Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Methods & Resources
    • Archive
    • Subjects
  • Collections
  • Submit
    • Submit a Manuscript
    • Author Guidelines
    • License, Copyright, Fee
    • FAQ
    • Why submit
  • About
    • About Us
    • Editors & Staff
    • Board Members
    • Licensing and Reuse
    • Reviewer Guidelines
    • Privacy Policy
    • Advertise
    • Contact Us
    • LSA LLC
  • Alerts
  • Other Publications
    • EMBO Press
    • The EMBO Journal
    • EMBO reports
    • EMBO Molecular Medicine
    • Molecular Systems Biology
    • Rockefeller University Press
    • Journal of Cell Biology
    • Journal of Experimental Medicine
    • Journal of General Physiology
    • Cold Spring Harbor Laboratory Press
    • Genes & Development
    • Genome Research

User menu

  • My alerts

Search

  • Advanced search
Life Science Alliance
  • Other Publications
    • EMBO Press
    • The EMBO Journal
    • EMBO reports
    • EMBO Molecular Medicine
    • Molecular Systems Biology
    • Rockefeller University Press
    • Journal of Cell Biology
    • Journal of Experimental Medicine
    • Journal of General Physiology
    • Cold Spring Harbor Laboratory Press
    • Genes & Development
    • Genome Research
  • My alerts
Life Science Alliance

Advanced Search

  • Home
  • Articles
    • Newest Articles
    • Current Issue
    • Methods & Resources
    • Archive
    • Subjects
  • Collections
  • Submit
    • Submit a Manuscript
    • Author Guidelines
    • License, Copyright, Fee
    • FAQ
    • Why submit
  • About
    • About Us
    • Editors & Staff
    • Board Members
    • Licensing and Reuse
    • Reviewer Guidelines
    • Privacy Policy
    • Advertise
    • Contact Us
    • LSA LLC
  • Alerts
  • Follow lsa Template on Twitter
Research Article
Transparent Process
Open Access

Crystal structures of dimeric and heptameric mtHsp60 reveal the mechanism of chaperonin inactivation

Meng-Cheng Lai, View ORCID ProfileHao-Yu Cheng, Sin-Hong Lew, Yu-An Chen, Chien-Hung Yu, Han-You Lin, View ORCID ProfileShih-Ming Lin  Correspondence email
Meng-Cheng Lai
1https://ror.org/01b8kcc49Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan
Roles: Data curation, Formal analysis, Writing—original draft
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hao-Yu Cheng
1https://ror.org/01b8kcc49Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan
Roles: Data curation, Formal analysis
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hao-Yu Cheng
Sin-Hong Lew
1https://ror.org/01b8kcc49Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan
Roles: Data curation, Formal analysis
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yu-An Chen
1https://ror.org/01b8kcc49Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan
Roles: Data curation, Software, Formal analysis
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chien-Hung Yu
2https://ror.org/01b8kcc49Department of Biochemistry and Molecular Biology, National Cheng Kung University, Tainan, Taiwan
5Institute of Basic Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
Roles: Conceptualization, Resources, Writing—review and editing
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Han-You Lin
3Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan
Roles: Conceptualization, Methodology, Writing—review and editing
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shih-Ming Lin
1https://ror.org/01b8kcc49Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan
4https://ror.org/01b8kcc49Institute of Tropical Plant Sciences and Microbiology, National Cheng Kung University, Tainan, Taiwan
Roles: Conceptualization, Supervision, Writing—original draft, Writing—review and editing
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Shih-Ming Lin
  • For correspondence: smlin@mail.ncku.edu.tw
Published 27 March 2023. DOI: 10.26508/lsa.202201753
  • Article
  • Figures & Data
  • Info
  • Metrics
  • Reviewer Comments
  • PDF
Loading

Abstract

Mitochondrial Hsp60 (mtHsp60) plays a crucial role in maintaining the proper folding of proteins in the mitochondria. mtHsp60 self-assembles into a ring-shaped heptamer, which can further form a double-ring tetradecamer in the presence of ATP and mtHsp10. However, mtHsp60 tends to dissociate in vitro, unlike its prokaryotic homologue, GroEL. The molecular structure of dissociated mtHsp60 and the mechanism behind its dissociation remain unclear. In this study, we demonstrated that Epinephelus coioides mtHsp60 (EcHsp60) can form a dimeric structure with inactive ATPase activity. The crystal structure of this dimer reveals symmetrical subunit interactions and a rearranged equatorial domain. The α4 helix of each subunit extends and interacts with its adjacent subunit, leading to the disruption of the ATP-binding pocket. Furthermore, an RLK motif in the apical domain contributes to stabilizing the dimeric complex. These structural and biochemical findings provide new insights into the conformational transitions and functional regulation of this ancient chaperonin.

Introduction

Heat shock protein 60 (Hsp60), also known as chaperonin, is a widely conserved gene found in all living kingdoms (Zeilstra-Ryalls et al, 1991; Gupta, 1995; Bukau & Horwich, 1998; Zhao & Liu, 2017). Chaperonins play a crucial role in assisting protein folding and preventing protein aggregation in cells. There are two groups of chaperonins: type I, found in bacteria, mitochondria, and chloroplasts, and type II, found in eukaryotic cytosol and archaea (Llorca et al, 1999; Horwich et al, 2007; Vilasi et al, 2017). Mitochondrial Hsp60 (mtHsp60), a type I chaperonin, functions in the mitochondria with its co-chaperonin, mtHsp10 (Cheng et al, 1989; Ostermann et al, 1989). Several proteins critical for mitochondrial function are imported as unfolded single polypeptides and require folding with the assistance of mtHsp60 (Hartl, 1996; Frydman, 2001). One such protein is the F-type ATPase, which is crucial for ATP synthesis (Prasad et al, 1990). In addition, mtHsp60 also helps mitochondrial proteins maintain proper folding without being denatured by the highly oxidative environment (Horwich et al, 1999; Voth & Jakob, 2017). As a result, mtHsp60 plays a vital role in maintaining mitochondrial functions in cells.

mtHsp60 is translated in cytosol as a precursor, called naïve mtHsp60, with a mitochondrial importing signal (MIS) at the N-terminus of 26 amino acids. Upon sorting to the mitochondria, the MIS sequence is removed, and naïve mtHsp60 matures into a 60 kD single polypeptide (Singh et al, 1990). mtHsp60 can self-assemble into a ring-shaped heptamer with seven identical protomers (Klebl et al, 2021; Wang & Chen, 2021). In the presence of mtHsp10 and ATP, these heptamers could stack back-to-back to form a double-ring tetradecamer (Nisemblat et al, 2015; Gomez-Llorente et al, 2020). Both single-ring and double-ring mtHsp60 complexes have ATP-dependent folding activities, suggesting that heptameric mtHsp60 is an active oligomeric state (Nielsen & Cowan, 1998; Gomez-Llorente et al, 2020).

The crystal structure of mtHsp60 reveals three domains: apical, intermediate, and equatorial domains (Nisemblat et al, 2015). The apical domain is a single segment that interacts with mtHsp10 and recruits unfolded polypeptides for the folding process. The intermediate domain connects the equatorial and apical domains and provides flexibility for mtHsp60’s conformational changes. The equatorial domain connects the subunits in the heptameric ring and facilitates interactions between the two rings of tetradecamers. In addition, the equatorial domain contributes to the formation of the ATP-binding pocket, which involves the DGTTT motif (85DGTTT89) and residues G414 and D494 (Gomez-Llorente et al, 2020). These residues are known to participate in ATP binding in Escherichia coli GroEL and human mtHsp60 (Boisvert et al, 1996; Nisemblat et al, 2015; Gomez-Llorente et al, 2020). Upon ATP binding, mtHsp60 undergoes a conformational change that enables it to interact with mtHsp10 and assist in protein folding activity.

In addition to the heptameric and tetradecameric complex, various oligomeric states of mtHsp60 have also been reported, including octamer, dimer, and monomer (Klebl et al, 2021). Unlike other type I chaperonin homologs in bacterial, chloroplast, and yeast mitochondria, mammalian mtHsp60 tends to dissociate into monomers during purification and is relatively unstable, whereas stable tetradecamers can be purified from other homologs (Viitanen et al, 1998; Levy-Rimler et al, 2001). A recent study suggests that mtHsp60 has shorter inter-subunit β-strands and unique residues at the inter-subunit interface, which decrease complex association and subunit interactions (Wang & Chen, 2021). Monomeric mtHsp60 is unable to hydrolyze ATP and loses its folding function (Viitanen et al, 1998). However, inactive monomeric mtHsp60 can reconstitute into oligomers by incubating with Mg-ATP. The biochemical characteristics and physiological functions of these dissociated mtHsp60 are not fully understood, and the molecular structure of mtHsp60 in its monomeric or dimeric form has also not been reported.

