Table of Contents
Research Articles
Mitochondrial signatures shape phenotype switching and apoptosis in response to PLK1 inhibitorsSpecific mitochondrial proteins are associated with resistance to treatments targeting polo-like kinase 1 activity or expression in melanoma. Resistant cells are less apoptotic and trigger a transcriptional program of dedifferentiation and a pro-inflammatory phenotype.
Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cellsA screen for NOTCH1 localization and signaling in breast epithelial cells identifies 3 new genes that regulate receptor trafficking and abundance.
Defective glycosylation and ELFN1 binding of mGluR6 congenital stationary night blindness mutantsThis study demonstrates that several night blindness mutations in a retinal postsynaptic glutamate receptor lead to altered post-translational modifications and failure to bind to a presynaptic partner.
Molecular analysis of acute pyelonephritis—excessive innate and attenuated adaptive immunityCharacteristics of a cytokine storm, with hyper-activated innate immunity and attenuated adaptive immunity in infants with acute pyelonephritis, defined by gene expression, urine protein, and exome sequence analysis.
Genetic modulation of RNA splicing rescues BRCA2 function in mutant cellsThis study provides proof of principle for the therapeutic potential of splicing modulation as a strategy to rescue BRCA2 function in mutant cells.
NSD3 protein methylation and stabilization transforms human ES cells into variant stateOur study highlights the role of epigenetic machinery in transformation of normal pluripotent stem cells to variant pluripotent state. We demonstrate the importance of non-histone protein methylation, which underlie the EMT and abnormal differentiation behaviour of variant hESCs.
Mechanical compressive forces increase PI3K output signaling in breast and pancreatic cancer cellsThis article provides a proof-of-concept showing that the therapeutic impact of targeted therapies on solid cancer cells is dependent on compressive mechanical context.
lncRNA CARINH regulates expression and function of innate immune transcription factor IRF1 in macrophagesThe antisense lncRNA CARINH acts as an additional regulatory layer of antiviral immunity through coordinated regulation of the transcription factor IRF1 and downstream interferon-stimulated genes.
COA5 has an essential role in the early stage of mitochondrial complex IV assemblyThe mitochondrial protein COA5 was previously reported as a complex IV assembly factor. Studying a further case of COA5-related mitochondrial disease, the authors delineate an essential role of this protein in the early stage of complex IV assembly.
Resources
Systematic assessment of structural variant annotation tools for genomic interpretationThis study benchmarks eight structural variant prioritization tools, highlighting their comparable effectiveness in predicting pathogenicity and providing insights for improved genomic research.
Methods
DICE: fast and accurate distance-based reconstruction of single-cell copy number phylogeniesThis work presents two new computational methods for reconstructing tumor cell lineage trees from single-cell copy number data.
FrozONE: quick cell nucleus enrichment for comprehensive proteomics analysis of frozen tissuesThe authors establish and benchmark a nuclear proteomics workflow that is faster, simpler, and more scalable than classical protocols, while being as efficient, specific, and reproducible.
Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantificationConcatemer-assisted stoichiometry analysis (CASA) addresses a common challenge in cell biology: absolute quantification of protein subunits in native protein complexes.
Reviews
Divergent destinies: insights into the molecular mechanisms underlying EPI and PE fate determinationThis review explores the molecular mechanisms governing epiblast and primitive endoderm fate determination during murine pre-implantation development, dissecting this lineage specification process into four stages to provide a comprehensive framework for understanding their establishment and maintenance.
