The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro

Xiangyi S Wang; Jenny Jiou; Anthony Cerra; Simon A Cobbold; Marco Jochem; Ka Hin Toby Mak; Leo Corcilius; John Silke; Richard J Payne; Ethan D Goddard-Borger; David Komander; Bernhard C Lechtenberg

doi:10.26508/lsa.202503243

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The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro

View ORCID ProfileXiangyi S Wang, View ORCID ProfileJenny Jiou, View ORCID ProfileAnthony Cerra, Simon A Cobbold, Marco Jochem, Ka Hin Toby Mak, View ORCID ProfileLeo Corcilius, View ORCID ProfileJohn Silke, Richard J Payne, Ethan D Goddard-Borger, View ORCID ProfileDavid Komander, View ORCID ProfileBernhard C Lechtenberg Correspondence email

Xiangyi S Wang

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Conceptualization, Resources, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing—original draft, Writing—review and editing

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ORCID record for Xiangyi S Wang

Jenny Jiou

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Validation, Investigation, Visualization, Methodology, Writing—review and editing

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ORCID record for Jenny Jiou

Anthony Cerra

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Data curation, Validation, Investigation, Visualization, Methodology, Writing—review and editing

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ORCID record for Anthony Cerra

Simon A Cobbold

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing—review and editing

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Marco Jochem

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing—review and editing

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Ka Hin Toby Mak

3School of Chemistry, The University of Sydney, Sydney, Australia

4Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, Sydney, Australia

Roles: Resources, Investigation, Methodology, Writing—review and editing

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Leo Corcilius

3School of Chemistry, The University of Sydney, Sydney, Australia

4Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, Sydney, Australia

Roles: Resources, Funding acquisition, Investigation, Methodology, Writing—review and editing

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ORCID record for Leo Corcilius

John Silke

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

5Inflammation Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Methodology, Writing—review and editing

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ORCID record for John Silke

Richard J Payne

3School of Chemistry, The University of Sydney, Sydney, Australia

4Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, Sydney, Australia

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Methodology, Writing—review and editing

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Ethan D Goddard-Borger

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

6ACRF Chemical Biology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

Roles: Conceptualization, Resources, Funding acquisition, Visualization, Methodology, Writing—review and editing

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David Komander

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Methodology, Writing—review and editing

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ORCID record for David Komander

Bernhard C Lechtenberg

1Ubiquitin Signalling Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

2Department of Medical Biology, The University of Melbourne, Parkville, Australia

Roles: Conceptualization, Resources, Data curation, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Project administration, Writing—original draft, Writing—review and editing

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ORCID record for Bernhard C Lechtenberg
For correspondence: lechtenberg.b@wehi.edu.au

Published 1 April 2025. DOI: 10.26508/lsa.202503243

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Ubiquitination of non-protein substrates by HOIL-1

Xiangyi S Wang, Jenny Jiou, Anthony Cerra, Simon A Cobbold, Marco Jochem, Ka Hin Toby Mak, Leo Corcilius, John Silke, Richard J Payne, Ethan D Goddard-Borger, David Komander, Bernhard C Lechtenberg

Life Science Alliance Apr 2025, 8 (6) e202503243; DOI: 10.26508/lsa.202503243

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Ubiquitination of non-protein substrates by HOIL-1

Xiangyi S Wang, Jenny Jiou, Anthony Cerra, Simon A Cobbold, Marco Jochem, Ka Hin Toby Mak, Leo Corcilius, John Silke, Richard J Payne, Ethan D Goddard-Borger, David Komander, Bernhard C Lechtenberg

Life Science Alliance Apr 2025, 8 (6) e202503243; DOI: 10.26508/lsa.202503243

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