Table of Contents
Reviews
CXCR4: from B-cell development to B cell–mediated diseasesThis review provides an overview of the role of the C-X-C chemokine receptor type 4 (CXCR4) in B-cell development and in B cell–mediated disorders.
Research Articles
Genetic ablation of Immt induces a lethal disruption of the MICOS complexA new conditional mouse model deleting Immt/MIC60 reveals the necessity of this protein in vivo and provides an important resource for future research into the mitochondrial MICOS complex.
CRL4DCAF1 ubiquitin ligase regulates PLK4 protein levels to prevent premature centriole duplicationThis study identifies the E3 ubiquitin ligase, CRL4DCAF1, as a new regulator of polo-like kinase 4, PLK4, protein levels in the G2 phase of the cell cycle to prevent premature duplication of centrioles.
Aberrant DNA methylation distorts developmental trajectories in atypical teratoid/rhabdoid tumorsThis study describes how aberrant DNA methylation–driven epigenetic regulation affects DNA binding of key transcriptions and maintains the malignant, low differentiation cell state in AT/RTs.
SIRT4 as a novel interactor and candidate suppressor of C-RAF kinase in MAPK signalingThis study proposed a novel extra-mitochondrial role of the tumor suppressor SIRT4 in negatively regulating the MAPK pathway by interacting with C-RAF kinase.
Immunogenicity and effectiveness of an mRNA therapeutic vaccine for HPV-related malignanciesmHTV-02 functions as a candidate effective therapeutic mRNA-LNP vaccine via intramuscular or intratumoral administration routes for treating malignancies caused by HPV16 or HPV18 infections.
The Rtf1/Prf1-dependent histone modification axis counteracts multi-drug resistance in fission yeastMutations in a conserved histone modification axis confer a unique drug-tolerant phenotype in fission yeast, and suggest a mode of gene regulation shared with the Rpb1 C-terminal domain.
Novel chemotype NLRP3 inhibitors that target the CRID3-binding pocket with high potencyThe NLRP3 inflammasome drives various diseases. Here, we describe novel potent and selective NLRP3-targeted inhibitors to bolster pharmacological studies and development of NLRP3-targeted therapies.
Type I interferon regulates interleukin-1beta and IL-18 production and secretion in human macrophagesInterferon type I modulates the gene expression of inflammasome-related genes and reduces cytokine release induced by NLRP3 inflammasome activation in human macrophages in vitro.
Linc00673-V3 positively regulates autophagy by promoting Smad3-mediated LC3B transcription in NSCLCLinc00673-V3 isoform protects Smad3 from STUB1-mediated degradation and accumulated Smad3 facilitates LC3B transcription which ultimately promotes autophagy and chemoresistance in NSCLC.
pADP-ribosylation regulates the cytoplasmic localization, cleavage, and pro-apoptotic function of HuROur current work demonstrates that the non-covalent PARylation of HuR plays a key role in regulating its pro-apoptotic function by preventing its cytoplasmic localization and cleavage.
Genetic reprogramming with stem cells regenerates glomerular epithelial podocytes in Alport syndromePodocytes are the rate-limiting glomerular cells for type IV collagen production, and horizontal gene transfer or cell fusion with stem cells regenerates the renal parenchyma in Alport syndrome.
ETV2 induces endothelial, but not hematopoietic, lineage specification in birdsETV2, a master regulator of blood and vessel development in mammals, is deleted in bird genomes, and exogenous ETV2 induces endothelial lineage specification in nascent chicken mesoderm.
Genetic mutation of Cep76 results in male infertility due to abnormal sperm tail compositionThis study explores the role of CEP76 in male fertility via its predicted role in establishing a functional transition zone that mediates protein entry into the sperm tail.
Human CRB1 and CRB2 form homo- and heteromeric protein complexes in the retinaThis study describes novel interactors of the retinal Crumbs complex and reveals homo- and heterotypic interactions of CRB1 and CRB2 that are not significantly affected by patient-associated mutations.
VCP/p97 mediates nuclear targeting of non-ER-imported prion protein to maintain proteostasisThe study shows a novel ubiquitin-independent role of VCP/p97 in the nuclear targeting of non-imported secretory proteins to prevent formation of toxic protein aggregates in the cytosol.
Acetylation of Rec8 cohesin complexes regulates reductional chromosome segregation in meiosisMeikin-dependent acetylation of Rec8 cohesin complexes at centromeres cooperates with the canonical cohesin acetylation to establish reductional chromosome segregation in meiosis in fission yeast.
Differential effects of translation inhibitors on Plasmodium berghei liver stage parasitesFive diverse translation inhibitors are tested at equivalent effective concentrations, revealing that translation inhibition efficacy does not determine the Plasmodium berghei liver stage antiplasmodial efficacy.
Sensitive circulating tumor DNA–based residual disease detection in epithelial ovarian cancerTumor-guided circulating tumor DNA analysis achieves high sensitivity and specificity for post-operative surveillance of epithelial ovarian cancer, including the first test of assays targeting thousands of mutations.
DEFA1A3 DNA gene-dosage regulates the kidney innate immune response during upper urinary tract infectionα-Defensin 1-3 (DEFA1A3) are host antimicrobial peptides with potent innate immune functions during infectious diseases. Differential UTI risk has been linked to DEFA1A3 DNA polymorphisms. This study elucidates mechanisms of DEFA1A3 gene dose–dependent protection against UTI pathogenesis.
Structural basis of translation inhibition by a valine tRNA-derived fragmentWe analyzed three structures of Val-tRF-30S ribosomal complexes, which revealed the mechanism by which val-tRF inhibits protein translation. The mechanism includes the binding of val-tRNA to the decoding site, inhibiting the binding of tRNA-mRNA, and inhibiting the binding of aIF1A.
Subdomains of the Helicobacter pylori Cag T4SS outer membrane core complex exhibit structural independenceStructural and proteomic analyses of H. pylori Cag T4SSs purified from deletion mutants highlight the unexpected structural independence between the OMC and PR, two major subdomains of this complex.
Methods
Generation of marmoset primordial germ cell–like cells under chemically defined conditionsMarmoset primordial germ cells (PGCs) express SOX17, AP2Ɣ, and BLIMP1 in vivo, and under specific feeder-free conditions, marmoset iPSCs can be induced into PGC-like cells in vitro.
Resources
A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sitesA genetic screen in Drosophila photoreceptors uncovers multiple regulators of lipid transfer function and endoplasmic reticulum–plasma membrane contact sites.
Reverse-engineering the anti-MUC1 antibody 139H2 by mass spectrometry–based de novo sequencingA widely used anti-MUC1 antibody (139H2) is sequenced by mass spectrometry, revealing the molecular basis of its glycosylation-independent binding to this tumor-associated antigen.
Genetic structure and diversity of the rfb locus of pathogenic species of the genus LeptospiraThis study provides a broad investigation of the gene composition of the rfb locus, the main genetic factor associated with serology in Leptospira, and opens the way for molecular diagnostics in leptospirosis.
Comprehensive meta-analysis reveals distinct gene expression signatures of MASLD progressionMeta-analysis of hepatic gene expression reveals novel drivers of progression in metabolic dysfunction-associated liver disease (MASLD).
Corrections
Correction: The pyruvate dehydrogenase complex regulates mitophagic trafficking and protein phosphorylationMutations in the PDC affect the phosphorylation and mitophagic trafficking of matrix proteins, through the novel regulation of associated kinases and a phosphatase. We suggest that this occurs by the direct allosteric regulation of the phosphatase and kinases by the PDC.
