Table of Contents
Research Articles
Noncanonical altPIDD1 protein: unveiling the true major translational output of the PIDD1 geneThis research uncovers PIDD1 as a dual-coding gene, revealing a previously unknown protein, altPIDD1, as the primary product with higher expression and cytoskeletal interactions.
Nef mediates neuroimmune response, myelin impairment, and neuronal injury in EcoHIV-infected miceAntiretroviral therapy (ART) has improved HIV-associated neurocognitive disorder (HAND) outcomes, but nearly half of individuals remain affected. This study reveals how the HIV protein Nef drives neuroinflammation and neuronal damage, highlighting potential therapeutic targets for HAND-related neuropathology.
KAT6B is required for histone 3 lysine 9 acetylation and SOX gene expression in the developing brainHeterozygous mutations in the MYST family histone lysine acetyltransferase, KAT6B, cause intellectual disability disorders. This study identifies the histone and gene targets of KAT6B which include SOX2, a master regulator of neural stem and progenitor cells.
Vinculin–Arp2/3 interaction inhibits branched actin assembly to control migration and proliferationVinculin, a mechanotransducer protein of focal adhesion and adherens junction, inhibits branched actin assembly through Arp2/3 interactions to control membrane protrusion, cell migration, cell–cell junctions, and cell cycle progression.
Identification of phosphatases that dephosphorylate the co-chaperone BAG3PP1 catalyses the dephosphorylation at BAG3-pS136, inhibiting its association with 14-3-3 proteins. PP5 targets a BAG3 phosphorylation-site cluster, including pT285, regulating the interaction of BAG3 with HspB8 as well as BAG3-mediated protein degradation.
Brain and behavioural anomalies caused by Tbx1 haploinsufficiency are corrected by vitamin B12The study shows that mice that are a model of 22q11.2 deletion syndrome have abnormal brain metabolism, and it identifies potential biomarkers of metabolic brain disease in 22q11.2DS patients.
Fatty acid synthase inhibition alleviates lung fibrosis via β-catenin signal in fibroblastsIdiopathic pulmonary fibrosis (IPF) is a fatal disease marked by activated fibroblasts. This study shows fatty acid synthase (FASN) inhibition induces quiescent fibroblasts, reduces β-catenin, and alleviates pulmonary fibrosis, suggesting FASN as a therapeutic target.
Vascular dysfunction is at the onset of oxaliplatin-induced peripheral neuropathy symptoms in miceThis study identifies blood-nerve barrier and vascular contraction dysfunction in nerve blood vessels as contributors to oxaliplatin-induced peripheral neuropathy (OIPN), and demonstrates that vasodilators can alleviate neuropathic symptoms and nerve hypoxia, highlighting the importance of vascular health in OIPN management.
Membrane transporters modulating the toxicity of arsenic, cadmium, and mercury in human cellsThis study employs CRISPR/Cas9 loss-of-function screens to simultaneously investigate the contribution of the entire transportome to regulating the toxicity of arsenic, cadmium, and mercury in human cell lines.
Unremodeled GPI-anchored proteins at the plasma membrane trigger aberrant endocytosisA unique plasma membrane stressor induces vacuolar degradation of abnormally endocytosed proteins.
SPHK1/S1PR1/PPAR-α axis restores TJs between uroepithelium providing new ideas for IC/BPS treatmentThis study provides a novel therapeutic idea based on the poor limited efficacy of IC/BPS treatment options. Using urinary metabolite and tight junctions as therapeutic targets is revelatory.
Morphoregulatory ADD3 underlies glioblastoma growth and formation of tumor–tumor connectionsMorphoregulatory ADD3 controls glioblastoma stem cell (GSC) connectivity, proliferation, and chemoresistance, showing that GSC morphology is a new layer of tumor heterogeneity.
ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose toleranceciBAR1-KO mice exhibit ciliopathy phenotypes such as embryonic lethality and exocrine and endocrine pancreatic defects, indicating that ciBAR1 plays a critical role in ciliogenesis in vivo.
Hypoxia favors tumor growth in colorectal cancer in an integrin αDβ1/hemoglobin δ-dependent mannerColorectal cancers co-express the αDβ1 integrin and hemoglobin δ, and down-regulation of either protein in hypoxia inhibited oxygen uptake and cell proliferation.
N-6-methyladenosine (m6A) promotes the nuclear retention of mRNAs with intact 5′ splice site motifsmRNAs with intact 5′SS motifs must be m6A-modified to be nuclear retained by a conserved pathway that includes YTHDC1/YTHDC2, ZFC3H1 (a PAXT complex component), and U1-70K (a component of the U1 snRNP).
RuvBL1/2 reduce toxic dipeptide repeat protein burden in multiple models of C9orf72-ALS/FTDEnhancing RuvBL1, but particularly RuvBL2 expression, reduces toxic dipeptide repeat proteins in vitro and in vivo models of C9orf72-linked ALS/FTD, suggesting that modulating RuvBL1/2 levels could be a promising therapeutic approach for C9ALS/FTD.
Depleting chemoresponsive mitochondrial fission mediator DRP1 does not mitigate sarcoma resistanceThe mitochondrial fission mediator DRP1 levels and activation are modulated upon chemotherapy exposure, yet depleting DRP1 does not restore chemosensitivity in the most common pediatric sarcomas.
Methods
Computing hematopoiesis plasticity in response to genetic mutations and environmental stimulationsAn integrated and single-cell-omics dataset–based computing pipeline scPlasticity is introduced to quantify cell plasticity and prioritize master regulators in normal and stress hematopoiesis.
Endo-bind-n-seq: identifying RNA motifs of RNA binding proteins isolated from endogenous sourcesRNA endo-bind-n-seq allows for the identification of RNA elements that are bound by RBPs, which are isolated from their physiological environments using immunoprecipitation and RNA selection.
Corrections
- Correction: Microglia are essential for tissue contraction in wound closure after brain injury in zebrafish larvae
Although in humans, the brain fails to heal after an injury, young zebrafish are able to restore tissue structural integrity in less than 24 h, thanks to the mechanical action of microglia.