To address these gaps in knowledge, we cloned and expressed the mtHsp60 gene, HSPD1, from grouper fish (Epinephelus coioides) for biochemical and structural analysis. Our results showed that the E. coioides mtHsp60 (EcHsp60) forms dimers that are incapable of hydrolyzing ATP but can reassemble into heptamers, thus restoring ATPase activity. Furthermore, we reported the crystal structure of dimeric naïve EcHsp60, which reveals a disrupted ATP-binding pocket. Based on these results, we proposed a potential role for dimeric EcHsp60 in elucidating the conformational changes that occur in this vital chaperonin.

Results

Both the naïve and mature EcHsp60 formed a dimeric conformation after purification at low temperature

The stability and oligomeric state of mtHsp60 can be influenced by various factors, including protein concentration, the presence of nucleotides, and the existence of MIS (Levy-Rimler et al, 2001; Vilasi et al, 2014; Ricci et al, 2016). In addition, previous studies have shown that heptameric mtHsp60 can significantly disassemble during purification at low temperatures (Viitanen et al, 1992, 1998). To study the oligomeric forms of EcHsp60, both the naïve and mature forms were expressed and purified at low temperature (LT, 4°C) and room temperature (RT, 25°C), respectively. Size-exclusion chromatography (SEC) was used to analyze the purified proteins, and it was found that both forms of EcHsp60 formed a major peak at a retention volume of 76 ml when purified at low temperature, indicating the formation of dimers (Fig 1A, blue and cyan lines). In contrast, when purified at room temperature, both proteins were predominantly eluted at 63 ml, indicating the formation of larger complexes, possibly heptamers (Fig 1A, red and orange lines). The presence of a shoulder peak only in the mature EcHsp60 purified at room temperature suggests the existence of larger oligomeric forms. These findings suggest that the oligomeric state of EcHsp60 can be affected by the purification temperature and that it can form dimers at low temperatures and heptamers at higher temperatures.

Figure 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1. Both naïve and mature EcHsp60 form dimers when purified at low temperature.

(A) SEC analysis of the naïve and mature EcHsp60 purified under room temperature (RT, red and orange) and 4°C (blue and cyan), respectively. The elution volumes for different sizes of calibration markers are marked as dots on top of the chromatogram. (B) SDS–PAGE and Western blot analysis of the dimers (EcHsp602) and heptamers (EcHsp607) for naïve and mature EcHsp60. (C) Cross-linking analysis of dimeric EcHsp60. Both naïve and mature EcHsp60 in dimeric form were incubated with a series concentration of BS3 and analyzed by SDS–PAGE. The filled triangle indicates the monomer of EcHsp60, whereas open triangle represents the dimeric EcHsp60. (D) Both EcHsp602 and EcHsp607 were used to measure the kinetic curves of ATPase activity at a range of ATP concentration from 3.06–200 μM. Each value represents the average of three independent measurements, and the error bars are standard deviations.

To confirm the protein composition of the major peaks observed in SEC, SDS–PAGE and Western blot analysis were performed (Fig 1B). The results revealed that all major peaks were composed of a single 60 kD polypeptide, indicating that the oligomeric states are composed solely of EcHsp60 without any host proteins. Negative-stain transmission electron microscopy (TEM) analysis further confirmed the heptameric structures of both naïve and mature EcHsp60, as observed in the ring-shaped structures consisting of seven subunits (Fig S1). In addition, cross-linking assays using BS3 as the cross-linker confirmed the dimeric structures of both naïve and mature EcHsp60 (Fig 1C). The dimeric bands increased as the BS3 concentration increased, providing further evidence of the formation of different oligomeric states of EcHsp60 depending on the purification temperature.

Figure S1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S1. Negative-stain TEM analysis and single particle reconstitution of naïve EcHsp607 showed a single-ring heptameric conformation.

(A) The negative-stain micrograph of naïve EcHsp607 acquired at 2,000,00X magnifications. (B) The representative particle images after 2D classification. The position of the subunit is marked with numbers to indicate the heptamer. (C, D) The top (C) and side (D) views of the 3D-reconstituted density map docked with a heptameric human mtHsp60 (PDB: 7AZP). The density map is shown in mesh, whereas the docking coordinate is shown in cartoon and colored cyan.

Dimeric EcHsp60 is deficient in ATPase activity and shows lower stability

To further investigate the functional differences between the various oligomeric states of EcHsp60, the ATPase activity of EcHsp607 and EcHsp602 was measured. The kinetic profiles of both forms were determined over a range of ATP concentrations from 3.06–200 μM. It was found that EcHsp607 was capable of hydrolyzing ATP and its enzymatic kinetics were calculated by fitting a non-linear regression to allosteric sigmoidal equations (Fig 1D). The Khalf and Vmax values are reported in Table S1. The Hill slopes for both curves are 1.5, indicating a positive cooperative effect for EcHsp607. In contrast, neither the naïve nor the mature EcHsp602 showed any ATP hydrolysis activity at any ATP concentration (Fig 1D). These results indicate that EcHsp60 loses its ATPase function when forming a dimeric conformation.

Table S1. Kinetic analysis of the ATPase activity of EcHsp60.

In addition, it was observed that the naïve EcHsp602 degraded more quickly than other EcHsp60 species (Fig 1B), suggesting its lower stability. To assess the protein stability of naïve EcHsp60, a limited trypsin digestion assay was performed. The naïve EcHsp607 and EcHsp602 were incubated with trypsin, and the time-dependent degradation profiles were monitored using SDS–PAGE and Western blot analysis. The results showed that naïve EcHsp607 remained largely unchanged after 60 min of digestion, whereas the naïve EcHsp602 was significantly degraded to 50 kD within 15 min (Fig S2A). The Western blot analysis further confirmed that the C-terminal His-tag was still present, suggesting that the N-terminus of naïve EcHsp602 was degraded within 5 min of trypsinolysis (Fig S2B and C). These results indicate that the naïve EcHsp602 loses its ATPase activity and also reduces its protein stability.

Figure S2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S2. Limited trypsin digestion assay showed that the naïve EcHsp602 is highly sensitive to proteolysis compared with the heptameric form.

(A) The naïve EcHsp607 and EcHsp602 are digested by trypsin for various periods of time and then analyzed by SDS–PAGE. The loading amount is equal for each sample. (B, C) The naïve EcHsp602 was digested by trypsin and sampled every 30 s for (B) SDS–PAGE and (C) Western blot analysis. The primary antibody for Western blot is anti-His antibody. The filled and open triangles indicate the full-length and digested naïve EcHsp60, respectively.

Naïve EcHsp602 could be reconstituted into heptameric complexes with restored ATPase activity

Previous studies have mentioned that the monomeric mtHsp60 can assemble into heptamers in the presence of ATP (Viitanen et al, 1998). To further understand this process, we investigated whether EcHsp602 could be reconstituted into an oligomeric state when ATP was added. The naïve EcHsp602 was incubated with 1 and 2 mM ATP for 30 min at 30°C, and its oligomeric state was monitored by SEC. The results showed that the naïve EcHsp602 reassembled into heptamers in the presence of ATP, with the proportion of heptamers increasing as the ATP concentration was raised (Fig 2A). In addition, the ATP hydrolysis activity was restored in the heptamers assembled from dimers, whereas the unassembled dimers remained inactive in ATP hydrolysis (Fig 2B and C). These findings suggest that the naïve EcHsp602 adopts a transient state with a loss of ATPase function, which can be reactivated into heptamers upon ATP binding.

Figure 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2. Dimeric naïve EcHsp60 could be reconstituted into heptamers with restored ATPase activity.

(A) SEC chromatogram showed the conformational changes of naïve EcHsp602 after incubation with various concentrations of ATP for 30 min at 30°C. (B, C) The naïve EcHsp607 assembled from the naïve EcHsp602 was separated and collected using SEC for measuring the ATPase activity. (B) is the naïve EcHsp602 only; (C) is the naïve EcHsp602 incubated with 4 mM ATP. The naïve EcHsp602 and assembled naïve EcHsp607 were indicated by filled and open triangles, respectively.

