Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m5C at GCU motifs

Kaylee J Watson; Robin E Bromley; Benjamin C Sparklin; Mark T Gasser; Tamanash Bhattacharya; Jarrett F Lebov; Tyonna Tyson; Nan Dai; Laura E Teigen; Karen T Graf; Jeremy M Foster; Michelle Michalski; Vincent M Bruno; Amelia RI Lindsey; Ivan R Corrêa; Richard W Hardy; Irene LG Newton; Julie C Dunning Hotopp

doi:10.26508/lsa.202302201

Research Article

Open Access

Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m⁵C at GCU motifs

View ORCID ProfileKaylee J Watson, View ORCID ProfileRobin E Bromley, View ORCID ProfileBenjamin C Sparklin, Mark T Gasser, Tamanash Bhattacharya, Jarrett F Lebov, Tyonna Tyson, Nan Dai, Laura E Teigen, Karen T Graf, Jeremy M Foster, Michelle Michalski, Vincent M Bruno, View ORCID ProfileAmelia RI Lindsey, Ivan R Corrêa, View ORCID ProfileRichard W Hardy, Irene LG Newton, View ORCID ProfileJulie C Dunning Hotopp Correspondence email

Kaylee J Watson

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Conceptualization, Software, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing—original draft, Writing—review and editing

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Kaylee J Watson

Robin E Bromley

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Data curation, Investigation, Methodology, Writing—original draft, Project administration

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Robin E Bromley

Benjamin C Sparklin

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Benjamin C Sparklin

Mark T Gasser

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Tamanash Bhattacharya

2Department of Biology, Indiana University, Bloomington, IN, USA

Roles: Resources, Investigation, Methodology, Writing—original draft

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Jarrett F Lebov

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Tyonna Tyson

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Nan Dai

3New England Biolabs, Ipswich, MA, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Laura E Teigen

4Department of Biology, University of Wisconsin Oshkosh, Oshkosh, WI, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Karen T Graf

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Investigation, Writing—original draft

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Jeremy M Foster

3New England Biolabs, Ipswich, MA, USA

Roles: Resources, Supervision

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Michelle Michalski

4Department of Biology, University of Wisconsin Oshkosh, Oshkosh, WI, USA

Roles: Resources, Supervision

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Vincent M Bruno

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

5Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Resources, Supervision

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Amelia RI Lindsey

2Department of Biology, Indiana University, Bloomington, IN, USA

Roles: Resources, Supervision, Investigation, Methodology, Writing—review and editing

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Amelia RI Lindsey

Ivan R Corrêa Jr

3New England Biolabs, Ipswich, MA, USA

Roles: Resources, Investigation, Methodology

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Richard W Hardy

2Department of Biology, Indiana University, Bloomington, IN, USA

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Investigation, Project administration, Writing—review and editing

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Richard W Hardy

Irene LG Newton

2Department of Biology, Indiana University, Bloomington, IN, USA

Roles: Conceptualization, Resources, Supervision, Funding acquisition, Investigation, Project administration, Writing—review and editing

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Julie C Dunning Hotopp

1Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA

5Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA

6Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, USA

Roles: Conceptualization, Data curation, Supervision, Funding acquisition, Investigation, Methodology, Writing—original draft, Project administration, Writing—review and editing

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Julie C Dunning Hotopp
For correspondence: jdhotopp@som.umaryland.edu

Published 29 November 2023. DOI: 10.26508/lsa.202302201

Article
Figures & Data
Info
Metrics
Reviewer Comments
PDF

You are using an old browser, click here to download PDF

PDF Container

PreviousNext

Download PDF

Email Article

Thank you for your interest in spreading the word on Life Science Alliance.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Your Email *

Your Name *

Send To *

Enter multiple addresses on separate lines or separate them with commas.

You are going to email the following Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m5C at GCU motifs

Message Subject (Your Name) has sent you a message from Life Science Alliance

Message Body (Your Name) thought you would like to see the Life Science Alliance web site.

Your Personal Message

CAPTCHA

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

Citation Tools

Misidentification of m⁵C at GCU in ONT direct RNA sequencing

Kaylee J Watson, Robin E Bromley, Benjamin C Sparklin, Mark T Gasser, Tamanash Bhattacharya, Jarrett F Lebov, Tyonna Tyson, Nan Dai, Laura E Teigen, Karen T Graf, Jeremy M Foster, Michelle Michalski, Vincent M Bruno, Amelia RI Lindsey, Ivan R Corrêa, Richard W Hardy, Irene LG Newton, Julie C Dunning Hotopp

Life Science Alliance Nov 2023, 7 (2) e202302201; DOI: 10.26508/lsa.202302201

Citation Manager Formats

BibTeX
Bookends
EasyBib
EndNote (tagged)
EndNote 8 (xml)
Medlars
Mendeley
Papers
RefWorks Tagged
Ref Manager
RIS
Zotero

Misidentification of m⁵C at GCU in ONT direct RNA sequencing

Life Science Alliance Nov 2023, 7 (2) e202302201; DOI: 10.26508/lsa.202302201

Share This Article:

Tweet Widget

In this Issue

Volume 7, No. 2

February 2024

Table of Contents
Cover (PDF)
About the Cover
Masthead (PDF)

Jump to section

Article
Figures & Data
Info
Metrics
Reviewer Comments
PDF

Subjects

Genomics & Functional Genomics

No related articles found.

Cited By...

No citing articles found.

Google Scholar

Main menu

User menu

Search

Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m⁵C at GCU motifs

Citation Manager Formats

In this Issue

Subjects

Related Articles

Cited By...

More in this TOC Section

Similar Articles

Content

For Authors

Other Services

More Information