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A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells

View ORCID ProfileSumana Sharma  Correspondence email, View ORCID ProfileToby Whitehead, View ORCID ProfileMateusz Kotowski, Emily Zhi Qing Ng, View ORCID ProfileJoseph Clarke, Judith Leitner, View ORCID ProfileYi-Ling Chen, View ORCID ProfileAna Mafalda Santos, Peter Steinberger, View ORCID ProfileSimon J Davis  Correspondence email
Sumana Sharma
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Conceptualization, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing—original draft, Project administration, Writing—review and editing
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  • ORCID record for Sumana Sharma
  • For correspondence: sumana.sharma{at}rdm.ox.ac.uk
Toby Whitehead
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Formal analysis, Validation, Investigation, Visualization, Methodology, Writing—review and editing
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  • ORCID record for Toby Whitehead
Mateusz Kotowski
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Investigation, Methodology, Writing—review and editing
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Emily Zhi Qing Ng
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Validation, Investigation, Methodology
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Joseph Clarke
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Methodology
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  • ORCID record for Joseph Clarke
Judith Leitner
2Division of Immune Receptors and T Cell Activation, Institute of Immunology, Medical University of Vienna, Vienna, Austria
Roles: Methodology
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Yi-Ling Chen
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Investigation, Methodology
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  • ORCID record for Yi-Ling Chen
Ana Mafalda Santos
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
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Peter Steinberger
2Division of Immune Receptors and T Cell Activation, Institute of Immunology, Medical University of Vienna, Vienna, Austria
Roles: Investigation, Methodology, Writing—review and editing
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Simon J Davis
1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
Roles: Conceptualization, Supervision, Funding acquisition, Writing—review and editing
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  • For correspondence: simon.davis{at}imm.ox.ac.uk
Published 7 December 2023. DOI: 10.26508/lsa.202302359
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Article Information

vol. 7 no. 3 e202302359
DOI 
https://doi.org/10.26508/lsa.202302359
PubMed 
38073578

Published By 
Life Science Alliance
Online ISSN 
2575-1077
History 
  • Received September 8, 2023
  • Revision received November 30, 2023
  • Accepted November 30, 2023
  • Published online December 7, 2023.

Copyright & Usage 
© 2023 Sharma et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

Author Information

  1. Sumana Sharma1,*, Conceptualization, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing—original draft, Project administration, Writing—review and editing⇑,
  2. Toby Whitehead1,*, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing—review and editing,
  3. Mateusz Kotowski1, Investigation, Methodology, Writing—review and editing,
  4. Emily Zhi Qing Ng1, Validation, Investigation, Methodology,
  5. Joseph Clarke1, Methodology,
  6. Judith Leitner2, Methodology,
  7. Yi-Ling Chen1, Investigation, Methodology,
  8. Ana Mafalda Santos1, Investigation, Methodology,
  9. Peter Steinberger2, Investigation, Methodology, Writing—review and editing and
  10. Simon J Davis1, Conceptualization, Supervision, Funding acquisition, Writing—review and editing⇑
  1. 1MRC Translational Immune Discovery Unit, John Radcliffe Hospital, University of Oxford, Oxford, UK
  2. 2Division of Immune Receptors and T Cell Activation, Institute of Immunology, Medical University of Vienna, Vienna, Austria
  1. Correspondence: sumana.sharma{at}rdm.ox.ac.uk
  2. simon.davis{at}imm.ox.ac.uk
  1. ↵* Sumana Sharma and Toby Whitehead contributed equally to this work

View Full Text

Funding

  • Wellcome Trust (WT)

    215883/Z/19/Z
    S Sharma
  • Wellcome Trust (WT)

    207547/Z/17/Z
    SJ Davis
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Inhibitory signal screening in T cells
Sumana Sharma, Toby Whitehead, Mateusz Kotowski, Emily Zhi Qing Ng, Joseph Clarke, Judith Leitner, Yi-Ling Chen, Ana Mafalda Santos, Peter Steinberger, Simon J Davis
Life Science Alliance Dec 2023, 7 (3) e202302359; DOI: 10.26508/lsa.202302359

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Inhibitory signal screening in T cells
Sumana Sharma, Toby Whitehead, Mateusz Kotowski, Emily Zhi Qing Ng, Joseph Clarke, Judith Leitner, Yi-Ling Chen, Ana Mafalda Santos, Peter Steinberger, Simon J Davis
Life Science Alliance Dec 2023, 7 (3) e202302359; DOI: 10.26508/lsa.202302359
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Volume 7, No. 3
March 2024
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