Crystal structure of the naïve EcHsp602 showed a twofold symmetric conformation with a reorganized equatorial domain

To understand the molecular basis of the inactivation of dimeric EcHsp60, the crystal structure of the naïve EcHsp602 was solved at a resolution of 2.35 Å (Table S2). This represents the first-ever structure of dimeric mtHsp60. The dimeric structure of the naïve EcHsp60 (EcHsp602) revealed a twofold symmetrical conformation, with two protomers facing each other in an asymmetric unit (Fig 3A). The N-terminal (MIS and residues 1–87) and the C-terminal (residues 508–552) regions of both protomers were not observed in the density maps because of their disordered structure, and only a partial equatorial domain was built in this model (Fig 3A). In contrast, the apical and intermediate domains of naïve EcHsp602 were well defined, except for a few flexible loops. The two protomers had a similar protein structure with a Cα root-mean-square deviation of 1.54 Å (Fig 3B). The primary structural variation between the two protomers is located at an α-helix section (T88-S106), which is also known as the fourth α-helix in the full-length chaperonin and will be referred to as α4 in the following discussion (Fig S3). The equatorial domain of the naïve EcHsp602 showed greater flexibility compared with the apical domain, as indicated by a higher average b-factor (Fig S4).

Table S2. X-ray data collection and refinement statistics.

Figure 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3. Crystal structure of the naïve EcHsp602 revealed a face-to-face symmetric conformation.

(A) Side view and top view of the overall structure of naïve EcHsp602. Chain A is colored by the domain and chain B is colored in silver. N- and C-termini of both subunits are labeled. (B) Superposition of the two protomers of the naïve EcHsp602. Coordinates are shown in cartoon and colored by RMSD values which are calculated by Pymol. (C) The interface residues between two protomers are shown in sphere and colored in deep orange and light blue for chains A and B, respectively. The atom types are colored in CPK to reveal the interacting forces. (D, E, F) The zoom-in views of the blue, green, and red boxes in (C), respectively. (D, E) focus on the interface between the α4 helices and their adjacent subunit, whereas (F) shows the top view of the interface at the apical domain. The residues involved in the interface are shown in sticks. The polar interactions are shown in yellow dash lines, and the residues involved in polar interaction are labeled. The chain ID of each residue and the α-helices are labeled in superscript.

Figure S3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S3. Sequence alignment of EcHsp60 with six chaperonin homologues.

The secondary structures of the naïve EcHsp607 structure are labeled above the sequences. MIS represents mitochondria importing signal. * indicates the residues involved in the formation of intermolecular polar contacts between EcHsp602 subunits. This figure is made by using the webserver ESPript.

Figure S4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S4. Thermal parameter (b-factor) distribution in the naïve EcHsp602 and EcHsp607 structures.

(A, B) The coordinates of (A) naïve EcHsp602 and (B) naïve EcHsp607 are colored by b-factor values and shown in putty implemented by Pymol. The red and thicker ribbon represents the residues with higher b-factor values, whereas the blue and thinner ribbon represents the opposite. The letters A, I, and E, indicate the apical, intermediate, and equatorial domains.

The analysis of the assembly interface revealed a favorable interaction between the two protomers of naïve EcHsp602, with a free energy of approximately −32.8 kcal/mol (Table S3). The interface between the two protomers, located at both the apical and equatorial domains, had a buried surface area of 3,943.8 Å2 (Fig 3C and Table S3). Although hydrophobic contacts were dominant at the interface, several polar interactions were also present (Tables S4 and S5). The α4 helix of each subunit protruded out from its equatorial domain to interact with the adjacent subunit, with the interface mainly composed of van der Waals forces and hydrophobic interactions (Fig 3D and E and Table S5). Two intermolecular polar contacts were identified on the α4 helix of each subunit, involving residues T89, E100, D103, R444, and D494 (Fig 3D and E and Table S4). These residues are conserved in various types of chaperonins, except for D103 (Fig S3). Interestingly, the inter-subunit interactions on the α4 helix differed between the two subunits, suggesting that α4 may have flexible contacts with the adjacent subunit in various binding modes. In contrast, the apical domain formed symmetrical contacts between the two protomers (Fig 3F and Table S4). The RLK motif (R264-K266) located at the α9 terminus formed four hydrogen bonds with residues from another protomer, including E212, R264, L265, and K266 (Fig 3F and Table S4). The RLK motif is conserved in animal mtHsp60, as revealed by sequence alignment analysis (Fig S3). These interacting residues contribute to the formation of the dimeric complex of EcHsp60.

Table S3. PISA analysis results of naïve EcHsp602 and EcHsp607.

Table S4. Intermolecular polar contacts between the subunits of naïve EcHsp60.

Table S5. Interacting residues between subunits in naïve EcHsp602.

Notably, a strong extra-density was identified near the dimer interface of naïve EcHsp602 in the Fo-Fc maps (higher than 10 σ) (Fig S5). This extra-density is surrounded by the side chains of two Y201 residues from both protomers and is near the RLK motif. Although the exact identity of the molecule(s) in this extra-density cannot be determined, it is suggested that they have a high occupancy and may play a role in the formation of dimeric EcHsp60. This crystal structure provides novel insight into the mechanism of dimerization of mtHsp60.

Figure S5.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S5. The extra density identified at the interface between the protomers in naïve EcHsp602.

(A, B) Side view (A) and top view (B) of the undefined extra-density in the middle of EcHsp602. The chains A and B of EcHsp602 were displayed in wheat and silver ribbons. Y201 and the residues involved in dimer association are shown in sticks and labeled. The chain ID of each residue is labeled in superscript. The Fo-Fc map at 5.0 σ near the Y201 is shown in green mesh.

Structural comparison between the dimeric and heptameric EcHsp60 reveals the impact of conformational changes on the ATP-binding pocket

To better understand the conformational changes between the dimeric and heptameric EcHsp60, we attempted to crystallize the naïve EcHsp607. Unfortunately, we obtained poorly diffracting crystals of naïve EcHsp607. As an alternative, we truncated the highly dynamic C-terminal GGM repeats of naïve EcHsp60 (Δ527–552), based on the crystallographic study of human mtHsp60 (Nisemblat et al, 2015). The resulting truncated protein, called naïve EcHsp60ΔC, was purified as a stable heptameric complex at both 4°C and RT (Fig S6A). Importantly, the heptameric naïve EcHsp60ΔC showed similar ATPase activity to the WT naïve EcHsp607 (Fig S6B), indicating that the C-terminal truncation did not significantly affect the protein structure. Therefore, we used these truncated naïve EcHsp60ΔC as the heptameric EcHsp607 for subsequent structural studies.

Figure S6.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S6. Naïve EcHsp60ΔC forms stable heptamers with ATPase activity.

(A) SEC analysis of the WT and C-terminal-truncated naïve EcHsp60s purified under room temperature (RT, red and brown) and 4°C (LT, blue and green), respectively. (B) The enzymatic kinetics of the WT naïve EcHsp607 (red) and the truncated naïve EcHsp60ΔC heptamers (green). Each point represents the mean of three repeated samples. Error bars indicate the standard deviations.

These truncated naïve EcHsp607 proteins were successfully crystallized, and the diffraction power of the crystals dramatically improved to a resolution of 3.5 Å (Table S2). The crystal structure of naïve EcHsp607 showed a typical heptameric, single-ring conformation in an asymmetric unit (Fig 4A). Unlike the partially disordered equatorial domain of naïve EcHsp602, most regions of naïve EcHsp607 could be observed in the electron density maps, except for the N-terminal MIS and two C-terminal residues (residues 525–526) (Fig 4B). The equatorial domain consists of 11 α-helices (α1-α5, α14-α19) and three sets of anti-parallel β-strands (β1/β16, β2/β3, β14/β15). The β1/β16 strands form a strong interaction with the β2/β3 strands of the neighboring protomer and stabilize the heptameric complex (Fig 4C). The thermal parameter distribution of the naïve EcHsp607 coordinate showed relatively low b-factor values in the equatorial domain compared with the apical and intermediate domains, suggesting that the equatorial domain is stabilized upon forming the heptameric conformation (Fig S4).

Figure 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 4. Crystal structure of the naïve EcHsp607 reveals a significant conformational difference at the equatorial domain compared with the naïve EcHsp602.

