CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane

Martin Ondra; Lukas Lenart; Amanda Centorame; Daciana C Dumut; Alexander He; Syeda Sadaf Zehra Zaidi; John W Hanrahan; Juan Bautista De Sanctis; Danuta Radzioch; Marian Hajduch

doi:10.26508/lsa.202302045

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CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane

View ORCID ProfileMartin Ondra, Lukas Lenart, Amanda Centorame, Daciana C Dumut, Alexander He, Syeda Sadaf Zehra Zaidi, View ORCID ProfileJohn W Hanrahan, View ORCID ProfileJuan Bautista De Sanctis, View ORCID ProfileDanuta Radzioch Correspondence email, View ORCID ProfileMarian Hajduch Correspondence email

Martin Ondra

1Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

4Czech Advanced Technology and Research Institute, Palacky University, Olomouc, Czech Republic

Roles: Data curation, Formal analysis, Investigation, Methodology, Writing—original draft, Writing—review and editing

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ORCID record for Martin Ondra

Lukas Lenart

1Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

Roles: Investigation, Methodology, Writing—original draft, Writing—review and editing

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Amanda Centorame

2Faculty of Medicine and Health Sciences, McGill University, Montreal, Canada

3RI-MUHC, Montreal, Canada

Roles: Investigation, Writing—original draft, Writing—review and editing

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Daciana C Dumut

2Faculty of Medicine and Health Sciences, McGill University, Montreal, Canada

3RI-MUHC, Montreal, Canada

Roles: Investigation, Writing—original draft, Writing—review and editing

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Alexander He

5Physiology, McGill University, Montreal, Canada

Roles: Investigation

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Syeda Sadaf Zehra Zaidi

5Physiology, McGill University, Montreal, Canada

Roles: Investigation

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John W Hanrahan

3RI-MUHC, Montreal, Canada

5Physiology, McGill University, Montreal, Canada

Roles: Investigation

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ORCID record for John W Hanrahan

Juan Bautista De Sanctis

1Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

Roles: Methodology, Writing—original draft, Writing—review and editing

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ORCID record for Juan Bautista De Sanctis

Danuta Radzioch

1Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

2Faculty of Medicine and Health Sciences, McGill University, Montreal, Canada

3RI-MUHC, Montreal, Canada

Roles: Conceptualization, Supervision, Investigation, Writing—original draft, Writing—review and editing

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ORCID record for Danuta Radzioch
For correspondence: danuta.radzioch@mcgill.ca

Marian Hajduch

1Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic

4Czech Advanced Technology and Research Institute, Palacky University, Olomouc, Czech Republic

6Laboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, University Hospital Olomouc, Olomouc, Czech Republic

Roles: Conceptualization, Data curation, Supervision, Funding acquisition, Investigation, Methodology, Writing—original draft, Writing—review and editing

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ORCID record for Marian Hajduch
For correspondence: marian.hajduch@upol.cz

Published 2 November 2023. DOI: 10.26508/lsa.202302045

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Luminescence assay for monitoring CFTR trafficking

Martin Ondra, Lukas Lenart, Amanda Centorame, Daciana C Dumut, Alexander He, Syeda Sadaf Zehra Zaidi, John W Hanrahan, Juan Bautista De Sanctis, Danuta Radzioch, Marian Hajduch

Life Science Alliance Nov 2023, 7 (1) e202302045; DOI: 10.26508/lsa.202302045

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Luminescence assay for monitoring CFTR trafficking

Martin Ondra, Lukas Lenart, Amanda Centorame, Daciana C Dumut, Alexander He, Syeda Sadaf Zehra Zaidi, John W Hanrahan, Juan Bautista De Sanctis, Danuta Radzioch, Marian Hajduch

Life Science Alliance Nov 2023, 7 (1) e202302045; DOI: 10.26508/lsa.202302045

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