Table of Contents
Research Articles
E. hellem Ser/Thr protein phosphatase PP1 targets the DC MAPK pathway and impairs immune functionsIntracellular pathogen E. hellem suppressed host DCs’ immune functions via pathogen-derived serine/threonine protein phosphatase PP1 directly targeting the host p38α/MAPK pathway.
Nucleolar protein TAAP1/C22orf46 confers pro-survival signaling in non-small cell lung cancerCRISPR/Cas9 screening identifies the gene C22orf46 to counteract pro-apoptotic signals provided by T cells or anti-neoplastic drugs and contributes to tumorigenesis.
Accessory genes define species-specific routes to antibiotic resistanceIn this study, we show how decision tree models can accurately predict the AMR phenotype, and this study highlights that resistance is a result of multi-gene interactions, which are often species-specific.
The LINC complex ensures accurate centrosome positioning during prophaseCentrosome positioning in prophase is essential for efficient spindle assembly. Here, we show that centrosome positioning requires LINC complex–mediated loading of dynein on the nuclear envelope.
Spatiotemporal DNA methylation dynamics shape megabase-scale methylome landscapesThe guanine–cytosine content of genomic regions influences their spatial properties, resulting in various megabase-scale methylome patterns across different cell types.
Reduced myeloid commitment and increased uptake by macrophages of stem cell–derived HPS2 neutrophilsOur current work uses iPSC-derived neutrophils to unveil novel reasons for the neutropenia found in HPS2 patients. HPS2 iPSC-derived neutrophils have lower myeloid commitment, and are actively phagocytosed by macrophages present in the iPSC cultures or healthy donor macrophages.
Absence of the RING domain in MID1 results in patterning defects in the developing human brainMolecular and cellular phenotyping of human brain organoids carrying various MID1 mutations reveals an unexpected role of the composition of the MID1 isoform pool during early patterning processes.
Synaptobrevin2 monomers and dimers differentially engage to regulate the functional trans-SNARE assemblyThis work demonstrates different factors controlling the synaptobrevin2 dimer-to-monomer ratio, which has ramifications in fusion pore stability.
Drosophila MIC10b can polymerize into cristae-shaping filamentsDmMIC10b, the primary MIC10 orthologue in Drosophila, is crucial for MICOS stability and mitochondrial ultrastructure and exhibits a distinctive ability to form filaments, impacting mitochondrial cristae architecture.
Single variant, yet “double trouble”: TSC and KBG syndrome because of a large de novo inversionShort-read genome sequencing identified an 87-Mb inversion in a patient with atypical Tuberous sclerosis. The inversion disrupts TSC2 and ANKRD11, resulting in dual diagnosis of TSC/KBG syndromes.
Integrated analysis of RNA-seq datasets reveals novel targets and regulators of COVID-19 severityThis work observed differential expression of genes related to the endomembrane system, specific granules, T cell receptor complex, and SPI1 and TP53 target genes exclusively in severe COVID-19 patients.
NeoMUST: an accurate and efficient multi-task learning model for neoantigen presentationNeoMUST, a multi-task learning model, efficiently predicts neoantigen presentation via MHC-I molecules, rivaling existing algorithms with significantly shorter training time. Its GitHub repository offers free access for advancing cancer immunotherapy development.
Mapping parental DMRs predictive of local and distal methylome remodeling in epigenetic F1 hybridsWe show that parental DNA methylation divergence in pericentromeric regions predicts non-additivity in the methylomes, transcriptomes, and phenotypes of F1 hybrids, independently of DNA sequence divergence.
A feedback loop that drives cell death and proliferation and its defect in intestinal stem cellsA positive feedback circuit that simultaneously drives cell death and proliferation and its defect in intestinal stem cells in Drosophila were discovered.
Targeting circulating labile heme as a defense strategy against malariaMalaria remains a major cause of human morbidity and mortality. Circulating labile heme is an independent risk factor for severe P. falciparum malaria, suggesting that labile heme may be a therapeutic target against severe malaria.
Zebrafish tsc1 and cxcl12a increase susceptibility to mycobacterial infectionKnockdown of miR-126 increases susceptibility to mycobacterial infection which can be independently reversed by targeting Tsc1/mTOR or ccr2 implicating macrophage function.
Micronucleus is not a potent inducer of the cGAS/STING pathwayThe FuVis2 reporter system, designed to visualize cell nuclei harboring sister chromatid fusion, equipped with cGAS/STING reporters, offers critical insights that micronuclei are primarily captured by cGAS during mitosis but do not lead to STING activation.
Genome-wide CRISPR screen reveals the synthetic lethality between BCL2L1 inhibition and radiotherapyUnbiased whole-genome CRISPR/Cas9 screening reveals that loss of BCL2L1 shows synthetic lethality with radiation, which is further confirmed with the use of BCL2L1 inhibitor. Thus, radiation plus inhibitors targeting BCL2 family proteins is a promising combination therapy for cancers.
Stability of gut microbiome after COVID-19 vaccination in healthy and immuno-compromised individualsThis study highlights the resilience of the gut microbiome to host immune changes triggered by COVID-19 vaccination and suggest minimal, if any, impact on microbiome-mediated processes.
Knockout of PA200 improves proteasomal degradation and myelination in a proteotoxic neuropathyProteasome Activator 200 is upregulated in a neuropathy model in which protein degradation by the proteasome is impaired. Knocking out PA200 unexpectedly rescued proteostasis and prevented neuropathy.
Epigenomic states contribute to coordinated allelic transcriptional bursting in iPSC reprogrammingEpigenomic states are linked to the coordinated allelic transcriptional bursting of genes involved in iPSC reprogramming.
Inflammatory markers for improved recurrent UTI diagnosis in postmenopausal womenHere, we identify urinary cytokines that are differentially abundant in postmenopausal women with symptomatic recurrent UTI compared with controls and evaluate their ability to accurately diagnose UTI.
ADAM19 cleaves the PTH receptor and associates with brachydactyly type EWe studied a family with autosomal-dominant brachydactyly type E and found a mutated ADAM19; we show that ADAM19 cleaves PTHR1 and that aberrant PTHrP signaling could cause the phenotype.
Oral bacteria induce IgA autoantibodies against a mesangial protein in IgA nephropathy model miceThis work shows that the CBX3, atypically expressed on the mesangial cell, is a target of IgA auto-Abs in IgA nephropathy model mice and patients. Such IgA auto-Abs are induced by oral bacteria in mice.
Apical dehydration impairs the cystic fibrosis airway epithelium barrier via a β1-integrin/YAP1 pathwayDehydration of the airway epithelial cell surface, a hallmark of CF, increases plasma membrane tension, resulting in altered Hippo signaling associated with the loss of barrier function.
Variety in the USP deubiquitinase catalytic mechanismQuantitative analysis shows that USP family members have varying catalytic triads. Their third and fourth catalytic residues change in role and importance for nucleophilic attack and catalysis.
Reviews
FERM domain–containing proteins are active components of the cell nucleusThe information we gathered from the literature reveals that FERM domain proteins perform important and diverse functions not only in the cytoplasm, but also in the cell nucleus.