(A) The top and side views of naïve EcHsp607 structure. The coordinates are shown in cartoon and colored by chain. The molecular sizes are labeled. (B) The protomer is colored by the domain. N- and C-termini of both subunits are labeled. (C) EcHsp607 formed two anti-parallel β-strand pairs to connect the adjacent protomers. The residues involved in inter-molecular interaction are shown in sticks and labeled. (D) Superimpose of the protomers of naïve EcHsp602 (wheat) and naïve EcHsp607 (green). Significant conformational changes at the equatorial domain are zoomed in the right panel. The secondary structures were labeled to indicate the differences. (E) Structural comparison of the equatorial domains of the naïve EcHsp602 (wheat for chain A and silver for chain B) and naïve EcHsp607 (green). The residues involved in binding ATP are shown in sticks and colored in deep green (for naïve EcHsp607) and yellow (for naïve EcHsp602).

The structural comparison between the heptameric and dimeric EcHsp60 reveals differences in the equatorial domains, whereas the apical and intermediate domains showed similar conformations in both oligomeric states (Fig 4D). The α4 helix of naïve EcHsp607 was found to be folded into the core region of the equatorial domain with α1, α5, α16, and α20, whereas in naïve EcHsp602, the α4 helix extended out from the equatorial domain and interacts with the neighboring subunit (Fig 4D). In addition, the α1-3, α17-α19, and all β strands in the equatorial domain were only observed in naïve EcHsp607, suggesting that the equatorial domains may dynamically fold during the formation of the heptameric ring structure (Fig 4D).

To better understand the loss of ATPase activity in EcHsp602, the ATP binding sites were evaluated in the structures of naïve EcHsp602 and EcHsp607. In naïve EcHsp607, the ATP-binding motif, DGTTT, is located in the N-terminus of α4 helix and forms a pocket with G414 and D494 (Fig 4E). However, in EcHsp602, the α4 helix from the neighboring subunit is slightly twisted, resulting in the rearrangement of the ATP-binding residues. The T89 forms hydrogen bonds with D494, occupying the space of the ATP-binding pocket, and the D85, G86, and T87 are disordered, leading to the inability of EcHsp602 to hydrolyze ATP. These structural differences between naïve EcHsp602 and EcHsp607 provide an explanation for the inactivation mechanism of mtHsp60. The conformational dynamics of the equatorial domain may play a crucial role in the functional regulation of mtHsp60.

Moreover, we compared the structure of naïve EcHsp607 with well-studied chaperonins, including human mtHsp60 and E. coli GroEL (Fig S7). These proteins exhibit a similar ring-shaped arrangement of seven protomers (Fig S7A and D) and a comparable overall conformation, with slight variations in the apical domain (Fig S7B and E). The α4 helix is well folded in the equatorial domain of all heptameric chaperonins, and the β1/β16 and β2/β3 responsible for subunit interaction also have a similar conformation between these chaperonin heptamers (Fig S7C and F). These structural similarities support the idea that naïve EcHsp60, containing MIS, can still oligomerize into heptamers with a conserved protein conformation.

Figure S7.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S7. Structural comparisons of the naïve EcHsp607 and chaperonin homologues.

(A, B, C, D, E, F) The heptameric naïve EcHsp607 (green) was superimposed with the heptameric complexes of (A, B, C) human mtHsp60 (blue, PDB:7L7S) and (D, E, F) E. coli GroEL (yellow, PDB:1OEL). The top view of the heptamer (A, D); the side view of the protomer (B, E); and the bottom view of the protomer (C, F) shows the conserved protein conformations between naïve EcHsp607 and other type I chaperonins. The positions of α4 helix, β1/β16, and β2/β3 are labeled.

Decreased stability of dimeric interaction promotes the formation of heptamers in EcHsp60

The crystal structure of naïve EcHsp602 showed that both the α4 helix and the RLK motif contribute to forming inter-subunit interactions (Fig 3F). To further investigate the role of the RLK motif in the formation of dimeric EcHsp60, we generated point mutants (R264A and K266A) and a double mutant (R264AK266A) of the RLK motif. These mutants were expressed in E. coli and purified under both 4°C and RT conditions. However, the R264A mutant showed significant precipitation during purification, indicating that its solubility or stability may be affected. Therefore, only the K266A and R264AK266A mutants were further analyzed by SEC and compared with the WT naïve EcHsp60 (Fig 5A). The SEC results showed that the EcHsp607 peak was significantly increased in the K266A and R264AK266A mutants compared with the WT, whereas the peak of naïve EcHsp602 was dramatically decreased in the K266A and R264AK266A mutants. Furthermore, the retention volume of the dissociated EcHsp60 was slightly increased in the K266A and R264AK266A mutants, suggesting that these mutants may not form dimers but rather monomers (Fig 5A, blue triangle). The oligomeric conformation of the R264AK266A mutant was confirmed by negative-stain TEM, which showed that it formed a heptameric ring structure similar to the WT naïve EcHsp607 (Fig 5B and C). These results suggest that the RLK motif is crucial for stabilizing the dimeric form of EcHsp60.

Figure 5.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 5. The RLK motif is essential for maintaining the dimeric conformation of naïve EcHsp60.

(A) The WT and mutated naïve EcHsp60 were purified at 4°C for the SEC analysis. Black filled and open triangles represent the heptameric and the dimeric EcHsp60, respectively. The blue open triangle indicates the monomeric EcHsp60. (B) Negative-stain TEM micrograph of the R264AK266A heptamers in the SEC eluent. (C) Representative 2D classification results showed the single-ring heptameric conformation of R264AK266A. The class number of each particle is marked in the lower left corner. The classes #5 and #6 show the side view of the single-ringed heptamer. The position of the seven subunits in the class #1 particle is marked with yellow numbers.

Discussion

This study represents a significant advancement in our understanding of a novel conformation of chaperonin. The findings indicate that EcHsp60 can form a dimeric conformation in which the equatorial domain is partially reorganized, and the ATP binding pocket is disrupted, leading to an inactive state. The crystal structure showed that the RLK motif and the α4 helix play a crucial role in stabilizing the EcHsp60 dimer through intermolecular interactions. Upon incubation with ATP, the inactive dimer of EcHsp60 can be reconstituted into a heptameric ring complex, restoring its ATPase activity. This suggests that the dimeric complex is a temporary inactive state for EcHsp60.

Based on these findings, we have proposed a working model to elucidate the conformational transition during EcHsp60 oligomerization from dimer to heptamer (Fig 6). The α4 helix of each monomer is exchanged to link two protomers together in a face-to-face orientation to form a dimer (Fig 6A). The large conformational change at α4 helix disrupts the ATP-binding pocket and renders the dimeric EcHsp60 unable to bind ATP. The dimers might dynamically dissociate into individual monomers before forming the heptamers in a circular arrangement (Fig 6B). Upon ATP binding, the monomeric EcHsp60 forms a proper ATP-binding pocket with the refolded α4 helix in the equatorial domain, leading to the formation of the heptameric ring-shaped complex (Fig 6C). Our proposed model provides insight into the molecular mechanisms of the ATP-stimulated assembly and the complex instability of mtHsp60.

Figure 6.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 6. Proposed mechanism for the reassembly of EcHsp60 from an inactive dimer to active heptameric ring.

(A) EcHsp60 formed a homodimer through the interaction of RLK motifs and the protruded α4 helix. The EcHsp602 is sensitive to degradation because of the partial unfolded equatorial domain. (B) The dynamically dissociated EcHsp60 forms a well-folded equatorial domain with an ATP-binding pocket at the terminus of α4 helix. (C) The EcHsp60 monomers assemble into single-ring heptamers with the stable equatorial domain bound to ATP. The ATP binding (green circle) would stabilize the heptameric conformation and push the dynamical equilibrium to the right. A, I, and E represent the apical, intermediate, and equatorial domains, respectively. The gray and yellow colors indicate different protomers of EcHsp60.

