Reverse-engineering the anti-MUC1 antibody 139H2 by mass spectrometry–based de novo sequencing

Weiwei Peng; Koen CAP Giesbers; Marta Šiborová; J Wouter Beugelink; Matti F Pronker; Douwe Schulte; John Hilkens; Bert JC Janssen; Karin Strijbis; Joost Snijder

doi:10.26508/lsa.202302366

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Reverse-engineering the anti-MUC1 antibody 139H2 by mass spectrometry–based de novo sequencing

Weiwei Peng, Koen CAP Giesbers, Marta Šiborová, View ORCID ProfileJ Wouter Beugelink, View ORCID ProfileMatti F Pronker, View ORCID ProfileDouwe Schulte, John Hilkens, View ORCID ProfileBert JC Janssen, View ORCID ProfileKarin Strijbis Correspondence email, View ORCID ProfileJoost Snijder Correspondence email

Weiwei Peng

1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

Roles: Formal analysis, Investigation, Visualization, Methodology, Writing—original draft, Writing—review and editing

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Koen CAP Giesbers

2Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands

Roles: Formal analysis, Investigation, Visualization, Methodology, Writing—review and editing

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Marta Šiborová

1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

Roles: Formal analysis, Investigation, Visualization, Methodology, Writing—review and editing

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J Wouter Beugelink

3Structural Biochemistry, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Utrecht, Netherlands

Roles: Formal analysis, Investigation, Visualization, Methodology, Writing—review and editing

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ORCID record for J Wouter Beugelink

Matti F Pronker

1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

Roles: Formal analysis, Investigation, Visualization, Methodology, Writing—review and editing

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ORCID record for Matti F Pronker

Douwe Schulte

1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

Roles: Data curation, Software, Writing—review and editing

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ORCID record for Douwe Schulte

John Hilkens

4Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, Netherlands

Roles: Resources

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Bert JC Janssen

3Structural Biochemistry, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, Utrecht, Netherlands

Roles: Resources, Supervision, Investigation, Writing—review and editing

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ORCID record for Bert JC Janssen

Karin Strijbis

2Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Investigation, Writing—review and editing

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ORCID record for Karin Strijbis
For correspondence: k.strijbis@uu.nl

Joost Snijder

1Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Investigation, Writing—original draft, Writing—review and editing

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ORCID record for Joost Snijder
For correspondence: j.snijder@uu.nl

Published 20 March 2024. DOI: 10.26508/lsa.202302366

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Sequencing an anti-MUC1 mAb by mass spec

Weiwei Peng, Koen CAP Giesbers, Marta Šiborová, J Wouter Beugelink, Matti F Pronker, Douwe Schulte, John Hilkens, Bert JC Janssen, Karin Strijbis, Joost Snijder

Life Science Alliance Mar 2024, 7 (6) e202302366; DOI: 10.26508/lsa.202302366

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Sequencing an anti-MUC1 mAb by mass spec

Weiwei Peng, Koen CAP Giesbers, Marta Šiborová, J Wouter Beugelink, Matti F Pronker, Douwe Schulte, John Hilkens, Bert JC Janssen, Karin Strijbis, Joost Snijder

Life Science Alliance Mar 2024, 7 (6) e202302366; DOI: 10.26508/lsa.202302366

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