Table of Contents
Research Articles
Vitamin C protects retinal ganglion cells via SPP1 in glaucoma and after optic nerve damageVitamin C protects retinal ganglion cells in animal models of optic nerve injury partially by up-regulating the expression of the neuroprotective signaling molecule SPP1.
Methylation of lysine 36 on histone H3 is required to control transposon activities in somatic cellsDisruption of H3K36 methylation in somatic cells leads to transposon transcription and activation of key genes in the PIWI-interacting RNA pathway together with RNA production from piRNA clusters.
Antibody reliability influences observed mRNA–protein correlations in tumour samplesUpadhya and Ryan show that the reliability of antibodies used for RPPA profiling influences the mRNA-protein correlations observed in tumour cohorts.
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelopeThe LINC complex tethers the cell nucleus to the cytoskeleton to regulate mechanical forces during cell migration, differentiation, and various diseases. We provide evidence for SUN2 disulfide bond rearrangement as a physiologically relevant structural modification that regulates LINC complex functions.
FAT10 and NUB1L cooperate to activate the 26S proteasome26S proteasome activation is achieved by the collective binding of the ubiquitin-like modifier FAT10 and its interaction partner NUB1L, causing gate opening in a USP14-independent way.
Identification of a novel enhancer essential for Satb1 expression in TH2 cells and activated ILC2sThe transcriptional mechanisms for Satb1 gene expression are ill-defined. This study identifies a novel enhancer for Satb1 expression in TH2 cells and to suppress IL-5 production, and for Satb1 expression in activated ILC2s.
HR repair pathway plays a crucial role in maintaining neural stem cell fate under irradiation stressMRN complex and the HR repair pathway play a crucial role in the maintenance of neural stem cell fate under irradiation stress.
Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrestElevated Cx37 expression in endothelium stabilizes nuclear Foxo3a by sequestering active ERK in the cytoplasm, thereby up-regulating p27 expression to promote late G1 state and enable arterial gene expression.
WDR31 displays functional redundancy with GTPase-activating proteins (GAPs) ELMOD and RP2 in regulating IFT complex and recruiting the BBSome to ciliumOur research reveals that the deletion of WDR-31, RP-2, and ELMD-1 is leading to ciliary accumulation of IFT Complex B components and KIF17 kinesin. In conclusion, WDR31, ELMOD, and RP2 are working in regulating IFT via the BBSome.
Expression of TMPRSS2 is up-regulated by bacterial flagellin, LPS, and Pam3Cys in human airway cellsThe expression of the virus-activating protease TMPRSS2 in airway cells is enhanced by bacterial components. This may contribute to virus activation and replication in co-infections of IAV and bacteria.
Origin and segregation of the human germlineHuman primordial germ cells originate in peri-gastrulation embryos from the posterior epiblast precursors with an obligatory role for TFAP2A, which is redundant for contribution to the amnion.
MAP4K3 inhibits Sirtuin-1 to repress the LKB1–AMPK pathway to promote amino acid-dependent activation of the mTORC1 complexOur results reveal the existence of a novel signaling pathway linking amino acid satiety with MAP4K3-dependent suppression of SIRT1 to inactivate the repressive LKB1–AMPK pathway and thereby potently activate the mTORC1 complex to dictate the metabolic disposition of the cell.
The CHARGE syndrome-associated protein FAM172A controls AGO2 nuclear importAGO2 has long been known to shuttle between the cytosol and the nucleus of mammalian cells and this study helps to understand how this occurs.
Inter and transgenerational impact of H3K4 methylation in neuronal homeostasisThe study highlights the importance of maintaining appropriate H3K4me3 levels to prevent negative impacts on nerve system homeostasis across generations.
UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryosThis study shows that the UHRF1 protein, an epigenetic factor, regulates cytoplasmic proteins in oocytes independently of gene expression and ensures normal preimplantation development.
Modeling fibrotic alveolar transitional cells with pluripotent stem cell-derived alveolar organoidsIn vitro modeling of transitional alveolar epithelial cells in idiopathic pulmonary fibrosis by the use of a fibrosis cocktail and alveolar organoids from induced pluripotent stem cells.
A new metabolic model of Drosophila melanogaster and the integrative analysis of Parkinson’s diseaseThe reconstruction of a comprehensive and literature-curated Drosophila metabolic model, iDrosophila1, provided a powerful platform for studying metabolism with systems medicine perspective.
Amphid sensory neurons of Caenorhabditis elegans orchestrate its survival from infection with broad classes of pathogensThis study shows that the nervous system of the bacterivorous nematode Caenorhabditis elegans coordinates pathogen-specific and broad immune responses during infection.
Inhibition of microRNA-33b in humanized mice ameliorates nonalcoholic steatohepatitisExperiments using humanized mice created by knocking in microRNA (miR)-33b into an intron of Srebf1 show that suppression of miR-33b by nucleic acid drugs is useful for the treatment of NASH.
STING is redundant for host defense and pathology of COVID-19-like disease in miceSTING-deficient mice infected with SARS-CoV-2 do not show altered disease development or viral load, nor any major differences in inflammatory responses.
Missense variant interaction scanning reveals a critical role of the FERM domain for tumor suppressor protein NF2 conformation and functionDeep mutational interaction perturbation scanning of NF2 with conformation-dependent interaction partners KDM1A, EMILIN, and PIK3R3 revealed two regions critical for NF2 tumor suppressor function.
Biallelic structural variations within FGF12 detected by long-read sequencing in epilepsyThis study demonstrates biallelic intragenic structural variations and a homozygous single-nucleotide variant in FGF12 cause epilepsy as a new loss-of-function mechanism of FGF12 in humans.
Preeclampsia promotes autism in offspring via maternal inflammation and fetal NFκB signalingThis study highlights elevated TNFα in maternal serum contributes to autism-like phenotypes in offspring exposed to preeclampsia and gives a therapeutic target to prevent it.
Apolipoprotein E intersects with amyloid-β within neuronsApolipoprotein E4, the most important genetic risk factor for Alzheimer's disease, is shown to internalize into neurons and intersect with amyloid-β in endosomes–autophagosomes of neurites and modulate intraneuronal amyloid-β-42.
SLIT2/ROBO1 signaling suppresses mTORC1 for organelle control and bacterial killingSLIT2, a canonical neurorepellent, promotes lysosome biogenesis and augments autophagic flux to modulate immune responses in macrophages. In cancer cells, SLIT2 negatively regulates mTORC1 signaling to control cell growth.
N-terminal proteoforms may engage in different protein complexesProteins originating from the same gene, yet differing at their N-terminus—so-called N-terminal proteoforms—can take part in different protein–protein interactions.
Resources
Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling networkThe site-specific function of EGFR’s C-terminal tyrosines is interrogated by phosphotyrosine phosphoproteomics to build a data-driven model that links cell response to signaling nodes and elucidates novel associations in the EGFR signaling network.
Methods
Evaluation of genetic demultiplexing of single-cell sequencing data from model speciesThe authors demonstrate that scRNA-seq sample pooling followed by genetics-based separation of individuals is an effective means to identify individual samples in a variety of commonly studied species.
Spatial metabolomics for symbiotic marine invertebratesMass spectrometry imaging revealed in situ spatial metabolite distribution patterns at 50 μm resolution for sea anemones and scleractinian corals and their microalgal endosymbionts.
An immune-based tool platform for in vivo cell clearanceA tool platform that allows the exploration of immune-mediated cytotoxic therapies to target pathological cells in diseases beyond cancer.