In a previous study, it was shown that human naïve mtHsp60 could form stable heptamers in solution (Vilasi et al, 2014). Similarly, our study found that naïve EcHsp60 can form stable heptamers with comparable ATPase activity to mature EcHsp60. Structural analysis also revealed that the naïve EcHsp607 structure is similar to that of mature human mtHsp60. However, the SEC analysis showed that mature EcHsp60 forms a complex slightly larger than the heptameric complex but much smaller than 669 kD when purified at room temperature (Fig 1A). We proposed that this additional complex may be an octameric complex formed by a single-ring heptamer with a monomer in the central cavity (Klebl et al, 2021). The absence of octameric complexes in naïve EcHsp60 suggested that the presence of MIS may restrict substrate binding, despite not affecting the heptameric structure and ATPase activity. Although the MIS peptide was not observed in the naïve EcHsp607 because of its intrinsically disordered structure, it is expected to extend inside the heptameric ring and occupy the central cavity (Spinello et al, 2015). This cavity serves as a folding chamber for accommodating unfolded proteins during the folding process (Hartl, 1996). Therefore, the presence of MIS may limit the available space within the folding chamber of mtHsp60, potentially affecting its ability to bind to unfolded substrates. Further research is needed to examine the folding ability of naïve mtHsp60 to understand its role outside of the mitochondria.

Previous studies have reported the dissociation of both mitochondria and chloroplast Hsp60s into monomers at low protein concentrations, but there is limited structural information on monomeric chaperonins (Viitanen et al, 1998; Dickson et al, 2000; Parnas et al, 2009). In contrast, the prokaryotic chaperonin Mycobacterium tuberculosis Cpn60.2 (MtCpn60.2) has been observed to assemble into dimers in both solution and protein crystals, providing insights into the structure of dissociated chaperonins (Qamra & Mande, 2004; Shahar et al, 2011). Structural comparison between EcHsp60 and MtCpn60.2 revealed different arrangements of subunits in forming the dimeric complex (Fig S8). Specifically, the subunits in the naïve EcHsp602 dimer are symmetrically arranged, whereas the subunits of MtCpn60.2 interact through contacts between their apical and equatorial domains (Fig S8A–C). Compared with MtCpn60.2, naïve EcHsp602 forms a larger interface between subunits with more inter-subunit interactions, suggesting that EcHsp602 forms a more stable dimeric conformation. Nevertheless, in both EcHsp602 and MtCpn60.2, the N-terminal β1-3 and C-terminal β16 regions are not observed in the crystal structures (Fig S8D). These β-strands are responsible for connecting the subunits in the heptameric EcHsp607, and current structural evidence suggests that they may only form secondary structures upon oligomerization into the heptameric ring complex.

Figure S8.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure S8. The superposition of naïve EcHsp602 and the dimeric MtCpn60.2 show different subunit arrangements.

(A) Naïve EcHsp602 is colored by domain to show the two subunits interacting through the apical and equatorial domains. (B) The dimeric complex of MtCpn60.2 (PDB: 3RTK) is shown in cartoon with distinct colored domains. (C) The single subunit of the dimeric complex of MtCpn60.2 is compared with chain A of naïve EcHsp602 through superimposition. (D) Front and rear views of the superimposed naïve EcHsp602 and MtCpn60.2 monomers. (E) The interface between the subunits of MtCpn60.2. The residues forming inter-molecular hydrogen bonds of MtCpn60.2 are shown in sticks and labeled. The corresponding residues, K266 and Y201, in EcHsp60 are also shown in stick for comparison.

Furthermore, this study highlights a novel function of the apical domain in the chaperonin structure. In E. coli GroEL, the α8 and α9 helices are responsible for substrate recognition and binding by interacting with hydrophobic patches of the substrate peptides (Buckle et al, 1997; Chen & Sigler, 1999). In contrast, in dimeric EcHsp60, these helices contribute to inter-subunit interactions on the apical domain (Fig 3). In addition, the α9 helix also participates in the dimer formation of MtCpn60.2 (Fig S8E). Residue R266 in MtCpn60.2, corresponding to K266 in EcHsp60, forms hydrogen bonds with another subunit in the dimeric complex. These findings suggest that the apical domain could interact with various peptides to perform diverse functions, emphasizing the significance of the apical domain in chaperonin functions.

Overall, this study provides useful insights into the structural and functional aspects of mtHsp60. The identification of a novel dimeric conformation improves our knowledge on the molecular mechanisms of mtHsp60 assembly and reactivation. These findings contribute to our understanding of the chaperonin-mediated protein folding regulation. Further research is needed to fully elucidate the physiological functions of mtHsp60 in cells and to explore its potential as a therapeutic target.

Materials and Methods

Plasmids

The cDNA library of grouper fishes was prepared according to previous studies (Hsu et al, 2013). The -cDNA encoding EcHSP60 was amplified and cloned into the pET24a (Invitrogen) vector between the restriction enzyme cutting sites, NdeI and XhoI. The constructed plasmid was named pET-EcHSP60 and was used to express the recombinant EcHSP60 fused with a C-terminal six-His tag. To express the C-terminal truncated EcHSP60 removing the flexible GGM tail, the DNA region encoding E527 to G552 was removed from pET-EcHSP60 to form pET-EcHsp60ΔC using In-Fusion seamless cloning kit (Takara Bio). In addition, the plasmids used to express the R264A, K266A, and R264AK266A mutants were also constructed by using the In-Fusion seamless cloning kit based on pET-EcHSP60 as the template.

Protein expression and purification

The constructed plasmids, pET-EcHsp60 and pET-EcHsp60ΔC, were respectively transformed into E. coli Rosetta (DE3) strain for large-scale protein production. The protein expression was induced by adding 0.5 mM IPTG into the E. coli culture during the mid-log phase at 30°C for 6 h. After EcHsp60 expression, the E. coli culture was harvested and resuspended in the lysis buffer (25 mM Tris–HCl, pH 7.6, 300 mM NaCl, 0.1 mM PMSF). Ultrasonication was employed for cell homogenization with a power intensity of 960 J/ml and a duty cycle of 1 s (UP200S; Hielscher). The EcHSP602 was homogenized and purified at 4°C, whereas EcHSP607 was carried out at room temperature. The C-terminal His-tagged EcHsp60 was purified using Ni-NTA columns on an FPLC system (ÄKTA pure; Cytiva). The purified proteins were further concentrated for SEC purification using the SEC buffer (25 mM Tris–HCl, pH 7.6 and 150 mM NaCl) at a flow rate of 1 ml/min. The size-exclusion column (Superdex S200pg, 16/600; Cytiva) was used for the large-scale preparation of heptameric and dimeric EcHSP60. For analytical preparation of mutant EcHsp60s, the high-resolution SEC column (Superdex 200 Increase 10/300 GL; Cytiva) was used instead. Purified EcHSP60 was concentrated to a final concentration of 10 mg/ml using a centrifuge concentrator (Amicon Ultra-15, 30 kD MWCO; Millipore). The concentrated EcHSP602 and EcHSP607 were stored at 4°C and room temperature, respectively, for following studies.

Cross-linking assay

The cross-linking assay was performed with slight modifications to previously published protocols (Parnas et al, 2009; Shi et al, 2017). The dimeric EcHsp60 was dialyzed into the assay medium (4.56 mM NaH2PO4, 20.44 mM Na2HPO4, and 300 mM NaCl, pH 7.6) and concentrated to 0.6 mg/ml for addition of the bis(sulfosuccinimidyl)suberate (BS3; Thermo Fisher Scientific). For each reaction, 30 μl of protein and 10 μl of BS3 were mixed to give a final concentration of BS3 from 0.15 to 0.6 mM and incubated at 37°C for 30 min. After incubation, the cross-link reaction was quenched by adding 10 μl stop solution (1 M Tris–HCl, pH 8.0), and the samples were analyzed by 10% SDS–PAGE.

ATP hydrolysis assay

The ATP hydrolysis assay is according to previous studies with minor modifications (Lin et al, 2012). Twenty micrograms of EcHSP60 were mixed with an assay medium (50 mM Tris–HCl, pH 7.5, 20 mM KCl, and 1 mM MgCl2) containing various concentrations of ATP in a final volume of 90 μl and incubated at 37°C for 30 min. The reactions were stopped by adding 180 μl stop solution (0.7% [wt/vol] ammonium molybdate, 0.02% [wt/vol] 1-amino-2-naphthol4-sulfonic acid, 2.0% [wt/vol] sodium dodecyl sulfate, and 1.16 N HCl). The amount of Pi released in each sample was quantified by measuring the absorbance at 700 nm using a microplate reader (Multiskan SkyHigh; Thermo Fisher Scientific). Twofold serial dilutions of Pi standards were used to generate the calibration curve in the concentration range of 4–128 μM. Each sample was repeated three times to calculate the mean and SD. The kinetic curves were obtained by fitting the experimental data with a nonlinear regression equation of the substrate inhibition function using the software, GraphPad Prism 8.0. The Khalf and Vmax values with standard error were calculated from the fitting equations.

