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Research Articles
TPX2 lactylation is required for the cell cycle regulation and hepatocellular carcinoma progressionThis study identifies TPX2 lactylation as a critical regulator of hepatocellular carcinoma progression, linking metabolic reprogramming to cell cycle dysregulation.
Divergent Plasmodium kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesisThis study reveals how Plasmodium kinases regulate MTOC, axoneme, and kinetochore organisation in male gametogenesis, providing key insights into potential targets for malaria transmission control.
Antibodies targeting ADAM17 reverse neurite outgrowth inhibition by myelin-associated inhibitorsInhibitory anti-ADAM17 monoclonal antibodies (mAbs) abrogate the cleavage of p75 in a neuroblastoma-glioma cell line.
Rapid ER remodeling induced by a peptide–lipid complex in dying tumor cellsAlpha1-oleate induces novel ER remodeling, causing peripheral ER loss, vesiculation, and nuclear entry, expanding the nuclear ER with its content of dying cell debris.
Molecular mechanism of bacteriophage contraction structure of an S-layer–penetrating bacteriophageThis study describes the high-resolution cryoEM structure of a contractile bacteriophage, displaying reduced contraction and lack of enzymatic domains for penetration.
Rheological transition driven by matrix makes cancer spheroids resilient under confinementThe formation of lumen and increased E-cadherin levels driven by a basement membrane–like matrix of ovarian cancer spheroids impart them with elastic mechanics and resilience under confinement flow.
The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitroThe RBR E3 ligase HOIL-1 ubiquitinates Ser/Thr residues and various disaccharides in vitro and can be used to generate ubiquitinated sugars as tools to study non-proteinaceous ubiquitination.
The MFN2 Q367H variant reveals a novel pathomechanism connected to mtDNA-mediated inflammationThe present study describes a novel pathogenic variant in MFN2 from a patient with late-onset distal myopathy and links pathogenic perturbation of MFN2 function to mitochondrial DNA release and inflammation.
Plasmacytoid dendritic cell sensing of hepatitis E virus is shaped by both viral and host factorsClose contacts between HEV-infected cells and plasmacytoid dendritic cells trigger interferon production in a TLR7- and ORF2-dependent manner, leading to the inhibition of viral replication.
Shared requirement for MYC upstream super-enhancer region in tissue regeneration and cancerA region located upstream of the MYC oncogene that carries more risk for inherited cancer than any other human genomic region is required for intestinal regeneration.
Adenosine-generating CD39+ plasmablasts predispose to successful infliximab therapy in pediatric IBDIFX responders showed increased CD39 expression on plasmablasts and higher levels of CD39/CD73 co-expression on naïve and memory B cells, along with enhanced ATP degradation and adenosine production before treatment. Response was associated with increased IL10-producing plasmablasts and α4β7hi plasmablasts.
DAF-18/PTEN protects LIN-35/Rb from CLP-1/CAPN-mediated cleavage to promote starvation resistanceDAF-18/PTEN acts independently of insulin/IGF signaling to promote starvation resistance via LIN-35/Rb and the DREAM complex in C. elegans L1 arrest.
Resources
Cytokine storm and vascular leakage in severe dengue: insights from single-cell RNA profilingFixed single-cell RNA profiling identified IL-17A/F-producing γδ T cells, ILC3s, and Th17 cells, cooperative roles of M1- and M2-like macrophages, and dual roles of neutrophils during severe dengue.
Methods
A novel human organoid model system reveals requirement of TCF4 for oligodendroglial differentiationIn this study, we developed a cell system to study TCF4 in human oligodendrocyte differentiation, showed that TCF4 regulates human oligodendroglial differentiation in a dose-dependent manner, and established a system to dissect TCF4 function in a human tissue–like context.
Efficient identification of de novo mutations in family trios: a consensus-based informatic approachResearchers developed a highly precise and accurate approach for identifying de novo genetic variants in probands from trio genome sequencing, making it suitable for automated large-scale analyses.
Predicting cell cycle stage from 3D single-cell nuclear-stained imagesCellCycleNet simplifies cell cycle staging from microscopy images using only a nuclear stain (DAPI). Trained on Fucci2a reporter data, our 3D deep learning model achieves high classification accuracy (AUROC = 0.95), providing a cost-effective, intervention-free tool for cell cycle profiling.
Optogenetic control of pheromone gradients and mating behavior in budding yeastThe authors combined theory and experiment to develop light-induced yeast pheromone gradients that spatially control growth, mating and morphology in hydrogel-embedded cells.
