Table of Contents
Reviews
Cholesterol and COVID-19—therapeutic opportunities at the host/virus interface during cell entryThe key to cholesterol-related broad-spectrum antiviral strategies may be to disrupt cholesterol handling in specific cellular organelles.
Research Articles
Oxidative phosphorylation is a pivotal therapeutic target of fibrodysplasia ossificans progressivaOxidative phosphorylation plays a pivotal role in heterotopic ossification associated with fibrodysplasia ossificans progressiva, underscoring its potential as a significant therapeutic target.
IFT88 maintains sensory function by localising signalling proteins along Drosophila ciliaDmIFT88 is dispensable to preserve the auditory ciliary structure but is critical to maintain sensory cilia function through binding and localisation of the ciliary proteins Iav and DmGucy2d.
The RNA-binding protein Msi2 regulates autophagy during myogenic differentiationThis study identifies the RNA-binding protein Musashi2 as a novel regulator of myogenesis. Msi2 regulates myoblast differentiation via controlling autophagic protein LC3 expression. Loss of Msi2 results in defective skeletal muscle function in vivo.
Breast implant surface topography triggers a chronic-like inflammatory responseThis study explores the impact of breast implant surface topographies on immune responses, using analyses of periprosthetic fluids and in vitro cultures on model surface replicas, revealing that macrotextured implants significantly induce chronic-like inflammatory reactions.
SHIP1 deficiency causes inflammation-dependent retardation in skeletal growthSHIP1 plays a cell-intrinsic role in osteoclast function and development, yet skeletal growth retardation in SHIP1-deficient mice is dependent on lymphocyte-induced inflammation.
Galectin-8 modulates human osteoclast activity partly through isoform-specific interactionsGalectin-8 is emerging as a key player in osteoclastic bone resorption. Explore the specific functions of its isoforms through interactome analysis, revealing intricate mechanisms of bone biology.
Metabolic and circadian inputs encode anticipatory biogenesis of hepatic fed microRNAsUnravelling molecular and physiological mechanisms that couple circadian and metabolic cues to regulate anticipatory miR biogenesis during fed-fast-refed cycles in the liver.
Lipotype acquisition during neural development is not recapitulated in stem cell–derived neuronsWe report a time-resolved resource of lipid molecule abundance across brain development. Using the resource as a reference, we find that stem cell–derived neurons do not acquire a neural lipotype in vitro, unless supplemented with adequate metabolic precursors.
Stable structures or PABP1 loading protects cellular and viral RNAs against ISG20-mediated decayIn this work, two novel elements that modulate the susceptibility of target RNAs to degradation by the antiviral RNase ISG20 are described.
Pip5k1γ regulates axon formation by limiting Rap1 activityPip5k1γ is shown to regulate axon formation as a negative feedback signal. Impairing its function or manipulating membrane tension by osmotic shock induces the formation of supernumerary axons by hyper-activation of C3G and Rap1.
Altered myocardial lipid regulation in junctophilin-2–associated familial cardiomyopathiesOur studies revealed, for the first time, unique alterations in the cardiac lipid composition—including distinct increases in neutral lipids and decreases in polar membrane lipids—in mice with HCM and DCM were caused by distinct JPH2 variants.
Systemic metabolic depletion of gut microbiome undermines responsiveness to melanoma immunotherapyRelationship between gut microbiome composition and immunotherapy efficacy.
Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis IPhosphorylation of the Rec8 cohesin complexes plays a crucial role in establishing mono-orientation of sister kinetochores which is important for reductional division in meiosis I in fission yeast.
Oncogenic mutations of KRAS modulate its turnover by the CUL3/LZTR1 E3 ligase complexThe study reveals how KRAS mutations disrupt LZTR1-mediated KRAS degradation, shedding light on potential mechanisms in oncogenesis.
Tight regulation of a nuclear HAPSTR1-HUWE1 pathway essential for mammalian lifeThe recently discovered HAPSTR1 protein facilitates a nuclear quality control pathway essential for mammalian life.
C5aR plus MEK inhibition durably targets the tumor milieu and reveals tumor cell phagocytosisC5aR antagonist and MEK inhibition combination therapy changes the tumor microenvironment and induces ongoing tumor cell phagocytosis.
Starvation-resistant cavefish reveal conserved mechanisms of starvation-induced hepatic lipotoxicityCavefish evolved protection from starvation-induced liver damage through a reduction in fatty acid uptake regulated by FATP2, a mechanism conserved through 400 million years of animal evolution.
Interrogating endothelial barrier regulation by temporally resolved kinase network generationThis study introduces TREKING, a methodology for broadly interrogating kinases to elucidate time-resolved functional kinase signaling networks that drive endothelial barrier disruption and recovery.
The Golgi checkpoint: Golgi unlinking during G2 is necessary for spindle formation and cytokinesisThis work shows that Golgi complex disassembly during G2 is required for spindle formation and cytokinesis, thus revealing novel regulatory mechanisms that control the fidelity of mitosis.
Resources
Ageing-dependent thiol oxidation reveals early oxidation of proteins with core proteostasis functionsProteome-wide analysis of thiol oxidation during chronological ageing of yeast reveals evolutionarily conserved proteins as early-oxidation targets with a potential impact on proteostasis regulation.
A comparative study of structural variant calling in WGS from Alzheimer’s disease familiesWe developed a flexible protocol to generate a high-quality deletion call set and a truth set of Sanger sequencing–validated deletions with precise breakpoints between 1 and 17,000 bp from whole-genome sequencing data in multiplex families with Alzheimer’s disease.
Corrections
- Correction: Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish
The vast majority (99%) of mitochondrial proteins are encoded by the nuclear genome, synthesized in the cytosol and imported into the organelle. Here we study the principles of mitochondrial import in vivo from the RNA perspective using zebrafish.