Limited trypsin digestion

Aliquots of EcHSP602 and EcHSP607 were diluted in the protein buffer (25 mM Tris, 150 mM NaCl, pH 7.6) containing 50 units of trypsin to a final protein concentration of 1 mg/ml and incubated at 25°C for various time courses. The reactions were stopped by mixing with SDS–PAGE loading buffer to denature the trypsin and EcHsp60. All samples were analyzed by 10% SDS–PAGE and Western blot to detect the residual amount of EcHsp60. The primary and secondary antibodies are anti-His mouse antibody and anti-mouse IgG goat antibody, respectively.

EcHSP60 assembly assay

The heptamer assembly method used for EcHsp60 is based on the reconstitution assay of oligomeric human mtHsp60 with some minor modifications (Viitanen et al, 1998). The purified EcHsp602 was diluted to 1.2 mg/ml in the assay medium (50 mM Tris, NaCl, pH 7.5, 300 mM NaCl, 20 mM Mg(CH3COO)2, and 20 mM KCl) containing various concentrations of ATP to trigger the assembly process. The samples were incubated at 30°C for 2 h, and then each sample was injected with 500 μl for SEC analysis using an FPLC system (ÄKTA pure; Cytiva) with the SEC column (Superdex 200pg 10/600; Cytiva). The SEC buffer (25 mM Tris–HCl, pH 7.6, and 150 mM NaCl) was used at a flow rate of 0.5 ml/min. UV absorbance was monitored at 280 nm. The eluents containing EcHSP60 were fraction-collected for further measurements of the ATPase activity.

Protein crystallization

To crystallize EcHsp60, the EcHsp602 and EcHsp607 proteins were purified and concentrated to 50 mg/ml and 40 mg/ml in the SEC buffer (25 mM Tris–HCl, pH 7.6, and 150 mM NaCl), respectively. Initial crystallization conditions were identified from robotic screening using the hanging-drop vapor diffusion method at 20°C. Protein crystals were obtained from Wizard classic 2 NO. 48 (Rigaku) for EcHsp602 and from MemGold2 G2 (Molecular dimension) for EcHsp607. The optimized crystal condition for EcHSP602 is 400 mM potassium sodium tartrate and 21% (wt/vol) PEG 3350, whereas for heptamer, it is 50 mM Tris–HCl, 500 mM MgCl2, 10.5% (wt/vol) PEG1000. The crystals of both proteins were grown in 7 d at 20°C.

X-ray diffraction and data refinement

Diffraction data of the EcHsp602 and EcHsp607 crystals were collected at beamline BL13B1 of NSRRC. Each diffraction image was exposed for 20 s under a low-temperature nitrogen stream with a 1° oscillation for a total of 180 images. The diffraction data were indexed, integrated, and scaled using HKL2000 (Otwinowski & Minor, 1997). Molecular Replacement (MR) was conducted by using Phaser implemented in the Phenix to obtain the correct phase (Liebschner et al, 2019). The search model was the predicted model generated by SWISS-MODEL using human mtHsp60 (PDB:6MRD) as a template (Gomez-Llorente et al, 2020). The models obtained from phasing solutions were further refined and validated using Phenix and Coot. Ridge body refinement was first introduced to refine the initial models derived from MR. The “secondary structure restrains” and “Ramachandra restrains” are applied to maintain the geometry for heptameric EcHsp60. TLS restrain refinement was then applied to reduce the Rfree for both sets of diffraction data. The models after refinements are visualized using Pymol and Chimera (Pettersen et al, 2004). The inter-molecular polar interactions of the refined models were identified by PDBePISA analysis (Krissinel & Henrick, 2007).

Negative-stain TEM and single particle analysis

The purified oligomeric EcHSP60 was diluted to 1 mg/ml, and 4 μl samples were placed on the glow discharged grids (Formvar/carbon-coated Cu grids, 400 mesh) for 60 s and then removed by filter paper blotting. The grids were washed by 8 μl ddH2O drops for two times, and 2% (wt/vol) uranyl acetate was added to stain the grids for 60 s. After removal of the staining solution, the grid was air-dried completely for TEM analysis. The micrographs were acquired using a TEM (JEOL JEM-1400) at an electron voltage of 100 kV. Micrographs processed with CTF Estimation were used for automatic particle picking using cisTEM (Grant et al, 2018). The picked particles were then used for 2D classification and to build the initial map using cryoSPARC (Punjani et al, 2017). The initial map was further refined with C7 symmetry using homogeneous refinement. The density map fitted with mtHSP60 coordinates was displayed by using Chimera (Pettersen et al, 2004). For WT EcHsp60 heptamers, 24,177 particles were picked from 61 micrographs, and 15,685 particles selected after two runs of 2D classification were used to build the initial map. For R264AK266A heptamers, 18,626 particles were picked from 40 micrographs, and 12,051 particles remained after the 2D classifications.

Data Availability

The structural coordinates and structure factors of the dimeric and heptameric EcHsp60 have been deposited in the PDB (http://www.rcsb.org/) with the following accession numbers: 7V9R—heptameric naïve EcHsp60; 7V98—dimeric naïve EcHsp60.

Acknowledgements

We appreciate the experimental facility and the technical services provided by the Synchrotron Radiation Protein Crystallography Core Facility (SPXF) of the National Core Facility for Biopharmaceuticals (NCFB), Ministry of Science and Technology (MOST), and the National Synchrotron Radiation Research Center (NSRRC), a national user facility funded by the Ministry of Science and Technology (MOST), Taiwan (R.O.C.). Initial crystallization screening was carried out at the NSRRC-NCKU Protein Crystallography Laboratory at University Center for Bioscience and Biotechnology of National Cheng Kung University (funding by NSRRC 11123LAB02). We gratefully acknowledge the use of TEM instruments (EM000900 of MOST 110-2731-M-006-001) belonging to the Core Facility Center of National Cheng Kung University. We would like to acknowledge funding from the Ministry of Science and Technology (MOST) of Taiwan (MOST 109-2636-B-006 -012; MOST 110-2636-B-006 -012; MOST 111-2636-B-006 -012) to S-M Lin.

Author Contributions

  • M-C Lai: data curation, formal analysis, and writing—original draft.

  • H-Y Cheng: data curation and formal analysis.

  • S-H Lew: data curation and formal analysis.

  • Y-A Chen: data curation, software, and formal analysis.

  • C-H Yu: conceptualization, resources, and writing—review and editing.

  • H-Y Lin: conceptualization, methodology, and writing—review and editing.

  • S-M Lin: conceptualization, supervision, and writing—original draft, review, and editing.

Conflict of Interest Statement

The authors declare that they have no conflict of interest that could have influenced the results or interpretation of the findings presented in this study. All authors have participated in the design, execution, and analysis of the research and have approved the final manuscript for submission. No external funding or support was received from organizations that could have influenced the outcome of the study in any way.

  • Received October 5, 2022.
  • Revision received March 16, 2023.
  • Accepted March 16, 2023.
  • © 2023 Lai et al.
Creative Commons logoCreative Commons logohttps://creativecommons.org/licenses/by/4.0/

