics of tunneling nanotubes (TNTs) that are now considered as a long- and short-distance route for cell-to-cell communication.
Generation of marmoset primordial germ cell–like cells under chemically defined conditionsMarmoset primordial germ cells (PGCs) express SOX17, AP2Ɣ, and BLIMP1 in vivo, and under specific feeder-free conditions, marmoset iPSCs can be induced into PGC-like cells in vitro.
RedRibbon: A new rank–rank hypergeometric overlap for gene and transcript expression signaturesRedRibbon is a comparative analysis tool of differential omics analyses to reveal overlapping features between two differential studies, with high performance, accuracy, and simplicity in use.
A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cellsThis work describes a high-throughput cellular assay specifically designed to study the understudied inhibitory signalling pathway in T cells.
Mitochondria isolated from lipid droplets of white adipose tissue reveal functional differences based on lipid droplet sizeIn the current study, we show an optimized protocol using proteolytic treatment that enables the isolation of peridroplet mitochondria (PDM) from white adipose tissue (WAT), revealing a unique function of WAT PDM that depends on the heterogeneity of lipid droplet size.