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

References

  1. ↵
    1. Boisvert DC,
    2. Wang J,
    3. Otwinowski Z,
    4. Norwich AL,
    5. Sigler PB
    (1996) The 2.4 Å crystal structure of the bacterial chaperonin GroEL complexed with ATP gamma S. Nat Struct Biol 3: 170–177. doi:10.1038/nsb0296-170
    OpenUrlCrossRefPubMed
  2. ↵
    1. Buckle AM,
    2. Zahn R,
    3. Fersht AR
    (1997) A structural model for GroEL–polypeptide recognition. Proc Natl Acad Sci U S A 94: 3571–3575. doi:10.1073/pnas.94.8.3571
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Bukau B,
    2. Horwich AL
    (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92: 351–366. doi:10.1016/s0092-8674(00)80928-9
    OpenUrlCrossRefPubMed
  4. ↵
    1. Chen L,
    2. Sigler PB
    (1999) The crystal structure of a GroEL/peptide complex. Cell 99: 757–768. doi:10.1016/s0092-8674(00)81673-6
    OpenUrlCrossRefPubMed
  5. ↵
    1. Cheng MY,
    2. Hartl FU,
    3. Martin J,
    4. Pollock RA,
    5. Kalousek F,
    6. Neuper W,
    7. Hallberg EM,
    8. Hallberg RL,
    9. Horwich AL
    (1989) Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620–625. doi:10.1038/337620a0
    OpenUrlCrossRefPubMed
  6. ↵
    1. Dickson R,
    2. Weiss C,
    3. Howard RJ,
    4. Alldrick SP,
    5. Ellis RJ,
    6. Lorimer G,
    7. Azem A,
    8. Viitanen PV
    (2000) Reconstitution of higher plant chloroplast chaperonin 60 tetradecamers active in protein folding. J Biol Chem 275: 11829–11835. doi:10.1074/jbc.275.16.11829
    OpenUrlAbstract/FREE Full Text
  7. ↵
    1. Frydman J
    (2001) Folding of newly translated proteins in vivo: The role of molecular chaperones. Annu Rev Biochem 70: 603–647. doi:10.1146/annurev.biochem.70.1.603
    OpenUrlCrossRefPubMed
  8. ↵
    1. Gomez-Llorente Y,
    2. Jebara F,
    3. Patra M,
    4. Malik R,
    5. Nisemblat S,
    6. Chomsky-Hecht O,
    7. Parnas A,
    8. Azem A,
    9. Hirsch JA,
    10. Ubarretxena-Belandia I
    (2020) Structural basis for active single and double ring complexes in human mitochondrial Hsp60-Hsp10 chaperonin. Nat Commun 11: 1916. doi:10.1038/s41467-020-15698-8
    OpenUrlCrossRef
  9. ↵
    1. Grant T,
    2. Rohou A,
    3. Grigorieff N
    (2018) cisTEM, user-friendly software for single-particle image processing. Elife 7: e35383. doi:10.7554/eLife.35383
    OpenUrlCrossRefPubMed
  10. ↵
    1. Gupta RS
    (1995) Evolution of the chaperonin families (HSP60, HSP 10 and TCP-1) of proteins and the origin of eukaryotic cells. Mol Microbiol 15: 1–11. doi:10.1111/j.1365-2958.1995.tb02216.x
    OpenUrlCrossRefPubMed
  11. ↵
    1. Hartl FU
    (1996) Molecular chaperones in cellular protein folding. Nature 381: 571–580. doi:10.1038/381571a0
    OpenUrlCrossRefPubMed
  12. ↵
    1. Horwich AL,
    2. Weber-Ban EU,
    3. Finley D
    (1999) Chaperone rings in protein folding and degradation. Proc Natl Acad Sci U S A 96: 11033–11040. doi:10.1073/pnas.96.20.11033
    OpenUrlAbstract/FREE Full Text
  13. ↵
    1. Horwich AL,
    2. Fenton WA,
    3. Chapman E,
    4. Farr GW
    (2007) Two families of chaperonin: Physiology and mechanism. Annu Rev Cell Dev Biol 23: 115–145. doi:10.1146/annurev.cellbio.23.090506.123555
    OpenUrlCrossRefPubMed
  14. ↵
    1. Hsu Y-J,
    2. Hou C-Y,
    3. Lin S-J,
    4. Kuo W-C,
    5. Lin H-T,
    6. Lin JH-Y
    (2013) The biofunction of orange-spotted grouper (Epinephelus coioides) CC chemokine ligand 4 (CCL4) in innate and adaptive immunity. Fish Shellfish Immunol 35: 1891–1898. doi:10.1016/j.fsi.2013.09.020
    OpenUrlCrossRef
  15. ↵
    1. Klebl DP,
    2. Feasey MC,
    3. Hesketh EL,
    4. Ranson NA,
    5. Wurdak H,
    6. Sobott F,
    7. Bon RS,
    8. Muench SP
    (2021) Cryo-EM structure of human mitochondrial HSPD1. iScience 24: 102022. doi:10.1016/j.isci.2020.102022
    OpenUrlCrossRef
  16. ↵
    1. Krissinel E,
    2. Henrick K
    (2007) Inference of macromolecular assemblies from crystalline state. J Mol Biol 372: 774–797. doi:10.1016/j.jmb.2007.05.022
    OpenUrlCrossRefPubMed
  17. ↵
    1. Levy-Rimler G,
    2. Viitanen P,
    3. Weiss C,
    4. Sharkia R,
    5. Greenberg A,
    6. Niv A,
    7. Lustig A,
    8. Delarea Y,
    9. Azem A
    (2001) The effect of nucleotides and mitochondrial chaperonin 10 on the structure and chaperone activity of mitochondrial chaperonin 60. Eur J Biochem 268: 3465–3472. doi:10.1046/j.1432-1327.2001.02243.x
    OpenUrlCrossRefPubMed
  18. ↵
    1. Liebschner D,
    2. Afonine PV,
    3. Baker ML,
    4. Bunkoczi G,
    5. Chen VB,
    6. Croll TI,
    7. Hintze B,
    8. Hung L-W,
    9. Jain S,
    10. McCoy AJ, et al.
    (2019) Macromolecular structure determination using X-rays, neutrons and electrons: Recent developments in Phenix. Acta Crystallogr D Struct Biol 75: 861–877. doi:10.1107/S2059798319011471
    OpenUrlCrossRefPubMed
  19. ↵
    1. Lin S-M,
    2. Tsai J-Y,
    3. Hsiao C-D,
    4. Huang Y-T,
    5. Chiu C-L,
    6. Liu M-H,
    7. Tung J-Y,
    8. Liu T-H,
    9. Pan R-L,
    10. Sun Y-J
    (2012) Crystal structure of a membrane-embedded H+-translocating pyrophosphatase. Nature 484: 399–403. doi:10.1038/nature10963
    OpenUrlCrossRefPubMed
  20. ↵
    1. Llorca O,
    2. McCormack EA,
    3. Hynes G,
    4. Grantham J,
    5. Cordell J,
    6. Carrascosa JL,
    7. Willison KR,
    8. Fernandez JJ,
    9. Valpuesta JM
    (1999) Eukaryotic type II chaperonin CCT interacts with actin through specific subunits. Nature 402: 693–696. doi:10.1038/45294
    OpenUrlCrossRefPubMed
  21. ↵
    1. Nielsen KL,
    2. Cowan NJ
    (1998) A single ring is sufficient for productive chaperonin-mediated folding in vivo. Mol Cell 2: 93–99. doi:10.1016/s1097-2765(00)80117-3
    OpenUrlCrossRefPubMed
  22. ↵
    1. Nisemblat S,
    2. Yaniv O,
    3. Parnas A,
    4. Frolow F,
    5. Azem A
    (2015) Crystal structure of the human mitochondrial chaperonin symmetrical football complex. Proc Natl Acad Sci U S A 112: 6044–6049. doi:10.1073/pnas.1411718112
    OpenUrlAbstract/FREE Full Text
  23. ↵
    1. Ostermann J,
    2. Horwich AL,
    3. Neupert W,
    4. Hartl FU
    (1989) Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis. Nature 341: 125–130. doi:10.1038/341125a0
    OpenUrlCrossRefPubMed
  24. ↵
    1. Otwinowski Z,
    2. Minor W
    (1997) Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol 276: 307–326. doi:10.1016/S0076-6879(97)76066-X
    OpenUrlCrossRefPubMed
  25. ↵
    1. Parnas A,
    2. Nadler M,
    3. Nisemblat S,
    4. Horovitz A,
    5. Mandel H,
    6. Azem A
    (2009) The MitCHAP-60 disease is due to entropic destabilization of the human mitochondrial Hsp60 oligomer. J Biol Chem 284: 28198–28203. doi:10.1074/jbc.M109.031997
    OpenUrlAbstract/FREE Full Text
  26. ↵
    1. Pettersen EF,
    2. Goddard TD,
    3. Huang CC,
    4. Couch GS,
    5. Greenblatt DM,
    6. Meng EC,
    7. Ferrin TE
    (2004) UCSF Chimera--a visualization system for exploratory research and analysis. J Comput Chem 25: 1605–1612. doi:10.1002/jcc.20084
    OpenUrlCrossRefPubMed
  27. ↵
    1. Prasad TK,
    2. Hack E,
    3. Hallberg RL
    (1990) Function of the maize mitochondrial chaperonin hsp60: Specific association between hsp60 and newly synthesized F1-ATPase alpha subunits. Mol Cell Biol 10: 3979–3986. doi:10.1128/mcb.10.8.3979-3986.1990
    OpenUrlAbstract/FREE Full Text
  28. ↵
    1. Punjani A,
    2. Rubinstein JL,
    3. Fleet DJ,
    4. Brubaker MA
    (2017) cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat Methods 14: 290–296. doi:10.1038/nmeth.4169
    OpenUrlCrossRefPubMed
  29. ↵
    1. Qamra R,
    2. Mande SC
    (2004) Crystal structure of the 65-kilodalton heat shock protein, chaperonin 60.2, of Mycobacterium tuberculosis. J Bacteriol 186: 8105–8113. doi:10.1128/JB.186.23.8105-8113.2004
    OpenUrlAbstract/FREE Full Text
  30. ↵
    1. Ricci C,
    2. Ortore MG,
    3. Vilasi S,
    4. Carrotta R,
    5. Mangione MR,
    6. Bulone D,
    7. Librizzi F,
    8. Spinozzi F,
    9. Burgio G,
    10. Amenitsch H, et al.
    (2016) Stability and disassembly properties of human naïve Hsp60 and bacterial GroEL chaperonins. Biophysical Chem 208: 68–75. doi:10.1016/j.bpc.2015.07.006
    OpenUrlCrossRef
  31. ↵
    1. Shahar A,
    2. Melamed-Frank M,
    3. Kashi Y,
    4. Shimon L,
    5. Adir N
    (2011) The dimeric structure of the Cpn60.2 chaperonin of Mycobacterium tuberculosis at 2.8 Å reveals possible modes of function. J Mol Biol 412: 192–203. doi:10.1016/j.jmb.2011.07.026
    OpenUrlCrossRefPubMed
  32. ↵
    1. Shi J-M,
    2. Pei J,
    3. Liu E-Q,
    4. Zhang L
    (2017) Bis(sulfosuccinimidyl) suberate (BS3) crosslinking analysis of the behavior of amyloid-β peptide in solution and in phospholipid membranes. PLoS One 12: e0173871. doi:10.1371/journal.pone.0173871
    OpenUrlCrossRef
  33. ↵
    1. Singh B,
    2. Patel HV,
    3. Ridley RG,
    4. Freeman KB,
    5. Gupta RS
    (1990) Mitochondrial import of the human chaperonin (HSP60) protein. Biochem Biophys Res Commun 169: 391–396. doi:10.1016/0006-291x(90)90344-m
    OpenUrlCrossRefPubMed
  34. ↵
    1. Spinello A,
    2. Ortore MG,
    3. Spinozzi F,
    4. Ricci C,
    5. Barone G,
    6. Gammazza AM,
    7. Piccionello AP
    (2015) Quaternary structures of GroEL and naïve-hsp60 chaperonins in solution: A combined SAXS-MD study. RSC Adv 5: 49871–49879. doi:10.1039/c5ra05144d
    OpenUrlCrossRef
  35. ↵
    1. Viitanen PV,
    2. Lorimer GH,
    3. Seetharam R,
    4. Gupta RS,
    5. Oppenheim J,
    6. Thomas JO,
    7. Cowan NJ
    (1992) Mammalian mitochondrial chaperonin 60 functions as a single toroidal ring. J Biol Chem 267: 695–698. doi:10.1016/S0021-9258(18)48338-X
    OpenUrlAbstract/FREE Full Text
  36. ↵
    1. Viitanen PV,
    2. Lorimer G,
    3. Bergmeier W,
    4. Weiss C,
    5. Kessel M,
    6. Goloubinoff P
    (1998) Purification of mammalian mitochondrial chaperonin 60 through in vitro reconstitution of active oligomers. Methods Enzymol 290: 203–217. doi:10.1016/s0076-6879(98)90020-9
    OpenUrlCrossRefPubMed
  37. ↵
    1. Vilasi S,
    2. Carrotta R,
    3. Mangione MR,
    4. Campanella C,
    5. Librizzi F,
    6. Randazzo L,
    7. Martorana V,
    8. Marino Gammazza A,
    9. Ortore MG,
    10. Vilasi A, et al.
    (2014) Human Hsp60 with its mitochondrial import signal occurs in solution as heptamers and tetradecamers remarkably stable over a wide range of concentrations. PLoS One 9: e97657. doi:10.1371/journal.pone.0097657
    OpenUrlCrossRef
  38. ↵
    1. Vilasi S,
    2. Bulone D,
    3. Caruso Bavisotto C,
    4. Campanella C,
    5. Marino Gammazza A,
    6. San Biagio PL,
    7. Cappello F,
    8. Conway de Macario E,
    9. Macario AJL
    (2017) Chaperonin of group I: Oligomeric spectrum and biochemical and biological implications. Front Mol Biosci 4: 99. doi:10.3389/fmolb.2017.00099
    OpenUrlCrossRef
  39. ↵
    1. Voth W,
    2. Jakob U
    (2017) Stress-activated chaperones: A first line of defense. Trends Biochem Sci 42: 899–913. doi:10.1016/j.tibs.2017.08.006
    OpenUrlCrossRef
  40. ↵
    1. Wang JC-Y,
    2. Chen L
    (2021) Structural basis for the structural dynamics of human mitochondrial chaperonin mHsp60. Sci Rep 11: 14809. doi:10.1038/s41598-021-94236-y
    OpenUrlCrossRef
  41. ↵
    1. Zeilstra-Ryalls J,
    2. Fayet O,
    3. Georgopoulos C
    (1991) The universally conserved GroE (Hsp60) chaperonins. Annu Rev Microbiol 45: 301–325. doi:10.1146/annurev.mi.45.100191.001505
    OpenUrlCrossRefPubMed
  42. ↵
    1. Zhao Q,
    2. Liu C
    (2017) Chloroplast chaperonin: An intricate protein folding machine for photosynthesis. Front Mol Biosci 4: 98. doi:10.3389/fmolb.2017.00098
    OpenUrlCrossRef
PreviousNext
Back to top
Download PDF
Email Article

Thank you for your interest in spreading the word on Life Science Alliance.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Crystal structures of dimeric and heptameric mtHsp60 reveal the mechanism of chaperonin inactivation
(Your Name) has sent you a message from Life Science Alliance
(Your Name) thought you would like to see the Life Science Alliance web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Dimeric mtHsp60 with Inactive ATPase Activity
Meng-Cheng Lai, Hao-Yu Cheng, Sin-Hong Lew, Yu-An Chen, Chien-Hung Yu, Han-You Lin, Shih-Ming Lin
Life Science Alliance Mar 2023, 6 (6) e202201753; DOI: 10.26508/lsa.202201753

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Dimeric mtHsp60 with Inactive ATPase Activity
Meng-Cheng Lai, Hao-Yu Cheng, Sin-Hong Lew, Yu-An Chen, Chien-Hung Yu, Han-You Lin, Shih-Ming Lin
Life Science Alliance Mar 2023, 6 (6) e202201753; DOI: 10.26508/lsa.202201753
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
Issue Cover

In this Issue

Volume 6, No. 6
June 2023
  • Table of Contents
  • Cover (PDF)
  • About the Cover
  • Masthead (PDF)
Advertisement

Jump to section

  • Article
    • Abstract
    • Introduction
    • Results
    • Discussion
    • Materials and Methods
    • Data Availability
    • Acknowledgements
    • References
  • Figures & Data
  • Info
  • Metrics
  • Reviewer Comments
  • PDF

Subjects

  • Structural Biology

Related Articles

  • No related articles found.

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • Genome-wide modeling of fly metabolism
  • Modeling IPF in iPSC-derived alveolar organoids
  • H3K4me3 and multigenerational neuronal homeostasis
Show more Research Article

Similar Articles

EMBO Press LogoRockefeller University Press LogoCold Spring Harbor Logo

Content

  • Home
  • Newest Articles
  • Current Issue
  • Archive
  • Subject Collections

For Authors

  • Submit a Manuscript
  • Author Guidelines
  • License, copyright, Fee

Other Services

  • Alerts
  • Twitter
  • RSS Feeds

More Information

  • Editors & Staff
  • Reviewer Guidelines
  • Feedback
  • Licensing and Reuse
  • Privacy Policy

ISSN: 2575-1077
© 2023 Life Science Alliance LLC

Life Science Alliance is registered as a trademark in the U.S. Patent and Trade Mark Office and in the European Union Intellectual Property Office.